Results 121 to 130 of about 1,179,647 (210)
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Comparison of gene transcription in cloned bovine embryos produced by different nuclear transfer techniques

Molecular Reproduction and Development, 2001
AbstractThe efficiency of animal production using cloning technology is still relatively low and research to determine a more efficient nuclear transfer procedure is ongoing. One approach which may be informative in assessing the viability of nuclear transfer embryos is the analysis of embryonic gene expression.
R, Daniels   +4 more
semanticscholar   +5 more sources

Simplification of Bovine Somatic Cell Nuclear Transfer by Application of a Zona-Free Manipulation Technique

open access: closedCloning and Stem Cells, 2001
Contemporary nuclear transfer techniques often require the involvement of skilled personnel and extended periods of micromanipulation. Here, we present details of the development of a nuclear transfer technique for somatic cells that is both simpler and faster than traditional methods.
P.J. Booth   +4 more
semanticscholar   +6 more sources

Comparison of intra- and interspecies nuclear transfer techniques in the production of cloned caprine embryos

open access: closedSmall Ruminant Research, 2011
Abstract Among the ARTs that are applied in goat farming industry, reproductive cloning technology in production of cloned goat embryos are foreseen to facilitate the effort of mass goat production in just a short time frame. There are two possible approaches that can be applied to produce cloned embryos, namely intraspecies SCNT (intraSCNT) and ...
Rina Abdullah   +2 more
semanticscholar   +4 more sources

Development of the techniques for nuclear transfer in pigs

Theriogenology, 1999
Nuclear transfer in pigs was developed in the late 1980's. The techniques were based on previous studies in frogs, mice and cattle. Within stage nuclear transfer, pronuclear exchange, was followed by the transfer of nuclei from cleavage stage embryos. While these have resulted in term development, many problems remain.
R S, Prather, T, Tao, Z, Macháty
openaire   +3 more sources

Modifications of chemically induced-enucleated nuclear transfer technique by reverse-order nuclear transfer in mouse

open access: closedZygote, 2009
SummaryTo improve the developmental potential of somatic cell cloned embryos derived from demecolcine (DC) induced-enucleated nuclear transfer (INT), we modified the INT procedures by transferring donor nuclei into recipient cytoplasts prior to the induced enucleation of the recipient cytoplasts, and we called this modified INT technique as reverse ...
Yongsheng Wang   +9 more
openalex   +3 more sources

Evaluation of Lumped Parameter Heat Transfer Techniques for Nuclear Reactor Applications

open access: closedNuclear Science and Engineering, 1973
An analytical study was performed to investigate the utility and accuracy of the lumped parameter approach to heat transfer analysis in a nuclear reactor subassembly.
David M. France, T. Ginsberg
openalex   +2 more sources

Techniques for Nuclear Transfer to Mouse Embryonic Stem Cells

open access: closed, 2006
In this chapter, methods are described that permit the enucleation of mouse embryonic stem (ES) cells and the transfer of donor nuclei to these cells before or after enucleation has taken place. The small size and high nucleus-to-cytoplasm volume ratio of ES cells poses a challenge to their enucleation.
Danièle Pralong, Paul J. Verma
openalex   +3 more sources

Development of somatic cell nuclear transfer technique for biomedical and agricultural research (year II) : report

open access: closed, 2005
There are two objectives of the somatic nuclear transfer studies. The first objective was to evaluate the possibility to produce the inter-species cloned embryos from donor cells of different animals transfer to rabbit, pig or swamp buffalo oocytes. The second objective was to study the donor cell type can significantly affect the in vitro development ...
Mongkol Techakumphu   +5 more
openalex   +2 more sources

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