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Numerical analysis of thermal denaturation of nucleic acids
Analytical Biochemistry, 1978Denaturation of DNA molecules by stepwise incrementation of the temperature leads to melting profiles showing a fine structure, composed of individual melting modes. A method is described by which quantitative physical information brought by the modes can be extracted from the melting profile.
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Small-volume cuvette for high-resolution thermal denaturation of nucleic acids
Analytical Biochemistry, 1979Abstract A small-volume cuvette and hoider makes it possible to obtain useful high-resolution thermal denaturation profiles on as little as submicrogram quantities of DNA and routine results with approximately 2 μg of DNA. The reduced sample size is especially important in the thermal characterization of DNA restriction fragments.
A T, Ansevin, D L, Vizard
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Denaturation bubble-mediated two-stage isothermal nucleic acid amplification in a single closed tube
Chemical Communications, 2023A two-stage isothermal amplification method termed rolling cycle strand exchange amplification (RC-SEA) was established to accomplish ssRNA and dsDNA analysis using only one DNA polymerase in a single closed tube at a constant temperature.
Jinling Guo +7 more
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Microfluidic chip of fast DNA hybridization using denaturing and motion of nucleic acids
ELECTROPHORESIS, 2008AbstractThis study presents the effect of fluidic temperatures and velocities on improving DNA hybridization. The efficiency of hybridization could be improved by introducing elevated temperature in the hot region and velocity in the cold region. Compared with the conventional methods, this hybridization microchip was able to increase the hybridization
Yung-Chiang Chung +5 more
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Effects of cupric ions on thermal denaturation of nucleic acids
Journal of Molecular Biology, 1965The midpoint of thermal denaturation of sodium DNA and RNA decreases with increasing concentration of cupric ions. With Micrococcus lysodeikticus DNA, the Tm† is 69°C without cupric ions and 36°C with 0·1 m M -cupric ions in 5 m M -sodium chloride. In a solvent consisting of 5 m M -sodium chloride and 0·1 m M -cupric ions, Tm values of several DNA's ...
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Theoretical Aspects of Thermal Denaturation of Nucleic Acids
1998Information on mutations (as measured by D/TGGE) can be significantly improved by the use of a theoretical description of the thermal denaturation of nucleic acids.
Michael Etscheid, Detlev Riesner
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Fast DNA hybridization chip using denature and motion of nucleic acids
2010 5th International Microsystems Packaging Assembly and Circuits Technology Conference, 2010We demonstrate that the efficiency of DNA hybridization could be improved by introducing elevated temperature in the hot region and higher velocities in the cold region of a microfluidic chip. Compared with the conventional methods, this hybridization microchip was shown to increase the hybridization signal 4.6-fold within 30 minutes using a 1.4 kb ...
Yung-Chiang Chung +5 more
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Chromatography of nucleic acids on hydroxyapatite II. Chromatography of denatured DNA
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1969Abstract The chromatographic behavior of DNA denatured by heat at various temperatures, and reacted or not with formaldehyde, on hydroxyapatite columns has been investigated using both stepwise and gradient elution. The recovery of denatured DNA from the columns and the behavior of the chromatographic fractions upon rechromatography have been studied.
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2000
Publisher Summary This chapter describes the Monte Carlo applications to thermal and chemical denaturation experiments of nucleic acids and proteins. Information about the states of systems must often be extracted indirectly, from the measurements of properties, considered characteristic of these states.
D J, Williams, K B, Hall
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Publisher Summary This chapter describes the Monte Carlo applications to thermal and chemical denaturation experiments of nucleic acids and proteins. Information about the states of systems must often be extracted indirectly, from the measurements of properties, considered characteristic of these states.
D J, Williams, K B, Hall
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Analytical and Bioanalytical Chemistry, 2007
Monitoring of in-vitro selection experiments is crucial in evaluation of the success and outcome of such approaches. Furthermore, monitoring running in parallel with the selection procedure enables early intervention and adjustment of stringency to achieve the desired activities of the selected nucleic acid species.
Müller, Jens +4 more
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Monitoring of in-vitro selection experiments is crucial in evaluation of the success and outcome of such approaches. Furthermore, monitoring running in parallel with the selection procedure enables early intervention and adjustment of stringency to achieve the desired activities of the selected nucleic acid species.
Müller, Jens +4 more
openaire +4 more sources