Results 81 to 90 of about 67,468 (297)

Versatile Detection of Cellular Protein via Fluorescence Anisotropy

Advanced Science, EarlyView.
Cell Lysate Fluorescence Anisotropy (CFAST) utilizes long lifetime dye‐labeled nanobodies for rapid protein quantification in minimally purified, complex cell lysate. When coupled with cellular thermal shift, CFAST allows high‐throughput detection of endogenous protein‐small molecule engagement, facilitating the discovery of novel binders for ...
Qing Tang, Yuan‐Ping Wei, Ricky Ruiqi Ma, Na Wei, Tammy Zihan Zhou, Hilary Kung‐Yu Ho, Zafar Iqbal Bhat, Iqra Ishrat, Xiaoyu Li, Judy Wai Ping Yam, Yung Hou Wong, Qiankun Wang, Justin L. Tan   +12 more
wiley   +1 more source

Use of the “Ru‐1O2‐Hydrazide” System Catalyzed by Metallic Ruthenium Complexes to Decipher the Interaction Between Microbes and Host Cancer Cells

Advanced Science, EarlyView.
ABSTRACT The dynamic interplay between bacteria and host cancer cells plays a critical role in tumor microenvironment modulation, bacterial pathogenesis, and potential oncotherapy applications. However, traditional methods often fail to capture transient or spatially restricted molecular interactions at the bacteria‐cancer cell interface.
Amin Sun, Kaihong Wang, Haifu Sun, Kemei Tao, Xiang Li, Xiuhua Zhao, Shuang Qiu   +6 more
wiley   +1 more source

Single Molecule Kinetic and Thermodynamic Studies of Molecular Crowding and Extreme Pressure Effects on Nucleic Acid Conformations [PDF]

, 2022
Biochemical activity of proteins and nucleic acids is strongly dependent on threedimensional shape. As a result, upon synthesis biomolecules often undergo a series of secondary and tertiary folding transitions to achieve a spatial configuration crucial ...
Sung, Hsuan-Lei
core   +3 more sources

Cerium Nanoparticle‐Mediated Inhibition of the NSUN2/m5C Axis Suppresses Synovial Aggression in Rheumatoid Arthritis

Advanced Science, EarlyView.
A novel epitranscriptomic mechanism in rheumatoid arthritis is uncovered: NSUN2 promotes disease via m5C‐dependent stabilization of ICMT mRNA, fueling the migration and invasion of pathogenic RA FLS. Targeting this axis with engineered nanoparticles (Ce/SAA NPs) effectively inhibits disease progression, presenting a precise therapeutic strategy ...
Ruiru Li, Simin Chen, Huijuan Hu, Shiyao Wu, Jingnan Wang, Kai Sun, Chenxi Peng, Suling Liu, Shuoyang Zhang, Yu Kuang, Wei Chen, Qian Qiu, Liuqin Liang, Yu Wen, Hanshi Xu, Youjun Xiao   +15 more
wiley   +1 more source

Development and performance evaluation of a streamlined method for nucleic acid purification, denaturation, and multiplex detection of \u3ci\u3eBluetongue virus\u3c/i\u3e and \u3ci\u3eEpizootic hemorrhagic disease virus\u3c/i\u3e [PDF]

, 2013
Bluetongue virus (BTV) and Epizootic hemorrhagic disease virus (EHDV) possess similar structural and molecular features, are transmitted by biting midges (genus Culicoides), and cause similar diseases in some susceptible ruminants.
Ostlund, Eileen N., Johnson, Donna J., Schroeder, Megan E., Bounpheng, Mangkey A., Meier, Jennifer, Clavijo, Alfonso   +5 more
core   +1 more source

DEOXYRIBONUCLEIC ACID CYTOPHOTOMETRY OF STAINED HUMAN LEUKOCYTES III. THERMAL DENATURATION OF CHROMATIN [PDF]

, 1974
Thermal denaturation of chromatin has been monitored in situ in single cells using absorption cytophotometry on gallocyanin-chrome alum (GCA)-stained preparations.
BRIAN H. MAYALL, STEPHEN C. DRESKIN
core   +1 more source

Membrane Damage and Metabolic Disruption as the Mechanisms of Linalool against Pseudomonas fragi: An Amino Acid Metabolomics Study

Foods
Pseudomonas fragi (P. fragi) is usually detected in low-temperature meat products, and seriously threatens food safety and human health. Therefore, the study investigated the antibacterial mechanism of linalool against P.
Jiaxin Cai, Haiming Chen, Runqiu Wang, Qiuping Zhong, Weijun Chen, Ming Zhang, Rongrong He, Wenxue Chen   +7 more
doaj   +1 more source

Evaluation of the impact of six different DNA extraction methods for the representation of the microbial community associated with human chronic wound infections using a gel-based DNA profiling method

AMB Express, 2017
Infected chronic wounds are polymicrobial in nature which include a diverse group of aerobic and anaerobic microorganisms. Majority of these communal microorganisms are difficult to grow in vitro.
Ayomi Dilhari, Asanga Sampath, Chinthika Gunasekara, Neluka Fernando, Deepaka Weerasekara, Chris Sissons, Andrew McBain, Manjula Weerasekera   +7 more
doaj   +1 more source

Controlled Peptide Capture and Release in 3D‐Printed Multimaterial Microstructures

Advanced Science, EarlyView.
Two‐photon laser printing of 3D microstructures using a peptide‐containing ink is shown. The microstructures allow for controlled capture and release of target peptides through coiled‐coil interaction with the printed peptide inside the material. Based on these properties, orthogonal capture and stimuli‐mediated release possibilities inside printed ...
Niklas Schwegler, Thomas Heim, Philipp Mainik, Eva Blasco, Franziska Thomas   +4 more
wiley   +1 more source

Lab-on-a-chip systems for the analysis of phytoplankton RNA [PDF]

, 2013
Monitoring microorganisms in natural water is central to understanding and managing risks to human health and ecosystems. Some phytoplankton can produce toxic blooms which are harmful to aquatic ecosystems and human health.
Bahi, Mahadji
core