Results 121 to 130 of about 6,135,044 (385)

An efficient strategy for producing RNA‐free Nucleocapsid protein of SARS‐CoV‐2 for biochemical and structural investigations

FEBS Open Bio, EarlyView.
Cleavable N‐terminal Thioredoxin fusion enabled soluble expression and purification of otherwise insoluble SARS‐CoV‐2 Nucleocapsid (N) protein. A four‐step purification strategy yielded highly homogeneous, RNA‐free N protein. Binding assays showed high RNA affinity (Kd ~ 28 nm). The study will facilitate high‐resolution structural studies of N protein,
Shweta Singh, Gagan D. Gupta
wiley   +1 more source

Plasma microRNA Signature as Predictive Marker of Clinical Response to Therapy During Multiple Sclerosis

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Despite the availability of effective therapies for Multiple Sclerosis (MS), the unpredictable nature of disease progression and the variability in individual treatment outcomes call for reliable biomarkers. This pilot study aims to investigate the potential of plasma circulating microRNAs (miRNAs) as predictive biomarkers for ...
Fortunata Carbone, Alessandra Colamatteo, Teresa Micillo, Gianmarco Abbadessa, Silvia Garavelli, Clorinda Fusco, Claudia Russo, Francesco Perna, Federica Garziano, Claudia La Rocca, Maria Mottola, Giorgia Teresa Maniscalco, Simona Bonavita, Antonio Luca Spiezia, Alessia Castiello, Roberta Lanzillo, Vincenzo Brescia Morra, Claudio Procaccini, Paola de Candia, Giuseppe Matarese   +19 more
wiley   +1 more source

Peptide–Oligonucleotide Conjugation: Chemistry and Therapeutic Applications

Current Issues in Molecular Biology
Oligonucleotides have been identified as powerful therapeutics for treating genetic disorders and diseases related to epigenetic factors such as metabolic and immunological dysfunctions.
Anna L. Malinowska, Harley L. Huynh, Sritama Bose   +2 more
doaj   +1 more source

Selective Targeting to Glioma with Nucleic Acid Aptamers [PDF]

, 2015
Malignant glioma is characterised by a rapid growth rate and high capacity for invasive infiltration to surrounding brain tissue; hence, diagnosis and treatment is difficult and patient survival is poor.
Alder, Jane Elizabeth, Aptekar, Shraddha Ashok, Arora, Mphit, Ashton, K, Dawson, Tim, Lawrence, Clare Louise, Lea, Robert William, Shaw, Lisa   +7 more
core   +3 more sources

Targeting RAGE with Nanobodies for Molecular Imaging of Cancers and Alzheimer's Disease

Advanced Biology, EarlyView.
RAGE‐specific nanobodies were isolated via phage display and characterized by ELISA, cell ELISA, and SPR. In vivo imaging in renal carcinoma and Alzheimer's disease mouse models demonstrated that NbF8, the highest‐affinity clone, selectively targeted RAGE‐overexpressing tumors and brain tissues, highlighting its potential as a molecular imaging agent ...
Guangfeng Liang, Fujing Wang, Wei Xiong, Guangwei Shi, Junling Yuan, Yang Li, Hongyan Zhang, Yanmei Xing, Shan Jin, Kongjun Yang, Zhongliang Dai, Jichao Sun, Zhijie Li, Jianhong Wang   +13 more
wiley   +1 more source

Novel Signal-Enhancing Approaches for Optical Detection of Nucleic Acids—Going beyond Target Amplification

Chemosensors, 2015
Detection of low-abundance nucleic acids is a challenging task, which over the last two decades has been solved using enzymatic target amplification.
Laura Miotke, Maria Carla Barducci, Kira Astakhova   +2 more
doaj   +1 more source

DNA-coated microcrystals [PDF]

, 2005
Coprecipitation leads to self-assembly of bioactive DNA on the surface of salt, sugar or amino-acid crystals and provides a rapid inexpensive immobilization method suitable for preparing dry-powder formulations of nucleic acids, useful for storage ...
Barry D. Moore, Franks, Förster, Geeta Fuglevand, Kreiner, Li, Mann, Marie-Claire Parker, Michaela Kreiner, Murugesan, Nassiopoulou, Niemeyer, Shalaev, Tang, Wang   +14 more
core   +1 more source

Self-Awareness: Nucleic Acid-Driven Inflammation and the Type I Interferonopathies.

Annual Review of Immunology, 2019
Recognition of foreign nucleic acids is the primary mechanism by which a type I interferon-mediated antiviral response is triggered. Given that human cells are replete with DNA and RNA, this evolutionary strategy poses an inherent biological challenge, i.
C. Uggenti, A. Lepelley, Y. Crow
semanticscholar   +1 more source

The Potential for Extracellular Vesicles in Nanomedicine: A Review of Recent Advancements and Challenges Ahead

Advanced Biology, EarlyView.
Extracellular vesicles (EVs) play a dual role in diagnostics and therapeutics, offering innovative solutions for treating cancer, cardiovascular, neurodegenerative, and orthopedic diseases. This review highlights EVs’ potential to revolutionize personalized medicine through specific applications in disease detection and treatment.
Farbod Ebrahimi, Anjali Kumari, Samaneh Ghadami, Saqer Al Abdullah, Kristen Dellinger   +4 more
wiley   +1 more source

DNA Tweezers with Replaceable Clamps for the Targeted Degradation of Cell Membrane Proteins

Pharmaceutics
Background: Cell membrane proteins play crucial roles in signal transduction and nutrient transport. Many membrane proteins are reportedly overexpressed in cancer cells, which is closely related to cancer progression.
Yang Sun, Yichen Huang, Daiquan Chen, Shangjiu Hu, Tao Pan, Yuanding Liu, Ruowen Wang, Weihong Tan   +7 more
doaj   +1 more source