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Validation criteria for nucleic acid amplification techniques for bacterial infections
Clinical Chemistry and Laboratory Medicine, 2008Nucleic acid techniques (NATs), such as species-specific and universal polymerase chain reactions (PCRs), are finding ever wider use in the diagnosis of bacterial infection. However, although universal PCR assays, in particular, approach a type of modern Petri dish, they have a number of limitations which restrict their applicability.
Gerd, Döring +2 more
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Hairpin DNA-Mediated isothermal amplification (HDMIA) techniques for nucleic acid testing
Talanta, 2021Nucleic acid detection is of great importance in a variety of areas, from life science and clinical diagnosis to environmental monitoring and food safety. Unfortunately, nucleic acid targets are always found in trace amounts and their response signals are difficult to be detected. Amplification mechanisms are then practically needed to either duplicate
Idorenyin A. Iwe +3 more
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Chemiluminescent sensors based on nucleic acid amplification techniques and functional nucleic acids
Journal of LuminescenceXu Teng, Rongxian Huang, Yaru Liang
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An Introduction to In Vitro Nucleic Acid Amplification Techniques
2012A molecular microbiology detection procedure consists of three basic components: (1) nucleic acid extraction, (2) amplification, and (3) detection/identification. Over the past two decades, the development and optimization of a series of in vitro nucleic acid amplification (NAA) technologies has opened new avenues in diagnostic microbiology for the ...
Haijing Li, Yi-Wei Tang
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New nucleic acid amplification techniques in diagnostic pathology
Journal of the European Academy of Dermatology and Venereology, 1994AbstractIn 1985, Mullis discovered an exponentially increasing amplification system for nucleic acids, which became known as the polymerase chain reaction (PCR). This method permits the selective in vitro amplification of a particular DNA region by mimicking phenomena of in vivo DNA replication.
J.J. O'Leary +6 more
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Mycoses, 2020
AbstractBackgroundFungal infections have increased during the last years due to the AIDS epidemic and immunosuppressive therapies. The available diagnostic methods, such as culture, histopathology and serology, have several drawbacks regarding sensitivity, specificity and time‐consuming, while molecular methods are still expensive and dependent on many
Matheus Silva Zatti +2 more
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AbstractBackgroundFungal infections have increased during the last years due to the AIDS epidemic and immunosuppressive therapies. The available diagnostic methods, such as culture, histopathology and serology, have several drawbacks regarding sensitivity, specificity and time‐consuming, while molecular methods are still expensive and dependent on many
Matheus Silva Zatti +2 more
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International Journal of STD and AIDS, 2009
The objective of the study was to evaluate the concordance between nucleic acid amplification technique (NAAT) and culture for the diagnosis of Neisseria gonorrhoeae among attendees at a genitourinary medicine clinic in East London. All patients testing positive for N.
S, Creighton, B, Revell, A, Barrow
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The objective of the study was to evaluate the concordance between nucleic acid amplification technique (NAAT) and culture for the diagnosis of Neisseria gonorrhoeae among attendees at a genitourinary medicine clinic in East London. All patients testing positive for N.
S, Creighton, B, Revell, A, Barrow
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Isothermal Amplification Techniques for the Detection of Nucleic Acids and Proteins
2020The early detection of disease is beneficial for improved patient prognoses. One major challenge in early disease detection is the small, undetectable amounts of biomarkers. Detection strategies that confer amplification of the biomarker itself or of the detection signal are therefore desirable. Furthermore, emerging strategies conferring amplification
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Amplification-Based Nucleic Acid Scanning Techniques to Assess Genetic Polymorphism inCandida
Critical Reviews in Microbiology, 2003Opportunistic pathogen Candida causes common fungal infections that manifest both superficially and systemically, especially in compromised patients. Although C. albicans is by far the main etiological agent of candidosis, the frequency of isolation of other non-albicans species such as C. glabrata and C.
Dassanayake, RS, Samaranayake, LP
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Analytical Biochemistry, 1998
Recent advances in nucleic acid amplification techniques have allowed for quantitation of viral nucleic acid levels in clinical specimens. The most prevalent testing is carried out for HIV viral load. Strand displacement amplification (SDA) is an isothermal DNA amplification system utilizing a restriction enzyme and a DNA polymerase with strand ...
C M, Nycz +4 more
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Recent advances in nucleic acid amplification techniques have allowed for quantitation of viral nucleic acid levels in clinical specimens. The most prevalent testing is carried out for HIV viral load. Strand displacement amplification (SDA) is an isothermal DNA amplification system utilizing a restriction enzyme and a DNA polymerase with strand ...
C M, Nycz +4 more
openaire +2 more sources