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Hybridization of Nucleic Acids to Chromosomes
1972Ever since the method of molecular hybridization of nucleic acids was conceived, a number of biologists have tried to hybridize labeled RNA or DNA to chromosomes for detection by autoradiography. The first report was by French and Kitzmiller (1967), who hybridized 3H-DNA from Drosophila melanogaster to the DNA of the salivary chromosomes of the same ...
D M, Steffensen, D E, Wimber
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Microfluidic chip for fast nucleic acid hybridization
Lab on a Chip, 2003The design and experimental verification of a fast nucleic acid hybridization microchip using the fluidic velocity and strain rate effects was conducted. This hybridization chip was able to increase the hybridization signal 6-fold, reduce non-specific target-probe binding and background noise within 30 min, as compared to conventional hybridization ...
Yung-Chiang, Chung +3 more
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Nucleic Acid Hybridization: Triplex Stability and Energetics
Annual Review of Biophysics and Biomolecular Structure, 1995In this chapter, we review the current state of the thermodynamic database for triple helical oligonucleotide hybridization reactions and present a critical assessment of the methods used to obtain the relevant data. The thermodynamic stability of triple-helix oligonucleotide constructs is discussed in terms of its dependence on temperature, chain ...
G E, Plum +3 more
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Nucleic Acid Hybridization Procedures
2019The application of nucleic acid probes and related techniques is becoming common in the detection of human and other animal viruses but has lagged somewhat in plant virology. The explosion in the use of the technology associated with recombinant DNA research has led to the development of various techniques by which viruses can be diagnosed.
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Hybridization of Nucleic Acids
1969I am not sure there is a true difference in DNA-DNA hybridization between DNA from young and old animals. This is a complicated question because of difficulties in preparing DNA completely free of protein, the great complexity of mammalian DNA compared to bacterial DNA, and the greater probability for non specific inter an intra molecular interaction ...
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Identification of foodborne pathogens by nucleic acid hybridization
International Journal of Food Microbiology, 1991Nucleic acid hybridization methods have been developed and used to identify microorganisms in foods. Tests performed on mixed cultures save the time required to establish pure cultures. Enterotoxigenic or invasive strains of foodborne bacterial pathogens are detected with probes that identify genes responsible for virulence.
W E, Hill, S P, Keasler
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Nucleic Acid Hybridization to Isolated Chromatin
1972Three procedures are in general use for the formation and detection of RNA-DNA hybrids: reactions on a filter (Gillespie and Spiegelman, 1965); reaction in liquid (Nygaard and Hall, 1963); and reaction in agar (McCarthy and Bolton, 1964). The inherent limitations of each procedure have been studied in detail (Kennell and Kotoulas, 1968). Because of its
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Nucleic Acid Sandwich Hybridization in Adenovirus Diagnosis
1983The usefulness of viral diagnosis is critically dependent on the rapidity by which it is able to detect and identify virus taken from the site of infection. The methods suitable for rapid diagnosis so far available are mostly based on immunological detection of the virus and its antigenic components (for a review, see Halonen et al., this volume).
M, Ranki +7 more
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HYBRIDIZATION AND RENATURATION KINETICS OF NUCLEIC ACIDS
Annual Review of Biophysics and Bioengineering, 1976When two DNA or RNA molecules contain sequences of nucleotides that are complementary to one another, they are able to come together to form a double helix with all of the bases paired. If both strands are DNA, the process is usually called renaturation or reassociation; if one strand is DNA and the other is RNA, the process is called hybridization ...
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