Results 171 to 180 of about 146,288 (301)

High‐throughput multispecies quantitative polymerase chain reaction assays to study the effects of acute thermal stress in three species of Acipenser sturgeon

open access: yesJournal of Fish Biology, EarlyView.
Abstract Fishes experience thermal stress in their natural environment that can be caused by natural or anthropogenic factors and can directly affect their physiology. In this study, we developed a multispecies OpenArray™ qPCR (quantitative polymerase chain reaction) ‘chip’ to measure the effects of acute thermal stress on the mRNA response of ...
Hossein Haghighi   +6 more
wiley   +1 more source

A Formalin‐Inactivated Vaccine Enhances Survival and Mitigates Horizontal Transmission of Red Sea Bream Iridovirus (RSIV) in Rock Bream (Oplegnathus fasciatus): Insights From Viability Quantitative PCR

open access: yesJournal of Fish Diseases, EarlyView.
ABSTRACT Formalin‐inactivated vaccines are widely employed as a primary preventive strategy against red sea bream iridoviral disease (RSIVD), which poses a substantial economic threat to the aquaculture of rock bream (Oplegnathus fasciatus). However, conventional quantitative PCR (qPCR) cannot differentiate infectious virions from noninfectious vaccine
Sung‐Bin Moon   +8 more
wiley   +1 more source

Expanding the Fish‐Brain Invitrome With the Senegalese Sole SsB‐1 Cell Line—A Versatile Model for Neurotropic Virus Research

open access: yesJournal of Fish Diseases, EarlyView.
ABSTRACT Cell lines are essential tools for studying animal physiology and immunopathology, reducing the use of live animals and supporting the 3Rs principle of reduction, refinement, and replacement. The Senegalese sole (Solea senegalensis), a relevant species for Spanish aquaculture diversification, lacks established in vitro models. We developed SsB‐
Yulema Valero, Alberto Cuesta
wiley   +1 more source

The HaMYB22–HaGST3.2 module mediates salt stress response in sunflower

open access: yesJournal of Integrative Plant Biology, EarlyView.
The transcription factors HaMYB22, HaMYB120, and HaMYB121 cooperatively activate the transcription of the glutathione S‐transferase gene HaGST3.2, thereby facilitating the removal of reactive oxygen species induced by salt stress in sunflowers and improving their salt tolerance.
Siqi Zhang   +11 more
wiley   +1 more source

Transmission electron microscopy characterisation of Spirulina bioplastics

open access: yesJournal of Microscopy, EarlyView.
Abstract Plastic pollution is impacting global ecosystem health. Bioplastics made from unprocessed biomass, including whole or fragmented tissues, are one potential solution. They are biodegradable and exhibit competitive mechanical performance. Seaweed and microalgae have gained popularity over the last decade as bioplastic feedstocks due to their ...
Sourena Azidhak   +4 more
wiley   +1 more source

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