Results 51 to 60 of about 110,082 (88)

Simplified Identification of mRNA or DNA in Whole Cells [PDF]

open access: yes
A recently invented method of detecting a selected messenger ribonucleic acid (mRNA) or deoxyribonucleic acid (DNA) sequence offers two important advantages over prior such methods: it is simpler and can be implemented by means of compact equipment.
Almeida, Eduardo, Kadambi, Geeta
core   +1 more source

ISOLATION AND PROPERTIES OF A NUCLEIC ACID HYBRID POLYMERASE [PDF]

open access: yesProceedings of the National Academy of Sciences, 1964
Liebe F. Cavalieri, Sylvia Lee-Huang
openaire   +3 more sources
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Hybridization of Peptide Nucleic Acid

Biochemistry, 1998
The thermodynamics of hybridization and the conformations of decameric mixed purine-pyrimidine sequence PNA/PNA, PNA/DNA, and DNA/DNA duplexes have been studied using fluorescence energy transfer (FET), absorption hypochromicity (ABS), isothermal titration calorimetry (ITC), and circular dichroism (CD) techniques.
Ratilainen, T.   +6 more
openaire   +3 more sources

In situ hybridization of nucleic acids [PDF]

open access: possibleTrends in Biochemical Sciences, 1976
Hybridization of radioactive nucleic acid sequences can be carried out with DNA in cytological preparations. By autoradiography distinct DNA sequences can then be localized in eukaryotic chromosomes.
openaire   +2 more sources

In situ nucleic acid hybridization

Survey of Ophthalmology, 1989
Nucleic acid hybridization is a recently developed laboratory technique that allows identification of the genetic material in tissue specimens. The role of the nucleic acids DNA and RNA in cellular function and disease is reviewed, followed by discussion of nucleic acid detection techniques.
William R. Freeman, Clayton A. Wiley
openaire   +3 more sources

Labelling nucleic acids for hybridization

Nature, 1987
The enormous utility of labelled nucleic acid probes has encouraged the development, over the past decade, of many efficient and reliable labelling methods.
C. R. Mundy, M. W. Cunningham
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Identification of foodborne pathogens by nucleic acid hybridization [PDF]

open access: possibleInternational Journal of Food Microbiology, 1991
Nucleic acid hybridization methods have been developed and used to identify microorganisms in foods. Tests performed on mixed cultures save the time required to establish pure cultures. Enterotoxigenic or invasive strains of foodborne bacterial pathogens are detected with probes that identify genes responsible for virulence.
Walter E. Hill, Stacye P. Keasler
openaire   +2 more sources

ENVIRONMENTAL APPLICATION OF NUCLEIC ACID HYBRIDIZATION

Annual Review of Microbiology, 1990
INTRODUCTION 625 NUCLEIC ACID HYBRIDIZATION PROBE TECHNOLOGy . . . . . . . . . . . . .. , .. "" ... "... 627 PROBE LABELLING AND HYBRID DETECTION."" .. " , 629 Radioactive Methods . . . . ... . ... . . . . . . . . . . . . .. . .. . . . . . . . . . . . . . . . , 629 NOllradioactive Methods . ,. .. . . . . . . . . . .. . . . . . . . .. . . . .. . . .
Alice C. Layton, Gary S. Sayler
openaire   +3 more sources

Hybridization of Nucleic Acids to Chromosomes

1972
Ever since the method of molecular hybridization of nucleic acids was conceived, a number of biologists have tried to hybridize labeled RNA or DNA to chromosomes for detection by autoradiography. The first report was by French and Kitzmiller (1967), who hybridized 3H-DNA from Drosophila melanogaster to the DNA of the salivary chromosomes of the same ...
Dale M. Steffensen, D. E. Wimber
openaire   +3 more sources

nucleic acid hybridization

2002
Nucleic acid hybridization allows the identification of homologous DNA and RNA sequences. This technique is used for gene mapping, gene expression studies and analysis of genomic organization.
Chan, Sarah, Choo, K H A
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