Results 1 to 10 of about 2,687,425 (254)

Quantitative thermodynamic predication of interactions between nucleic acid and non-nucleic acid species using Microsoft excel

Computer Methods and Programs in Biomedicine, 2013
Proper design of nucleic acid sequences is crucial for many applications. We have previously established a thermodynamics-based quantitative model to help design aptamer-based nucleic acid probes by predicting equilibrium concentrations of all interacting species.
Jiaqi Zou, Na Li 0043
exaly   +4 more sources

Protein–nucleic acid thermodynamic databases for specific uses

Trends in Biotechnology, 2023
In response to Gromiha and Harini, we review the currently available thermodynamic databases for protein-nucleic acid interactions. These databases are designed for particular uses. We give general comments on them to facilitate browsing and exploration.
Ge-Fei Hao, Guang-Fu Yang
exaly   +4 more sources

Thermodynamics of ligand-nucleic acid interactions.

Methods in enzymology, 1992
Ligand-and protein-DNA equilibria are extremely sensitive to solution conditions (e.g., salt, temperature, and pH), and, in general, the effects of different solution variables are interdependent (i.e., linked). As a result, an assessment of the basis for the stability and specificity of ligand-or protein-DNA interactions requires quantitative studies ...
T M, Lohman, D P, Mascotti
openaire   +2 more sources

Thermodynamics of nucleic acids and their interactions with ligands

Quarterly Reviews of Biophysics, 2000
1. Introduction 2551.1 General thermodynamics 2562. Nucleic acid thermodynamics 2602.1 DNA duplexes 2612.2 RNA duplexes 2632.3 Hybrid DNA–RNA duplexes 2642.4 Hydration 2672.5 Conformational flexibility 2692.6 Thermodynamics 2723. Nucleic acid–ligand interactions 2773.1 Minor groove binders 2783.2 DNA intercalators 2843.3 Triple-helical systems 2883.3.1
A N, Lane, T C, Jenkins
openaire   +2 more sources

Quantitative nucleic acids footprinting: thermodynamic and kinetic approaches

Current Opinion in Biotechnology, 1997
Quantitative footprinting techniques allow a detailed analysis of the thermodynamic forces that characterize nucleic acid-ligand interactions and ligand-induced changes in nucleic acid structure by separately resolving the intrinsic and cooperative Gibbs free energy changes describing the reactions being investigated.
V, Petri, M, Brenowitz
openaire   +2 more sources

A Spectroscopic and Thermodynamic Study of Taxol Nucleic Acid Complexes

Nucleosides and Nucleotides, 1999
The interactions of natural and synthetic polynucleotide double strands with the antitumor agent paclitaxel and the oncological product "Taxol for Injection Concentrate" (abbreviated as taxol) were examined in diluted aqueous solutions by thermal denaturation profiles (Tm), CD spectra and UV-absorption measurements.
G, Bischoff, U, Gromann, S, Lindau, W V, Meister, S, Hoffmann   +4 more
openaire   +2 more sources

Thermodynamic databases for proteins and protein-nucleic acid interactions

Biopolymers, 2001
Thermodynamic data regarding proteins and their interactions are important for understanding the mechanisms of protein folding, protein stability, and molecular recognition. Although there are several structural databases available for proteins and their complexes with other molecules, databases for experimental thermodynamic data on protein stability ...
A, Sarai, M M, Gromiha, J, An, P, Prabakaran, S, Selvaraj, H, Kono, M, Oobatake, H, Uedaira   +7 more
openaire   +2 more sources

Thermodynamic Characterization of Nucleic Acid Nanoparticles Hybridization by UV Melting

2023
The advances in nucleic acid nanotechnology have given rise to various elegantly designed structural complexes fabricated from DNA, RNA, chemically modified RNA strands, and their mixtures. The structural properties of NA nanoparticles (NANP) generally dictate and significantly impact biological function; and thus, it is critical to extract information
Megan, Teter, Ross, Brumett, Abigail, Coffman, Emil F, Khisamutdinov   +3 more
openaire   +2 more sources

Thermodynamic study of hybridization properties of heterochiral nucleic acids

Biochemical and Biophysical Research Communications, 2003
Heterochiral DNA and RNA heptamers, which contained an unnatural L-nucleotide, were synthesized, and thermodynamic analyses of their hybridization properties with complementary DNA and RNA strands were systematically conducted by UV melting experiments.
Hidehito, Urata, Hana, Shimizu, Hidekazu, Hiroaki, Daisuke, Kohda, Masao, Akagi   +4 more
openaire   +2 more sources

Analyzing and predicting the thermodynamic effects of the metabolite trehalose on nucleic acids

Biopolymers, 2010
AbstractThere is a lot of interest in exactly how nucleic acid duplexes are affected by the addition of certain stabilizing and destabilizing metabolites. Unfortunately, the differences in reaction conditions between published reports often precludes a comparison of the results, effectively preventing a cohesive strategy for predicting additive effects
Jonathan L, Hart, Zachary M, Harris, Stephen M, Testa   +2 more
openaire   +2 more sources