Results 111 to 120 of about 67,468 (297)

A Novel Continuous-Flow PCR Microdevice Operated by a Single Heat Source

Micromachines
This paper presents a constant-temperature, single-heat-source continuous-flow PCR (CF-PCR) microdevice that achieves stable thermal control for denaturation, annealing, and extension on a single platform. Key innovations include: (1) a metal-powder/PDMS
Weining Song, Di Wu, Yutong Xing, Wenming Wu   +3 more
doaj   +1 more source

Implantable electrochemical sensors for continuous blood monitoring

FlexMat, EarlyView.
Implantable electrochemical sensors enable direct, in situ, and continuous blood monitoring, shifting diagnosis from population‐based averages to dynamic, individualized analysis. This review systematically examines the influence of the unique blood properties on sensor design.
Kuangyi Zou, Yuanzhen Wang, Haidong Wang, Ye Zhang   +3 more
wiley   +1 more source

Nucleic acid-functionalized iron oxide nanoparticles for controlled drug release using magnetic hyperthermia [PDF]

, 2019
Resumen del trabajo presentado a la 12th International Conference on the Scientific and Clinical Applications of Magnetic Carriers, celebrada en Copenhague (Dinamarca) del 22 al 26 de mayo de 2018.The use of nanoparticles to deliver drugs to tumours ...
Fratila, Raluca M., Herrero, Vanessa, Fuente, Jesús M. de la, Idiago López, Francisco Javier   +3 more
core  

Multilabeled Pyrene-Functionalized 2‘-Amino-LNA Probes for Nucleic Acid Detection in Homogeneous Fluorescence Assays [PDF]

, 2016
Homogeneous fluorescence assays for detection of nucleic acids are widely used in biological sciences. Typically, probes such as molecular beacons that rely on distance-dependent fluorescence quenching are used for such assays.
Jesper Wengel (20049), Mads D. Sørensen (27188), Patrick J. Hrdlicka (1321695), Niels Harrit (2164564), B. Ravindra Babu (27189)   +4 more
core   +1 more source

Ordered assembly of Cas12a for better performance of CRISPR/Cas system with application to the diagnosis of dental caries

Interdisciplinary Medicine, EarlyView.
Here, we present a tetrahedral DNA framework (TDF)‐mediated Cas12a ordered assembly strategy. TDFs arrange CRISPR/Cas12a into ordered structures controllably, enhancing catalytic efficiency through localized Cas12a hotspots while maintaining spatial intervals to facilitate substrate diffusion.
Yining Zhao, Panpan Qiao, Yujing Zeng, Murtaza Sayed, Qin Mi, Juan Zhang, Genxi Li   +6 more
wiley   +1 more source

5-(Pyren-1-yl)uracil as a Base-Discriminating Fluorescent Nucleobase in Pyrrolidinyl Peptide Nucleic Acids [PDF]

, 2011
A pyrene-labeled uridine (UPy) monomer for a pyrrolidinyl peptide nucleic acid with an alternating proline/2-aminocyclopentanecarboxylic acid backbone (acpcPNA) was synthesized and incorporated into the PNA.
Chotima Vilaivan, Wagenknecht, Hans‐Achim, Tirayut Vilaivan, Boonlua, Chalothorn, Vilaivan, Chotima, Vilaivan, Tirayut, Hans-Achim Wagenknecht, Chalothorn Boonlua   +7 more
core   +1 more source

Controllable DNA Condensation by Mixed Cationic Gemini/Nonionic Surfactant Systems: Structure, Thermodynamics, and Cytotoxicity

Journal of Surfactants and Detergents, EarlyView.
ABSTRACT Controllable DNA condensation is important for nucleic‐acid packaging and non‐viral gene‐delivery systems. This study aimed to determine how the composition of mixed nonionic C12E10 and cationic gemini C12C6C12Br2 surfactants regulates DNA condensation, structure, thermodynamics, and cytotoxicity.
Bo Wang, Linyi Zhu, Yaxun Fan, Yilin Wang   +3 more
wiley   +1 more source

Salt‐induced nutritional and metabolic shifts in halophytes: implications for food security

Journal of the Science of Food and Agriculture, EarlyView.
Abstract Plant species vary in their response to salinity: some crops show a degree of salt tolerance, while halophytes – whether wild or cultivated – are characterized by a high capacity to thrive under saline conditions. Halophytes are considered a source of valuable secondary metabolites with potential economic value, yet they might also produce ...
Giulia Atzori, Pedro Garcia Caparros, Antonella Castagna, Luisa Custódio, Valentina Lazazzara, Claus Krogh Madsen, Felicia Menicucci, Maria João Rodrigues, Katalin Solymosi, Jose Ramon Acosta Motos   +9 more
wiley   +1 more source

Eco-synthesized silver nanoparticles from Curcuma longa leaves: Phytochemical and biomedical applications

Next Nanotechnology
Nanotechnology has revolutionized biomedical sciences, with silver nanoparticles (AgNPs) gaining attention for their antimicrobial, antioxidant, and anti-inflammatory properties. However, conventional synthesis methods often involve toxic chemicals.
Sapam Riches Singh, S.J. Akshatha, Megha B. Abbigeri, Akshata Choudhari Padti, Santosh Mallikarjun Bhavi, Sudheendra Rao Kulkarni, Bothe Thokchom, Asmatanzeem Bepari, Ramesh Babu Yarajarla, B.S. Srinath   +9 more
doaj   +1 more source

Deubiquitinase USP38 Stabilizes PLK1 Expression to Boost DNA Damage Repair in Ovarian Cancer

The Kaohsiung Journal of Medical Sciences, EarlyView.
ABSTRACT Ovarian cancer (OC) is one of the most prevalent and severe gynecological malignant tumors. DNA damage repair (DDR) is essential in maintaining genome stability. This study aims to investigate the effects and mechanisms of USP38 and PLK1 on DNA damage repair and malignant behavior in OC cells.
Yuan Ma, Ying Li, Kai‐Li Li
wiley   +1 more source