Results 281 to 290 of about 323,426 (336)

Spherical Nucleic Acid Probes on Floating-Gate Field-Effect Transistor Biosensors for Attomolar-Level Analyte Detection.

ACS Nano
Field-effect transistor (FET) sensors are attractive for the label-free detection of target biomolecules, offering ultrahigh sensitivity and a rapid response.
Haoran Wang, Jing Xie, Mengmeng Xiao, Yuehua Ke, Jiawang Li, Zongyu Nie, Qiaoshu Chen, Zhiyong Zhang   +7 more
semanticscholar   +1 more source

Counterion association with native and denatured nucleic acids: an experimental approach 1 1Edited by I. Tinoco

Journal of Molecular Biology, 2001
The melting temperature of the poly(dA) . poly(dT) double helix is exquisitely sensitive to salt concentration, and the helix-to-coil transition is sharp. Modern calorimetric instrumentation allows this transition to be detected and characterized with high precision at extremely low duplex concentrations.
Jens Völker, Gerald S. Manning, Horst H. Klump, Kenneth J. Breslauer   +3 more
openaire   +3 more sources

Fast DNA hybridization chip using denature and motion of nucleic acids

2010 5th International Microsystems Packaging Assembly and Circuits Technology Conference, 2010
We demonstrate that the efficiency of DNA hybridization could be improved by introducing elevated temperature in the hot region and higher velocities in the cold region of a microfluidic chip. Compared with the conventional methods, this hybridization microchip was shown to increase the hybridization signal 4.6-fold within 30 minutes using a 1.4 kb ...
Yu-Cheng Lin, Chaung-Di Chueh, Wei-Chieh Liao, Chuan-You Ye, Yung-Chiang Chung, Li-Wei Lai   +5 more
openaire   +2 more sources

Chromatography of nucleic acids on hydroxyapatite II. Chromatography of denatured DNA

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1969
Abstract The chromatographic behavior of DNA denatured by heat at various temperatures, and reacted or not with formaldehyde, on hydroxyapatite columns has been investigated using both stepwise and gradient elution. The recovery of denatured DNA from the columns and the behavior of the chromatographic fractions upon rechromatography have been studied.
openaire   +3 more sources

Structural Analysis of Nucleic Acids by Precise Denaturing Gradient Gel Electrophoresis: I. Methodology1

The Journal of Biochemistry, 1992
A method to convert the conventional denaturing gradient gel electrophoresis into a highly reproducible experimental system was developed. It was based on the following experimental findings; (i) dyes, which are small molecules, do not exhibit mobility changes attributed to their conformational change while nucleic acids do; and (ii) most of the ...
Misa Tsubota, Yuzuru Husimi, Yasuhiro Chonan, Toshihisa Miura, Koichi Nishigaki   +4 more
openaire   +3 more sources

[4] Use of denaturing gradient gel electrophoresis to study conformational transitions in nucleic acids

1992
Publisher Summary This chapter presents the study of conformational transitions in nucleic acids using denaturing gradient gel electrophoresis (DGGE). The term melting is used to denote, for double-stranded DNA (dsDNA), the temperature- or solvent-induced transition from a double-stranded, helical conformation to a single-stranded, random coil ...
Ezra S. Abrams, Vincent P. Stanton
openaire   +3 more sources

Monitoring the progression of the in vitro selection of nucleic acid aptamers by denaturing high-performance liquid chromatography

Analytical and Bioanalytical Chemistry, 2007
Monitoring of in-vitro selection experiments is crucial in evaluation of the success and outcome of such approaches. Furthermore, monitoring running in parallel with the selection procedure enables early intervention and adjustment of stringency to achieve the desired activities of the selected nucleic acid species.
Müller, Jens, El-Maarri, Osman, Oldenburg, Johannes, Pötzsch, Bernd, Mayer, G.   +4 more
openaire   +4 more sources

Influence of adsorbability of denatured deoxyribonucleic acid on its polarographic reducibility

Journal of Electroanalytical Chemistry, 1974
Summary The polarographic reduction of denatured DNA takes place in the adsorbed state. Reduction polarographic currents of denatured DNA in those pH regions, where the current does not change much with pH, have the character of adsorption currents under conditions when the surface of mercury electrode is fully covered by adsorbed polynucleotide ...
openaire   +2 more sources

Integrated silica membrane–based nucleic acid purification, amplification, and visualization platform for low-cost, rapid detection of foodborne pathogens

Analytical and Bioanalytical Chemistry, 2020
Xiudan Wang, Chunyu Yan, Xiaokun Wang, Xiaoli Zhao, Chao Shi, Cuiping Ma   +5 more
semanticscholar   +1 more source

[Alternating current polarography criteria of nucleic acid denaturation].

Biopolymers, 1967
H. Berg, H. Bär, F. Gollmick
semanticscholar   +1 more source