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Development of an RT-LAMP-CRISPR/Cas12a-based nucleic acid detection platform for rapid diagnosis of porcine reproductive and respiratory syndrome virus type 2. [PDF]
Vet Anim SciLi C, Sun C, Xu Q.
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A spectroscopic and <i>in silico</i> study on ruthenium(ii) complexes bearing pyridine-carboxamide ligands interacting with DNA as potential therapeutics. [PDF]
RSC AdvSookai S +3 more
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Thermal denaturation of nucleic acids in polyacrylamide gels
Analytical Biochemistry, 1974Abstract A system is described for measuring thermal denaturation of nucleic acid fractions directly in polyacrylamide gels. Total nucleic acids were fractionated by disc gel electrophoresis. The buffer within the gel was then exchanged for one commonly used in denaturation studies.
Jonathan Gressel
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Nucleic acid denaturation at an electrode surface [PDF]
, 2011 This thesis describes the development and improvement of an assay for the detection and discrimination of DNA sequences based on surface enhanced Raman spectroscopy (SERS) and electrochemically driven denaturation. A DNA helix can be immobilised at a nano-structured gold electrode and then denatured into its constituent components by driving the ...
Johnson, Robert Peter
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Biochimica Et Biophysica Acta, 1960
Abstract Sedimentation studies indicate that native DNA and DNA denatured by dilution with water below the critical concentration zone have similar sedimentation coefficients and sedimentation coefficient concentration dependence. This is similar to the findings for alkali and thermally denatured DNA.
D O Jordan, R B Inman, D O Jordan
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Abstract Sedimentation studies indicate that native DNA and DNA denatured by dilution with water below the critical concentration zone have similar sedimentation coefficients and sedimentation coefficient concentration dependence. This is similar to the findings for alkali and thermally denatured DNA.
D O Jordan, R B Inman, D O Jordan
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The denaturation of desoxypentose nucleic acid
Biochimica Et Biophysica Acta, 1955Stuart Rice, Paul Doty
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The Thermal Denaturation of Desoxyribose Nucleic Acid
Journal of the American Chemical Society, 1957Stuart Rice, Paul Doty
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The Alkaline Denaturation of Deoxyribose Nucleic Acid
Journal of the American Chemical Society, 1958Paul Doty
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ChemBioChem, 2004
AbstractIntercalating nucleic acids (INA®s) with insertions of (R)‐1‐O‐(1‐pyrenylmethyl)glycerol were hybridized with locked nucleic acids (LNAs). INA/LNA duplexes were found to be less stable than the corresponding DNA/LNA duplexes when the INA monomer was inserted as a bulge close to the LNA monomers in the opposite strand.
Filichev, V.V. +5 more
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AbstractIntercalating nucleic acids (INA®s) with insertions of (R)‐1‐O‐(1‐pyrenylmethyl)glycerol were hybridized with locked nucleic acids (LNAs). INA/LNA duplexes were found to be less stable than the corresponding DNA/LNA duplexes when the INA monomer was inserted as a bulge close to the LNA monomers in the opposite strand.
Filichev, V.V. +5 more
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Analysis and purification of peptide nucleic acids by denaturing PAGE
ELECTROPHORESIS, 2007AbstractA flexible and convenient protocol for the analysis and purification of peptide nucleic acid (PNA) oligomers and PNA‐peptide chimeras by denaturing PAGE is described. Vertical slab gel electrophoresis, 26% in polyacrylamide and 8 M urea at pH 3, was suitable for analysis of oligomers ranging in size from tetramers (4‐mers) to tetradodecamers ...
David W, Dodd, Robert H E, Hudson
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