Results 151 to 160 of about 2,650,464 (200)
Some of the next articles are maybe not open access.
Numerical analysis of thermal denaturation of nucleic acids
Analytical Biochemistry, 1978Denaturation of DNA molecules by stepwise incrementation of the temperature leads to melting profiles showing a fine structure, composed of individual melting modes. A method is described by which quantitative physical information brought by the modes can be extracted from the melting profile.
openaire +2 more sources
Pyridine: a Denaturant or Stabilizer of Spherical Nucleic Acids?
Analytical Chemistry, 2017The spotlighted dual functions of pyridine as a denaturant and as a stabilizer for duplex DNA are thoroughly investigated using spherical nucleic acids (SNAs). At neutral pH, pyridine destabilizes the duplex interconnects of assembled SNAs, resulting in a gradual decrease in their melting temperature (Tm) as a function of the pyridine concentration ...
Yoon Hyuck Kim +3 more
openaire +2 more sources
Effects of cupric ions on thermal denaturation of nucleic acids
Journal of Molecular Biology, 1965The midpoint of thermal denaturation of sodium DNA and RNA decreases with increasing concentration of cupric ions. With Micrococcus lysodeikticus DNA, the Tm† is 69°C without cupric ions and 36°C with 0·1 m M -cupric ions in 5 m M -sodium chloride. In a solvent consisting of 5 m M -sodium chloride and 0·1 m M -cupric ions, Tm values of several DNA's ...
openaire +2 more sources
Small-volume cuvette for high-resolution thermal denaturation of nucleic acids
Analytical Biochemistry, 1979Abstract A small-volume cuvette and hoider makes it possible to obtain useful high-resolution thermal denaturation profiles on as little as submicrogram quantities of DNA and routine results with approximately 2 μg of DNA. The reduced sample size is especially important in the thermal characterization of DNA restriction fragments.
A T, Ansevin, D L, Vizard
openaire +2 more sources
Theoretical Aspects of Thermal Denaturation of Nucleic Acids
1998Information on mutations (as measured by D/TGGE) can be significantly improved by the use of a theoretical description of the thermal denaturation of nucleic acids.
Michael Etscheid, Detlev Riesner
openaire +1 more source
Microfluidic chip of fast DNA hybridization using denaturing and motion of nucleic acids
ELECTROPHORESIS, 2008AbstractThis study presents the effect of fluidic temperatures and velocities on improving DNA hybridization. The efficiency of hybridization could be improved by introducing elevated temperature in the hot region and velocity in the cold region. Compared with the conventional methods, this hybridization microchip was able to increase the hybridization
Yung-Chiang Chung +5 more
openaire +3 more sources
Chromatography of nucleic acids on hydroxyapatite II. Chromatography of denatured DNA
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1969Abstract The chromatographic behavior of DNA denatured by heat at various temperatures, and reacted or not with formaldehyde, on hydroxyapatite columns has been investigated using both stepwise and gradient elution. The recovery of denatured DNA from the columns and the behavior of the chromatographic fractions upon rechromatography have been studied.
openaire +2 more sources
Methods for thermal denaturation studies of nucleic acids in complex with fluorogenic dyes
2019Thermal denaturation is a common technique in the biophysical study of nucleic acids. These experiments are typically performed by monitoring the increase in absorbance (hyperchromism) of a sample at 260nm with temperature (Mergny & Lacroix, 2003; Puglisi & Tinoco, 1989).
Lauren M, Aufdembrink +5 more
openaire +2 more sources
2000
Publisher Summary This chapter describes the Monte Carlo applications to thermal and chemical denaturation experiments of nucleic acids and proteins. Information about the states of systems must often be extracted indirectly, from the measurements of properties, considered characteristic of these states.
D J, Williams, K B, Hall
openaire +2 more sources
Publisher Summary This chapter describes the Monte Carlo applications to thermal and chemical denaturation experiments of nucleic acids and proteins. Information about the states of systems must often be extracted indirectly, from the measurements of properties, considered characteristic of these states.
D J, Williams, K B, Hall
openaire +2 more sources
Exploring the folding and assembly of nucleic acids by thermal denaturation
2018Nucleic acids are highly dynamic biomolecules that regulate biological function and are widely used to construct nanoscale materials. Nucleic acid folding and assembly dynamics modulate how they interact with the environment to achieve specific molecular outcomes.
openaire +1 more source