Results 121 to 130 of about 67,468 (297)

LPCAT3 as a Potential Drug Target for Ultraviolet Radiation–Induced Cataract: Insights From Multiomics Analysis

The Kaohsiung Journal of Medical Sciences, EarlyView.
ABSTRACT Ultraviolet B (UVB) radiation is a major risk factor for cataract development, but the molecular mechanisms underlying this process, particularly the involvement of regulated cell death pathways such as ferroptosis, remain unclear. Transcriptomic, proteomic, and metabolomic analyses were performed on lens tissues from UVB‐induced cataract rat ...
Fei Xu, Xiao‐Bo Wan, Yong‐Shun Liang, Tian Lan, Hao Liang   +4 more
wiley   +1 more source

From the sea to DNA barcode: Optimized preservation method for gelatinous zooplankton integrative taxonomy

Limnology and Oceanography: Methods, EarlyView.
Abstract Assigning taxonomic identity and thus assessing taxonomic diversity in gelatinous zooplankton is challenging due to their fragility and difficult preservation. Formalin fixation compromises DNA integrity, while flash‐freezing, ethanol, and acetone preservation affect morphological features. In this study, we developed a methodological protocol
Claudia Traboni, Rade Garić, Rita Marino, Paolo Sordino, Priscilla Licandro   +4 more
wiley   +1 more source

Exploring eco‐evolutionary and temporal patterns of arbuscular mycorrhizal fungal communities colonizing Sorghum bicolor across sites of contrasting land use history and climate

PLANTS, PEOPLE, PLANET, EarlyView.
Sorghum bicolor is a globally important cereal crop with annual yields exceeding 50 million tons across more than 100 countries and can be grown on marginal lands where conventional agriculture is limited. We examined how eight genetically diverse sorghum genotypes shaped arbuscular mycorrhizal fungal (AMF) community assembly across two contrasting ...
Philip Brailey‐Crane, Sedona M. Spann, Thomas H. Pendergast IV, Ben J. Long, Ashton K. Brinkley, Sarah R. Mondibrown, Nancy C. Johnson, Katrien M. Devos, Jeffrey L. Bennetzen   +8 more
wiley   +1 more source

Structural and Stability Analysis of de Novo Designed Proteins Incorporating a Plant Self‐Incompatibility Motif

Proteins: Structure, Function, and Bioinformatics, EarlyView.
ABSTRACT Artificial protein design enables the creation of stable scaffolds beyond those evolved in nature. Incorporation of native functional motifs into de novo scaffolds provides a promising strategy to mimic natural interactions while altering structural frameworks.
Hakuto Miyoshi, Koya Sakuma, Yoshitaka Moriwaki, Naoki Aoyama, Toma Kashima, Tohru Terada, Akimasa Miyanaga, Shinya Fushinobu   +7 more
wiley   +1 more source

Single and dual RPA‐CRISPR/Cas assays for point‐of‐need detection of Stewart's wilt pathogen (Pantoea stewartii subsp. stewartii) of corn and Maize dwarf mosaic virus

Pest Management Science, Volume 81, Issue 4, Page 1988-1999, April 2025.
Schematic diagram of the single and dual RPA‐CRISPR/Cas12a/13a diagnostic assays for the detection of Pantoea stewartii subsp. stewartii and Maize dwarf mosaic virus. The validated assays provide a useful and sensitive molecular tool for detecting two quarantine pathogens of maize within a minimal resource framework suitable for fast‐tracking the ...
Qian Tian, Hua Zhou, Zhenxing Zhao, Yongjiang Zhang, Wenjun Zhao, Lulu Cai, Tao Guo   +6 more
wiley   +1 more source

Defining Composition–Cytokine Relationships Enables the Design of Lipid Nanoparticles with Programmed Immunogenicity

Small, EarlyView.
Lipid nanoparticle composition governs distinct cytokine programs through the interplay of ionizable and polyethylene glycol‐conjugated (PEGylated) lipids. In vitro profiling, design of experiments (DoE) modeling, and in vivo validation reveal how formulation can tune reactogenicity‐associated pathways, while transgene expression remains the strongest ...
Adam Alexander Walters, Belal I. Hanafy, Chuan‐En Lu, Sara Marelli, Alaa Zam, Kai Liu, Rachel E. Foreman, Ramesh Soundararajan, Philippe Collin, Lennart Lindfors, Alan Sabirsh, Mariarosa Mazza, Khuloud T. Al‐Jamal, Stephanie M. Bates, George Thom, Jan Rehwinkel, Alexander N Kapustin   +16 more
wiley   +1 more source

Synthesis of aromatic peptide nucleic acids [PDF]

, 1999
The synthesis of two analogues of Aromatic Peptide Nucleic Acids (APNAs) monomers was successfully achieved. These Peptide Nucleic Acid analogues could be used as building blocks for the synthesis of oligomers with the DNA and RNA recognition properties ...
Boyd, Michael J
core  

Biomaterial‐Integrated Electroporation for Therapeutic Delivery: From Gene Editing to Tumor Ablation and Immune Modulation

Small, EarlyView.
Biomaterial‐integrated electroporation establishes a programmable therapeutic interface that coordinates electric‐field modulation, transient or irreversible membrane permeabilization, cargo stabilization, and immune remodeling. This Review highlights how nanocarriers, hydrogels, and bioelectronic interfaces expand electroporation from conventional ...
Binbin Ji, Zhuozhuo Wu, Yuyue Jiang, Xiaoyu Liu, Jingfeng Li, Fengkuo Xu, Chaojie Li, Qungang Shan, Ziyin Wang, Junhao Wang, Wei Huang, Yaping Zhuang, Wenguo Cui, Zhongmin Wang   +13 more
wiley   +1 more source

Endocytosis‐independent cytosolic entry of messenger RNA via fluorous bilayer zippering attenuating Toll‐like receptor signaling and enables ischemic tissue salvage

Smart Molecules, EarlyView.
The present study establishes a paradigm shift in synthetic mRNA delivery through the convergence of three orthogonal chemistries—perfluoro‐acylation, bio‐orthogonal click cross‐linking, and disulfide reduction—within a single, modular polymer architecture. Abstract A fundamental constraint of conventional messenger RNA (mRNA) delivery systems is their
Yue Wang, Haitao Xie, Guoqing Xiang, Yanhua Li, Changgui Tong, Hongyan Cui, Qixian Chen, Haidong Li, Yan Zhao   +8 more
wiley   +1 more source

Evaluation of Flavones Binding to Nucleic Acids through Competitive Dialysis [PDF]

Competitive dialysis is a method in which the selective binding of ligands to nucleic acids can be quantified. This method utilizes the concept of diffusion against a semipermeable membrane to evaluate concentrations of binding.
Jiang, Holly, Gu, Landy, Wu, Chenfei, Lin, Jiajun, Xue, Liang   +4 more
core   +2 more sources