Results 181 to 190 of about 78,833 (285)

Rapid Microbiological Diagnostics for Sepsis: Narrative Review of Current and Prospective Approaches. [PDF]

Crit Care Explor
Harrison LB, Sohani ZN, Lasry D, Cheng MP, Lee TC, Babiker A, Kadri SS, Lawandi A.   +7 more
europepmc   +1 more source

Nonviral Gold Nanoparticle‐Mediated Delivery of CRISPR‐Cas9 Ribonucleoprotein and Long DNA Transgenes Into Primary Blood Cells

Advanced NanoBiomed Research, EarlyView.
CRISPR/Cas9 has revolutionized the field of gene therapy, but delivery remains an outstanding issue. We propose a nonviral gold‐nanoparticle platform for co‐delivery of CRISPR/Cas9 ribonucleoprotein and long 2.1 kilobase dsDNA transgene constructs. This CRISPR‐AuNP is inexpensive to produce and mediate gene editing and DNA delivery in T cells and CD34+
Rachel A. Cunningham, Karthikeya S. V. Gottimukkala, Daniel D. Lane, Katrina Poljakov, Patricia Lipson, Mark R. Enstrom, Alessandro Rizzi, Aude G. Chapuis, Jennifer E. Adair   +8 more
wiley   +1 more source

Garland Rolling Circle Amplification Mediated Self-Priming Extension Strategy for Sensitive and Label-Free <i>Pseudomonas aeruginosa</i> Analysis in Perioperative Period. [PDF]

J Microbiol Biotechnol
Xu Y, Wei L.
europepmc   +1 more source

Plant Virus Immunotherapy for HPV‐Associated Oropharyngeal Squamous Cell Carcinoma

Advanced NanoBiomed Research, EarlyView.
Cowpea mosaic virus (CPMV) nanoparticles are safe and potent cancer immunotherapy candidates with strong immunomodulatory activity. CPMV activates innate immunity, which in turn drives adaptive immune responses and promotes durable, systemic antitumor effects.
Jessica Fernanda Affonso de Oliveira, Patrick Opdensteinen, Andrea Simms, Seongwon Jung, Giuliana P. Mognol, Hui Chen, Judith A. Varner, Nicole F. Steinmetz   +7 more
wiley   +1 more source

Trends of nucleic acid - based point-of-care diagnostics for infectious diseases. [PDF]

J Biol Eng
Hatem H, Mysara M, Ramadan R.
europepmc   +1 more source

Ultrasensitive non-enzymatic protein detection using proximity immunoassay with photonic resonator absorption microscopy. [PDF]

NPJ Biosens
Shepherd S, Bhaskar S, Xu H, Zhang H, Liu W, Cunningham BT.   +5 more
europepmc   +1 more source

To culture or not to culture: correlating <i>Neisseria gonorrhoeae</i> culture positivity with nucleic acid amplification test cycle threshold values to promote cost-effective gonococcal resistance surveillance. [PDF]

Sex Transm Infect
Osbak KK, Twisk DE, van Westreenen M, Klaassen C, Götz HM.   +4 more
europepmc   +1 more source

Establishment of a rapid and highly sensitive direct-RAA-RDB detection platform: application in non-deletion α-thalassemia. [PDF]

Front Mol Biosci
Wei Y, Zhao Y, Ye C, Xie M, Zhou X, Ma J, Liu X, Qing J, Chen Z.   +8 more
europepmc   +1 more source

Establishment and application of a quadruple target real-time PCR assay for detecting Yersinia pestis. [PDF]

PLoS One
Wang W, Zhang X, Yu H, Wang Z.
europepmc   +1 more source

Dual‐Channel Interdigitated Aptamer‐Based Sensors for Rapid Small‐Molecule Detection in Biofluids

Angewandte Chemie International Edition, EarlyView.
An electrochemical aptamer sensing chip employs dual interdigitated electrodes and selective self‐assembled monolayer removal to convert small‐molecule recognition into robust “signal‐off/on” responses. This dual‐channel signal conversion minimizes background noise and accelerates probe diffusion, enabling rapid (as fast as 5–30 min), low‐volume ...
Senyao Wang, Ali Elmorsy, Defne Tüzün, Lilly Schmidt, Sebastian Freko, Lukas Hiendlmeier, Chen Wang, Alonso Ingar Romero, George Al Boustani, Hu Peng, Berna Özkale, Nako Nakatsuka, Bernhard Wolfrum   +12 more
wiley   +1 more source