Results 261 to 270 of about 67,468 (297)

Chromatography of nucleic acids on hydroxyapatite II. Chromatography of denatured DNA

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1969
Abstract The chromatographic behavior of DNA denatured by heat at various temperatures, and reacted or not with formaldehyde, on hydroxyapatite columns has been investigated using both stepwise and gradient elution. The recovery of denatured DNA from the columns and the behavior of the chromatographic fractions upon rechromatography have been studied.
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Monte Carlo applications to thermal and chemical denaturation experiments of nucleic acids and proteins

2000
Publisher Summary This chapter describes the Monte Carlo applications to thermal and chemical denaturation experiments of nucleic acids and proteins. Information about the states of systems must often be extracted indirectly, from the measurements of properties, considered characteristic of these states.
D J, Williams, K B, Hall
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Monitoring the progression of the in vitro selection of nucleic acid aptamers by denaturing high-performance liquid chromatography

Analytical and Bioanalytical Chemistry, 2007
Monitoring of in-vitro selection experiments is crucial in evaluation of the success and outcome of such approaches. Furthermore, monitoring running in parallel with the selection procedure enables early intervention and adjustment of stringency to achieve the desired activities of the selected nucleic acid species.
Müller, Jens, El-Maarri, Osman, Oldenburg, Johannes, Pötzsch, Bernd, Mayer, G.   +4 more
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Influence of adsorbability of denatured deoxyribonucleic acid on its polarographic reducibility

Journal of Electroanalytical Chemistry, 1974
Summary The polarographic reduction of denatured DNA takes place in the adsorbed state. Reduction polarographic currents of denatured DNA in those pH regions, where the current does not change much with pH, have the character of adsorption currents under conditions when the surface of mercury electrode is fully covered by adsorbed polynucleotide ...
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Counterion association with native and denatured nucleic acids: an experimental approach.

Journal of molecular biology, 2001
The melting temperature of the poly(dA) . poly(dT) double helix is exquisitely sensitive to salt concentration, and the helix-to-coil transition is sharp. Modern calorimetric instrumentation allows this transition to be detected and characterized with high precision at extremely low duplex concentrations.
J, Völker, H H, Klump, G S, Manning, K J, Breslauer   +3 more
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Structural analysis of nucleic acids by precise denaturing gradient gel electrophoresis: I. Methodology.

Journal of biochemistry, 1992
A method to convert the conventional denaturing gradient gel electrophoresis into a highly reproducible experimental system was developed. It was based on the following experimental findings; (i) dyes, which are small molecules, do not exhibit mobility changes attributed to their conformational change while nucleic acids do; and (ii) most of the ...
K, Nishigaki, M, Tsubota, T, Miura, Y, Chonan, Y, Husimi   +4 more
openaire   +1 more source

On the conformation of native and denatured nucleic acids

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1966
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The Electrophoresis of Native and Denatured Nucleic Acids

2020
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Nucleic Acid Tests for Clinical Translation

Chemical Reviews, 2021
Fan Li, Qiang Xia, Xiaolei Zuo
exaly  

Direct, sequence-specific detection of dsDNA based on peptide nucleic acid and graphene oxide without requiring denaturation

Biosensors and Bioelectronics, 2014
Dal-Hee Min, Younghoon Lee, Jieon Lee
exaly