Results 171 to 180 of about 4,180 (199)

Mechanism of Transcriptional Activation by NtrC

2014
In the case of nitrogen regulatory protein C (NtrC), both nucleotide hydrolysis and transcriptional activation depend on phosphorylation of an aspartate residue in the N-terminal receiver domain of the protein (also called its regulatory domain). In this chapter, the authors review the evidence that the NtrC protein from enteric bacteria, which is a ...
Susan C. Porter, Anne K. North, Sydney Kustu   +2 more
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Allostery and Folding of the N-terminal Receiver Domain of Protein NtrC

The Journal of Physical Chemistry B, 2013
The N-terminal receiver domain of protein NtrC (NtrC(r)) exhibits allosteric transitions between the inactive (unphosphorylated) and active (phosphorylated) state on the microsecond time scale. Using a coarse-grained variational model with coupled energy basins, we illustrate that significant loss of conformational flexibility is the key determinant of
Swarnendu, Tripathi, John J, Portman
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Molecular Dynamic Simulations of the N-Terminal Receiver Domain of NtrC Reveal Intrinsic Conformational Flexibility in the Inactive State

Journal of Biomolecular Structure and Dynamics, 2006
The N-terminal receiver domain of NtrC is the molecular switch in the two-component signal transduction. It is the first protein where structures of both the active (phosphyroylated) and inactive (unphosphyroylated) states are determined experimentally ...
Yongmei Wang
exaly   +2 more sources

Urea Utilization in Rhodobacter capsulatus is Regulated by NtrC

2005
The phototrophic non-sulphur purple bacterium R. capsulatus can use urea as sole source of nitrogen. Five mutants (Xan-9, Xan-10, Xan-19, Xan-20, and Xan-22) unable to grow with urea as the only N source were isolated by random transposon Tn5 mutagenesis (Leimkuhler et al., 1998).
B. Masepohl, B. Kaiser, N. Isakovic, W. Klipp   +3 more
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Unusual Oligomerization Required for Activity of NtrC, a Bacterial Enhancer-Binding Protein

Science, 1997
Nitrogen regulatory protein C (NtrC) contacts a bacterial RNA polymerase from distant enhancers by means of DNA loops and activates transcription by allowing polymerase to gain access to the template DNA strand. It was shown that NtrC from Salmonella typhimurium must build large oligomers to activate transcription ...
C, Wyman, I, Rombel, A K, North, C, Bustamante, S, Kustu   +4 more
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Glutamine auxotrophs with mutations in a nitrogen regulatory gene, ntrC, that is near glnA

Molecular and General Genetics MGG, 1981
Some mutations to glutamine auxotrophy in the 86 unit region of the Salmonella chromosome lie within the nitrogen regulatory gene, ntrC, rather than the structural gene encoding glutamine synthetase, glnA, Assignment of mutations to ntrC is based on fine structure mapping by P22-mediated transduction and on complementation analysis.
G R, Wei, S, Kustu
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Three-dimensional solution structure of the N-terminal receiver domain of NTRC

Biochemistry, 1995
NTRC is a transcriptional enhancer binding protein whose N-terminal domain is a member of the family of receiver domains of two-component regulatory systems. Using 3D and 4D NMR spectroscopy, we have completed the 1H, 15N, and 13C assignments and determined the solution structure of the N-terminal receiver domain of the NTRC protein.
B F, Volkman, M J, Nohaile, N K, Amy, S, Kustu, D E, Wemmer   +4 more
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Interaction of purified NtrC protein with nitrogen regulated promoters from Klebsiella pneumoniae

Molecular and General Genetics MGG, 1985
The product of the Klebsiella pneumoniae nitrogen regulatory gene ntrC has been purified and shown to be a dimeric protein of subunit molecular weight 54Kd, designated NtrC. In an in vitro coupled transcription-translation system NtrC inhibited expression from both the ntrBC and glnA promoters. NtrC bound to both of these ntr repressible promoters with
T, Hawkes, M, Merrick, R, Dixon
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The role of NtrC in the adaptation of Herbaspirillum seropedicae SmR1 to nitrogen limitation and to nitrate

Archives of Microbiology
The RNA-Seq profiling of Herbaspirillum seropedicae SmR1 wild-type and ntrC mutant was performed under aerobic and three nitrogen conditions (ammonium limitation, ammonium shock, and nitrate shock) to identify the major metabolic pathways modulated by these nitrogen sources and those dependent on NtrC. Under ammonium limitation, H.
Paloma, Bonato, Doumit, Camilios-Neto, Michelle Zibetti, Tadra-Sfeir, Francisco J Teles, Mota, Marcelo, Muller-Santos, Roseli, Wassem, Emanuel Maltempi, de Souza, Fábio, de Oliveira Pedrosa, Leda Satie, Chubatsu   +8 more
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Activation of the Rhizobium leguminosarum glnII gene by NtrC is dependent on upstream DNA sequences

Molecular and General Genetics MGG, 1992
The cloning and sequence determination is reported of the DNA region of Rhizobium leguminosarum coding for glutamine synthetase II (GSII). An open reading frame (ORF) encoding 326 amino acids was defined as the glnII gene on the basis of its similarity to other glnII genes and the ability of a DNA fragment carrying this ORF to complement the glutamine ...
E J, Patriarca, M, Chiurazzi, G, Manco, A, Riccio, A, Lamberti, A, De Paolis, M, Rossi, R, Defez, M, Iaccarino   +8 more
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