Results 241 to 250 of about 67,253 (293)
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Biochimica Et Biophysica Acta, 1960
Abstract Sedimentation studies indicate that native DNA and DNA denatured by dilution with water below the critical concentration zone have similar sedimentation coefficients and sedimentation coefficient concentration dependence. This is similar to the findings for alkali and thermally denatured DNA.
D O Jordan
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Abstract Sedimentation studies indicate that native DNA and DNA denatured by dilution with water below the critical concentration zone have similar sedimentation coefficients and sedimentation coefficient concentration dependence. This is similar to the findings for alkali and thermally denatured DNA.
D O Jordan
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Methods for thermal denaturation studies of nucleic acids in complex with fluorogenic dyes
Methods in Enzymology, 2019Thermal denaturation is a common technique in the biophysical study of nucleic acids. These experiments are typically performed by monitoring the increase in absorbance (hyperchromism) of a sample at 260nm with temperature (Mergny & Lacroix, 2003; Puglisi & Tinoco, 1989).
Lauren M Aufdembrink +2 more
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Nucleic acid denaturation at an electrode surface [PDF]
, 2011 This thesis describes the development and improvement of an assay for the detection and discrimination of DNA sequences based on surface enhanced Raman spectroscopy (SERS) and electrochemically driven denaturation. A DNA helix can be immobilised at a nano-structured gold electrode and then denatured into its constituent components by driving the ...
Johnson, Robert Peter +1 more
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2015 40th International Conference on Infrared, Millimeter, and Terahertz waves (IRMMW-THz), 2015
Dynamics and structure of water network around nucleic acid have been subjects of intensive studies due to their contributions to biological process. Here, we identified spatiotemporal features of DNA hydration through examining fully-hydrated DNA solutions by THz spectroscopy. The results suggest the presence of weakly bound water beyond tight-binding
Heyjin Son +2 more
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Dynamics and structure of water network around nucleic acid have been subjects of intensive studies due to their contributions to biological process. Here, we identified spatiotemporal features of DNA hydration through examining fully-hydrated DNA solutions by THz spectroscopy. The results suggest the presence of weakly bound water beyond tight-binding
Heyjin Son +2 more
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The denaturation of desoxypentose nucleic acid
Biochimica Et Biophysica Acta, 1955Paul Doty, Stuart A Rice
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The Thermal Denaturation of Desoxyribose Nucleic Acid
Journal of the American Chemical Society, 1957Stuart A Rice, P Doty
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The Alkaline Denaturation of Deoxyribose Nucleic Acid
Journal of the American Chemical Society, 1958Paul Ehrlich, P Doty
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ChemBioChem, 2004
AbstractIntercalating nucleic acids (INA®s) with insertions of (R)‐1‐O‐(1‐pyrenylmethyl)glycerol were hybridized with locked nucleic acids (LNAs). INA/LNA duplexes were found to be less stable than the corresponding DNA/LNA duplexes when the INA monomer was inserted as a bulge close to the LNA monomers in the opposite strand.
Filichev, V.V. +5 more
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AbstractIntercalating nucleic acids (INA®s) with insertions of (R)‐1‐O‐(1‐pyrenylmethyl)glycerol were hybridized with locked nucleic acids (LNAs). INA/LNA duplexes were found to be less stable than the corresponding DNA/LNA duplexes when the INA monomer was inserted as a bulge close to the LNA monomers in the opposite strand.
Filichev, V.V. +5 more
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Analysis and purification of peptide nucleic acids by denaturing PAGE
ELECTROPHORESIS, 2007AbstractA flexible and convenient protocol for the analysis and purification of peptide nucleic acid (PNA) oligomers and PNA‐peptide chimeras by denaturing PAGE is described. Vertical slab gel electrophoresis, 26% in polyacrylamide and 8 M urea at pH 3, was suitable for analysis of oligomers ranging in size from tetramers (4‐mers) to tetradodecamers ...
David W, Dodd, Robert H E, Hudson
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Pyridine: a Denaturant or Stabilizer of Spherical Nucleic Acids?
Analytical Chemistry, 2017The spotlighted dual functions of pyridine as a denaturant and as a stabilizer for duplex DNA are thoroughly investigated using spherical nucleic acids (SNAs). At neutral pH, pyridine destabilizes the duplex interconnects of assembled SNAs, resulting in a gradual decrease in their melting temperature (Tm) as a function of the pyridine concentration ...
Yoon Hyuck Kim +3 more
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