Nucleic Acid Thermodynamics Derived from Mechanical Unzipping Experiments [PDF]
Life, 2022 Force-spectroscopy techniques have led to significant progress in studying the physicochemical properties of biomolecules that are not accessible in bulk assays. The application of piconewton forces with laser optical tweezers to single nucleic acids has
Paolo Rissone, Felix Ritort
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MeltR software provides facile determination of nucleic acid thermodynamics [PDF]
Biophysical Reports, 2023 Thermodenaturation (melting) curves of macromolecules are used to determine folding thermodynamic parameters. Notably, this insight into RNA and DNA stability underlies nearest neighbor theory and diverse structure prediction tools.
Jacob P. Sieg +3 more
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Novel isoguanine derivative of unlocked nucleic acid-Investigations of thermodynamics and biological potential of modified thrombin binding aptamer. [PDF]
PLoS ONE, 2018 Thrombin binding aptamer (TBA), is a short DNA 15-mer that forms G-quadruplex structure and possesses anticoagulant properties. Some chemical modifications, including unlocked nucleic acids (UNA), 2'-deoxy-isoguanosine and 2'-deoxy-4-thiouridine were ...
Weronika Kotkowiak +2 more
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Native characterization of nucleic acid motif thermodynamics via non-covalent catalysis [PDF]
Nature Communications, 2016 DNA hybridisation thermodynamics parameters underlie rational design of oligonucleotides for diagnostics and nanotechnology. Here, the authors present an accurate method to measure the free energy of a given DNA structure at specific temperature and ...
Chunyan Wang, Jin H. Bae, David Yu Zhang
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Expanding the Functions of KHSRP Protein: Insights into DNA G‐Quadruplex Binding [PDF]
Advanced ScienceKHSRP (KH‐type splicing regulatory protein) is a multifunctional nucleic acid‐binding protein that regulates various cellular processes, with critical roles in controlling gene expression.
Pasquale Russomanno +9 more
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Optical melting measurements of nucleic acid thermodynamics. [PDF]
Methods Enzymol, 2009 Schroeder SJ, Turner DH.
europepmc +3 more sources
Monovalent metal ion binding promotes the first transesterification reaction in the spliceosome
Nature Communications, 2023 Cleavage and formation of phosphodiester bonds in nucleic acids is accomplished by large cellular machineries composed of both protein and RNA. Long thought to rely on a two-metal-ion mechanism for catalysis, structure comparisons revealed many contain ...
Jana Aupič +4 more
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Thermodynamic framework to assess low abundance DNA mutation detection by hybridization. [PDF]
PLoS ONE, 2017 The knowledge of genomic DNA variations in patient samples has a high and increasing value for human diagnostics in its broadest sense. Although many methods and sensors to detect or quantify these variations are available or under development, the ...
Hanny Willems +7 more
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Native Mass Spectrometry and Nucleic Acid G-Quadruplex Biophysics: Advancing Hand in Hand [PDF]
Accounts of Chemical Research, 2021, 54 (19), pp.3691-3699, 2023 While studying nucleic acids to reveal the weak interactions responsible for their three-dimensional structure and for their interactions with drugs, we also contributed to the field of biomolecular mass spectrometry, both in terms of fundamental understanding and with new methodological developments.
arxiv +1 more source
On binding mechanism exploration of organic amphoteric dye Rhodamine B with natural polymeric DNA
Discover Materials, 2023 Nucleic acids, like DNA and RNA, are molecules that are present in any life form. Their most notable function is to encode biological information.
Senchumbeni Yanthan +1 more
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