Results 91 to 100 of about 67,468 (297)

Antimicrobial Efficacy of Honey’s Flavonoids and Phenolic Acids: Implications for Wound Healing and Pharmaceutical Development

Wah Academia Journal of Health and Nutrition
The antimicrobial nature of honey has been appreciated for thousands of years; however, the molecular mechanism through which its function is only now being unraveled.
Faiqa Shakeel, Ihsan Ali Rind, Zara Ijaz, Mahnoor Dua Kotwal, Sanam Nawaz, Maria, Hiba Noor, Syeda Rubab Toqeer   +7 more
doaj   +1 more source

Comparison of different protocols for the bovine viral diarrhea virus detection by RT-PCR in pools of whole blood and blood serum artificially contaminated/
Comparação de diferentes protocolos para a detecção do vírus da diarréia viral bovina por RT-PCR em grupos de sangue total e de soro sangüíneo, artificialmente contaminados

Semina: Ciências Agrárias, 2005
The RT-PCR technique was optimized and evaluated to detect the 5’ untranslated region of bovine viral diarrhea virus (BVDV) from clinical samples that consisted of blood serum and whole blood artificially contaminated with the NADL strain of BVDV.
Amauri A. Alfieri, Alice F. Alfieri, Daniela Pilz   +2 more
doaj   +1 more source

TET1 Inhibition Promotes Therapeutic Sensitivity in TP53‐Mutant GBM by Influencing Genome Fragility and Altering TAMs Biology

Advanced Science, EarlyView.
In TP53mut GBM cells, reduced P53 function is associated with increased TET1 expression. Genetic or pharmacological inhibition of TET1 correlates with genome fragility, including DNA damage, cellular senescence, telomere shortening, and reactive oxygen species accumulation, which may contribute to increased efficacy of antitumor therapy.
Zhuonan Pu, Jinqiu Liu, Yuxuan Deng, Shuyu Hao, Xiaoli Zhang, Mingxu Yang, Chao Guo, Chao Du, Yingdan Chen, Tai Sun, Nan Ji, Zhengping Zhuang, Jie Feng   +12 more
wiley   +1 more source

Systematic Position Mapping of Split CRISPR‐Cas12a Activators Enables Highly Sensitive miRNA Detection and Cancer Cell Stratification

Advanced Science, EarlyView.
ABSTRACT Amplification‐free Cas12a diagnostics with split crRNA enable rapid and programmable target recognition, yet insufficient understanding of DNA activator architecture prevents predictable control over trans‐cleavage activity and sensitivity. Here we systematically map over 200 split DNA activator configurations by introducing nicks at every ...
Xiaoyan Tang, Zhe Li, Yuning Lu, Miao Ma, Zijian Mo, Jiajun Ke, Xinyu Luan, Tiangang Luan, Junqiu Zhai   +8 more
wiley   +1 more source

Synthesis and Biophysical Properties of C5-Functionalized LNA (Locked Nucleic Acid) [PDF]

, 2015
Oligonucleotides modified with conformationally restricted nucleotides such as locked nucleic acid (LNA) monomers are used extensively in molecular biology and medicinal chemistry to modulate gene expression at the RNA level.
Bharat Baral (27019), Michael E. Østergaard (628019), Dale C. Guenther (1321689), Pawan K. Sharma (805741), Brooke A. Anderson (1321683), Patrick J. Hrdlicka (1321695), Mamta Kaura (1321686), Pawan Kumar (162916), Daniel J. Raible (1321692)   +8 more
core   +1 more source

Probiotic‐Derived Vesicles Rectally Delivered via Thermo‐Gelling Copolymer Promote Mucosal Healing and Reduce Inflammation in Ulcerative Colitis

Advanced Science, EarlyView.
A thermo‐responsive rectal delivery platform integrating probiotic‐derived extracellular vesicles within a hyaluronic acid‐based copolymer enables localized and sustained therapy for ulcerative colitis. The formulation effectively suppresses inflammation, promotes macrophage polarization toward a regenerative phenotype, restores epithelial barrier ...
Ayushi Mairal, Ubaid Tariq, Shreya Mehrotra, Saravanan Matheshwaran, Jan Marsal, Ashok Kumar   +5 more
wiley   +1 more source

Synthesis and Properties of Nucleic Acid Analogues Consisting of a Benzene−Phosphate Backbone [PDF]

, 2016
The synthesis and properties of a nucleic acid analogue consisting of a benzene−phosphate backbone are described. The building blocks of the nucleic acid analogue are composed of bis(hydroxymethyl)benzene residues connected to nucleobases via the biaryl ...
Aya Shibata (1907263), Takumi Kato (2669029), Takumi Inoue (2669032), Yukio Kitade (283049), Yoshihito Ueno (74202), Yasutomo Ito (1537672), Mahito Yoshida (2669026), Masahiro Ebihara (1575001), Kumiko Sato (2015764)   +8 more
core   +1 more source

Site‐Specific Disulfide‐Mediated Crosslinking of DNA Nanocubes for Enhanced Biological Applications

Small Science
The development of DNA nanotechnology has enabled the creation of diverse nanomaterials with significant potential in biological applications, such as sensing or drug delivery.
Sinan Faiad, Quentin Laurent, Jathavan Asohan, Tyler Brown, Alexander Prinzen, Hanadi Farouk Sleiman   +5 more
doaj   +1 more source

Computation and Structure‐Guided Arginine Scanning Engineers a Hyperactive AP Endonuclease for Multiplex Viral RNA Sensing

Advanced Science, EarlyView.
Computational and structure‐guided arginine scanning rewires the DNA‐binding interface of APE1 to create APE1‐Evo, a hyperactive yet specific AP endonuclease. Integrated into the NAPTUNE‐V2.0 cascade, APE1‐Evo enables amplification‐free, multiplex viral RNA sensing for dengue virus and influenza A/B, highlighting a general strategy for engineering ...
Junlan Wang, Ting Wu, Feizuo Wang, Boyang Wang, Xin Yu Wong, Senfeng Zhang, Shengsheng Ma, Yao Liu, Fangyao Yao, Zhifeng Zeng, Siyuan Yang, Kunming Liu, Qi Wang, Yilong Wang, Yunxiang Zang, Xiaolong Wang, Xingxu Huang, Shao Li, Quanquan Ji, Tao Hu, Chunyi Hu   +20 more
wiley   +1 more source

Enhanced-ice-COLD-PCR for the Sensitive Detection of Rare DNA Methylation Patterns in Liquid Biopsies

Bio-Protocol, 2019
In the context of precision medicine, the identification of novel biomarkers for the diagnosis of disease, prognosis, predicting treatment outcome and monitoring of treatment success is of great importance.
Florence Mauger, Jörg Tost
doaj   +1 more source