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Biochemistry and Molecular Biology Education, 2020
AbstractThe Förster resonance energy transfer (FRET) melting assay intends to evaluate the unfolding, denaturation process of DNA secondary structures, and its stabilization using compounds known as DNA binders, some of which are highly specific for G‐quadruplex DNAs versus duplex DNAs.
Josué Carvalho, Carla Cruz
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AbstractThe Förster resonance energy transfer (FRET) melting assay intends to evaluate the unfolding, denaturation process of DNA secondary structures, and its stabilization using compounds known as DNA binders, some of which are highly specific for G‐quadruplex DNAs versus duplex DNAs.
Josué Carvalho, Carla Cruz
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Chromatography of nucleic acids on hydroxyapatite II. Chromatography of denatured DNA
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1969Abstract The chromatographic behavior of DNA denatured by heat at various temperatures, and reacted or not with formaldehyde, on hydroxyapatite columns has been investigated using both stepwise and gradient elution. The recovery of denatured DNA from the columns and the behavior of the chromatographic fractions upon rechromatography have been studied.
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Methods for thermal denaturation studies of nucleic acids in complex with fluorogenic dyes
2019Thermal denaturation is a common technique in the biophysical study of nucleic acids. These experiments are typically performed by monitoring the increase in absorbance (hyperchromism) of a sample at 260nm with temperature (Mergny & Lacroix, 2003; Puglisi & Tinoco, 1989).
Lauren M, Aufdembrink +5 more
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2000
Publisher Summary This chapter describes the Monte Carlo applications to thermal and chemical denaturation experiments of nucleic acids and proteins. Information about the states of systems must often be extracted indirectly, from the measurements of properties, considered characteristic of these states.
D J, Williams, K B, Hall
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Publisher Summary This chapter describes the Monte Carlo applications to thermal and chemical denaturation experiments of nucleic acids and proteins. Information about the states of systems must often be extracted indirectly, from the measurements of properties, considered characteristic of these states.
D J, Williams, K B, Hall
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2015 40th International Conference on Infrared, Millimeter, and Terahertz waves (IRMMW-THz), 2015
Dynamics and structure of water network around nucleic acid have been subjects of intensive studies due to their contributions to biological process. Here, we identified spatiotemporal features of DNA hydration through examining fully-hydrated DNA solutions by THz spectroscopy. The results suggest the presence of weakly bound water beyond tight-binding
Heyjin Son +7 more
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Dynamics and structure of water network around nucleic acid have been subjects of intensive studies due to their contributions to biological process. Here, we identified spatiotemporal features of DNA hydration through examining fully-hydrated DNA solutions by THz spectroscopy. The results suggest the presence of weakly bound water beyond tight-binding
Heyjin Son +7 more
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Journal of Biomolecular Structure and Dynamics, 1984
At high binding densities acridine orange (AO) forms complexes with ds DNA which are insoluble in aqueous media. These complexes are characterized by high red- and minimal green-luminescence, 1:1 (dye/P) stoichiometry and resemble complexes of AO with ss nucleic acids.
J, Kapuscinski, Z, Darzynkiewicz
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At high binding densities acridine orange (AO) forms complexes with ds DNA which are insoluble in aqueous media. These complexes are characterized by high red- and minimal green-luminescence, 1:1 (dye/P) stoichiometry and resemble complexes of AO with ss nucleic acids.
J, Kapuscinski, Z, Darzynkiewicz
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Counterion association with native and denatured nucleic acids: an experimental approach.
Journal of molecular biology, 2001The melting temperature of the poly(dA) . poly(dT) double helix is exquisitely sensitive to salt concentration, and the helix-to-coil transition is sharp. Modern calorimetric instrumentation allows this transition to be detected and characterized with high precision at extremely low duplex concentrations.
J, Völker +3 more
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The denaturation of desoxypentose nucleic acid
Biochimica et Biophysica Acta, 1955P, DOTY, S A, RICE
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Influence of adsorbability of denatured deoxyribonucleic acid on its polarographic reducibility
Journal of Electroanalytical Chemistry, 1974Summary The polarographic reduction of denatured DNA takes place in the adsorbed state. Reduction polarographic currents of denatured DNA in those pH regions, where the current does not change much with pH, have the character of adsorption currents under conditions when the surface of mercury electrode is fully covered by adsorbed polynucleotide ...
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Journal of biochemistry, 1992
A method to convert the conventional denaturing gradient gel electrophoresis into a highly reproducible experimental system was developed. It was based on the following experimental findings; (i) dyes, which are small molecules, do not exhibit mobility changes attributed to their conformational change while nucleic acids do; and (ii) most of the ...
K, Nishigaki +4 more
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A method to convert the conventional denaturing gradient gel electrophoresis into a highly reproducible experimental system was developed. It was based on the following experimental findings; (i) dyes, which are small molecules, do not exhibit mobility changes attributed to their conformational change while nucleic acids do; and (ii) most of the ...
K, Nishigaki +4 more
openaire +1 more source