Results 61 to 70 of about 225,030 (341)

Cationic Magnetite Nanoparticles for Increasing siRNA Hybridization Rates

Nanomaterials, 2020
An investigation of the interaction principles of nucleic acids and nanoparticles is a priority for the development of theoretical and methodological approaches to creating bionanocomposite structures, which determines the area and boundaries of ...
Artur Y. Prilepskii, Arseniy Y. Kalnin, Anna F. Fakhardo, Elizaveta I. Anastasova, Daria D. Nedorezova, Grigorii A. Antonov, Vladimir V. Vinogradov   +6 more
doaj   +1 more source

Secondary structure formation of homopolymeric single-stranded nucleic acids including force and loop entropy: implications for DNA hybridization

, 2011
Loops are essential secondary structure elements in folded DNA and RNA molecules and proliferate close to the melting transition. Using a theory for nucleic acid secondary structures that accounts for the logarithmic entropy c ln m for a loop of length m,
Einert, Thomas R., Netz, Roland R., Orland, Henri   +2 more
core   +1 more source

Furan-PNA : a mildly inducible irreversible interstrand crosslinking system targeting single and double stranded DNA [PDF]

, 2016
We here report on the design and synthesis of tailor-made furan-modified peptide nucleic acid (PNA) probes for covalent targeting of single stranded DNA through a crosslinking strategy.
Corradini, Roberto, Gyssels, Ellen, Madder, Annemieke, Manicardi, Alex   +3 more
core   +2 more sources

Rapid hybridization of nucleic acids using isotachophoresis [PDF]

Proceedings of the National Academy of Sciences, 2012
We use isotachophoresis (ITP) to control and increase the rate of nucleic acid hybridization reactions in free solution. We present a new physical model, validation experiments, and demonstrations of this assay. We studied the coupled physicochemical processes of preconcentration, mixing, and chemical reaction kinetics under ITP.
Moran, Bercovici, Crystal M, Han, Joseph C, Liao, Juan G, Santiago   +3 more
openaire   +2 more sources

Expanding Chemical Space of Nucleic Acid Nanoparticles for Tunable Antiviral‐Like Immunomodulatory Responses and Potent Adjuvant Activity

Advanced Functional Materials, EarlyView.
We introduce a nucleic acid nanoparticle (NANP) platform designed to be rrecognized by the human innate immune system in a regulated manner. By changing chemical composition while maintaining constant architectural parameters, we identify key determinants of immunorecognition enabling the rational design of NANPs with tunable immune activation profiles
Martin Panigaj, Hannah S. Newton, Jian Wang, Laxmi Pandey, Yelixza I. Avilla, Phong Nguyen, Morgan Chandler, Justin Halman, Yasmine Radwan, Stephanie Smith, Elijah Edmondson, Simone Difilippantonio, Chelsea Sanders, Bao Tran, Yongmei Zhao, Shaojun Xie, Edward Cedrone, Barry W. Neun, Meni Wanunu, Nikolay V. Dokholyan, Marina A. Dobrovolskaia, Kirill A. Afonin   +21 more
wiley   +1 more source

CARD In Situ hybridization: Sights and signals [PDF]

, 2018
During the last decade, several strategies have been developed to improve the detection sensitivity ofin situ hybridization (ISH) by amplification of either target nucleic acid sequences prior to ISH (e.g.,in situ PCR), or the detection signals after the
Komminoth, Paul, Speel, Ernst
core  

Development of rapid, automated diagnostics for infectious disease: advances and challenges [PDF]

, 2009
The last 2 years has seen an exponential rise in the amount of research funding made available for the development of rapid diagnostic devices for infectious agents of medical importance. This review reports on several such projects.
Ince, J, McNally, A
core   +1 more source

Electrochemically Driven Dissipative Growth of Affinity Hydrogels for Bioresponsive Interfaces

Advanced Functional Materials, EarlyView.
Voltage pulses drive the growth and reinforcement of hydrogel films under dissipative conditions. This biocompatible strategy enables efficient integration of affinity ligands into the hydrogel matrix, enhancing the selective capture of growth factors and allowing precise temporal control over their release, making them well‐suited as adaptive ...
Roberto Baretta, Marco Frasconi
wiley   +1 more source

Cooperative Oligonucleotides in Purification of Cycle Sequencing Products

BioTechniques, 2000
Nucleic acid hybridization is an essential component in many of today's standard molecular biology techniques. In a recent study, we investigated whether nucleic acid capture could be improved by taking advantage of stacking hybridization, which refers ...
A. Blomstergren, D. O'Meara, M. Lukacs, M. Uhlén, J. Lundeberg   +4 more
doaj   +1 more source

Nucleic acid - protein fingerprints. Novel protein classification based on nucleic acid - protein recognition [PDF]

, 2012
Protein chemistry uses protein description and classification based on molecular mass and isoelectric point as general features. Enzymes are also compared by enzymatic reaction constants, namely Km and kcat values. Proteins are also studied by binding to
Alexander Krylov, Renad Zhdanov
core   +1 more source