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Simplified Identification of mRNA or DNA in Whole Cells [PDF]
A recently invented method of detecting a selected messenger ribonucleic acid (mRNA) or deoxyribonucleic acid (DNA) sequence offers two important advantages over prior such methods: it is simpler and can be implemented by means of compact equipment.
Almeida, Eduardo, Kadambi, Geeta
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ISOLATION AND PROPERTIES OF A NUCLEIC ACID HYBRID POLYMERASE [PDF]
Liebe F. Cavalieri, Sylvia Lee-Huang
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Hybridization of Peptide Nucleic Acid
Biochemistry, 1998The thermodynamics of hybridization and the conformations of decameric mixed purine-pyrimidine sequence PNA/PNA, PNA/DNA, and DNA/DNA duplexes have been studied using fluorescence energy transfer (FET), absorption hypochromicity (ABS), isothermal titration calorimetry (ITC), and circular dichroism (CD) techniques.
Ratilainen, T.+6 more
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In situ hybridization of nucleic acids [PDF]
Hybridization of radioactive nucleic acid sequences can be carried out with DNA in cytological preparations. By autoradiography distinct DNA sequences can then be localized in eukaryotic chromosomes.
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In situ nucleic acid hybridization
Survey of Ophthalmology, 1989Nucleic acid hybridization is a recently developed laboratory technique that allows identification of the genetic material in tissue specimens. The role of the nucleic acids DNA and RNA in cellular function and disease is reviewed, followed by discussion of nucleic acid detection techniques.
William R. Freeman, Clayton A. Wiley
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Labelling nucleic acids for hybridization
Nature, 1987The enormous utility of labelled nucleic acid probes has encouraged the development, over the past decade, of many efficient and reliable labelling methods.
C. R. Mundy, M. W. Cunningham
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Identification of foodborne pathogens by nucleic acid hybridization [PDF]
Nucleic acid hybridization methods have been developed and used to identify microorganisms in foods. Tests performed on mixed cultures save the time required to establish pure cultures. Enterotoxigenic or invasive strains of foodborne bacterial pathogens are detected with probes that identify genes responsible for virulence.
Walter E. Hill, Stacye P. Keasler
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ENVIRONMENTAL APPLICATION OF NUCLEIC ACID HYBRIDIZATION
Annual Review of Microbiology, 1990INTRODUCTION 625 NUCLEIC ACID HYBRIDIZATION PROBE TECHNOLOGy . . . . . . . . . . . . .. , .. "" ... "... 627 PROBE LABELLING AND HYBRID DETECTION."" .. " , 629 Radioactive Methods . . . . ... . ... . . . . . . . . . . . . .. . .. . . . . . . . . . . . . . . . , 629 NOllradioactive Methods . ,. .. . . . . . . . . . .. . . . . . . . .. . . . .. . . .
Alice C. Layton, Gary S. Sayler
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Hybridization of Nucleic Acids to Chromosomes
1972Ever since the method of molecular hybridization of nucleic acids was conceived, a number of biologists have tried to hybridize labeled RNA or DNA to chromosomes for detection by autoradiography. The first report was by French and Kitzmiller (1967), who hybridized 3H-DNA from Drosophila melanogaster to the DNA of the salivary chromosomes of the same ...
Dale M. Steffensen, D. E. Wimber
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2002
Nucleic acid hybridization allows the identification of homologous DNA and RNA sequences. This technique is used for gene mapping, gene expression studies and analysis of genomic organization.
Chan, Sarah, Choo, K H A
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Nucleic acid hybridization allows the identification of homologous DNA and RNA sequences. This technique is used for gene mapping, gene expression studies and analysis of genomic organization.
Chan, Sarah, Choo, K H A
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