Results 81 to 90 of about 67,253 (293)

Membrane Damage and Metabolic Disruption as the Mechanisms of Linalool against Pseudomonas fragi: An Amino Acid Metabolomics Study

Foods
Pseudomonas fragi (P. fragi) is usually detected in low-temperature meat products, and seriously threatens food safety and human health. Therefore, the study investigated the antibacterial mechanism of linalool against P.
Jiaxin Cai, Haiming Chen, Runqiu Wang, Qiuping Zhong, Weijun Chen, Ming Zhang, Rongrong He, Wenxue Chen   +7 more
doaj   +1 more source

Comparison of different protocols for the bovine viral diarrhea virus detection by RT-PCR in pools of whole blood and blood serum artificially contaminated/
Comparação de diferentes protocolos para a detecção do vírus da diarréia viral bovina por RT-PCR em grupos de sangue total e de soro sangüíneo, artificialmente contaminados

Semina: Ciências Agrárias, 2005
The RT-PCR technique was optimized and evaluated to detect the 5’ untranslated region of bovine viral diarrhea virus (BVDV) from clinical samples that consisted of blood serum and whole blood artificially contaminated with the NADL strain of BVDV.
Amauri A. Alfieri, Alice F. Alfieri, Daniela Pilz   +2 more
doaj   +1 more source

Development of a loop‐mediated isothermal amplification‐lateral flow dipstick (LAMP‐LFD) assay and on‐site rapid detection evaluation for Rodentibacter heylii and Rodentibacter pneumotropicus

Animal Models and Experimental Medicine, EarlyView.
This study established a closed‐tube LAMP‐LFD assay for detecting Rodentibacter heylii and Rodentibacter pneumotropicus. The method exhibited high sensitivity (detection limits of 10−5 ng/μL for R. heylii and 10−4 ng/μL for R. pneumotropicus), high specificity, reliable performance with clinical samples, and effective validation in experimental ...
Huiqiong Yan, Sisi Chen, Yuhan Gan, Jing Xing, Yufang Feng, Xin Wen, Jie Fang, Jiangtao Du, Shasang Zhou, Honggang Guo, Xiaoyin Jin, Zhiyuan Wang, Junhao Tao, Lingqun Lu, Qingming Kong, Huazhong Ying, Wei Han, Fangwei Dai   +17 more
wiley   +1 more source

Antimicrobial Efficacy of Honey’s Flavonoids and Phenolic Acids: Implications for Wound Healing and Pharmaceutical Development

Wah Academia Journal of Health and Nutrition
The antimicrobial nature of honey has been appreciated for thousands of years; however, the molecular mechanism through which its function is only now being unraveled.
Faiqa Shakeel, Ihsan Ali Rind, Zara Ijaz, Mahnoor Dua Kotwal, Sanam Nawaz, Maria, Hiba Noor, Syeda Rubab Toqeer   +7 more
doaj   +1 more source

Tensile Stimulation in Biorelevant Culture Conditions Enhances MSC and TPSC Tenogenesis on Aligned Electrospun Scaffolds

Advanced NanoBiomed Research, EarlyView.
Biomimetic electrospun scaffold incorporating GDF‐7‐loaded mesoporous silica nanoparticles, combined with mechanical stimulation and physiological oxygen tension, guides tenogenic differentiation of mesenchymal stromal cell and tendon progenitor stem cell. This integrated approach enhances cell proliferation, matrix deposition, and tendon‐specific gene
Vera Citro, Aldo R. Boccaccini, Nicholas R. Forsyth, Tina P. Dale   +3 more
wiley   +1 more source

Oligopyrenotides: Abiotic, Polyanionic Oligomers with Nucleic Acid-like Structural Properties

, 2016
Oligopyrenotides, abiotic oligomers that exhibit significant structural analogies to the nucleic acids, are described. They are composed of achiral, phosphodiester-linked pyrene building blocks and a single chiral 1,2-diaminocyclohexane unit.
Florian Garo (317037), Daniel Wenger (2294923), Vladimir L. Malinovskii (2055145), Robert Häner (1486126)   +3 more
core   +1 more source

Denaturation and condensation of intracellular nucleic acids monitored by fluorescence depolarization of intercalating dyes in individual cells.

, 1989
The intercalating binding of planar aromatic dye molecules to nucleic acids can be analyzed using fluorescence depolarization measurements of the dye molecules excited by linearly polarized light. In this study, we investigated the conformational changes
Beisker, W., Eisert, W.G.
core   +2 more sources

Site‐Specific Disulfide‐Mediated Crosslinking of DNA Nanocubes for Enhanced Biological Applications

Small Science
The development of DNA nanotechnology has enabled the creation of diverse nanomaterials with significant potential in biological applications, such as sensing or drug delivery.
Sinan Faiad, Quentin Laurent, Jathavan Asohan, Tyler Brown, Alexander Prinzen, Hanadi Farouk Sleiman   +5 more
doaj   +1 more source

Enhanced-ice-COLD-PCR for the Sensitive Detection of Rare DNA Methylation Patterns in Liquid Biopsies

Bio-Protocol, 2019
In the context of precision medicine, the identification of novel biomarkers for the diagnosis of disease, prognosis, predicting treatment outcome and monitoring of treatment success is of great importance.
Florence Mauger, Jörg Tost
doaj   +1 more source

Putative DNA G-quadruplex formation within the promoters of Plasmodium falciparum var genes

BMC Genomics, 2009
Background Guanine-rich nucleic acid sequences are capable of folding into an intramolecular four-stranded structure called a G-quadruplex. When found in gene promoter regions, G-quadruplexes can downregulate gene expression, possibly by blocking the ...
Rowe J, Teulade-Fichou Marie-Paule, De Pauw Edwin, Rosu Frédéric, Damblon Christian, Gabelica Valérie, Smargiasso Nicolas, Claessens Antoine   +7 more
doaj   +1 more source