Results 1 to 10 of about 10,352 (215)
Nucleic Acid Thermodynamics Derived from Mechanical Unzipping Experiments [PDF]
Life, 2022 Force-spectroscopy techniques have led to significant progress in studying the physicochemical properties of biomolecules that are not accessible in bulk assays. The application of piconewton forces with laser optical tweezers to single nucleic acids has
Paolo Rissone, Felix Ritort
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MeltR software provides facile determination of nucleic acid thermodynamics [PDF]
Biophysical Reports, 2023 Thermodenaturation (melting) curves of macromolecules are used to determine folding thermodynamic parameters. Notably, this insight into RNA and DNA stability underlies nearest neighbor theory and diverse structure prediction tools.
Jacob P. Sieg +3 more
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Optical melting measurements of nucleic acid thermodynamics. [PDF]
Methods Enzymol, 2009 Optical melting experiments provide measurements of thermodynamic parameters for nucleic acids. These thermodynamic parameters are widely used in RNA structure prediction programs and DNA primer design software. This review briefly summarizes the theory and underlying assumptions of the method and provides practical details for instrument calibration ...
Schroeder SJ, Turner DH.
europepmc +4 more sources
Thermodynamics of Nucleic Acid “Shape Readout” by an Aminosugar [PDF]
Biochemistry, 2011 Recognition of nucleic acids is important for our understanding of nucleic acid structure as well as for our understanding of nucleic acid-protein interactions. In addition to the direct readout mechanisms of nucleic acids such as H-bonding, shape recognition of nucleic acids is being increasingly recognized as playing an equally important role in DNA ...
Dev Arya, Nihar Ranjan
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Novel isoguanine derivative of unlocked nucleic acid-Investigations of thermodynamics and biological potential of modified thrombin binding aptamer. [PDF]
PLoS ONE, 2018 Thrombin binding aptamer (TBA), is a short DNA 15-mer that forms G-quadruplex structure and possesses anticoagulant properties. Some chemical modifications, including unlocked nucleic acids (UNA), 2'-deoxy-isoguanosine and 2'-deoxy-4-thiouridine were ...
Weronika Kotkowiak +2 more
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Native characterization of nucleic acid motif thermodynamics via non-covalent catalysis
Nature Communications, 2016 DNA hybridisation thermodynamics parameters underlie rational design of oligonucleotides for diagnostics and nanotechnology. Here, the authors present an accurate method to measure the free energy of a given DNA structure at specific temperature and ...
Chunyan Wang, Jin H. Bae, David Yu Zhang
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Thermodynamic analysis of Zα domain-nucleic acid interactions [PDF]
Biochemical Journal, 2022 Abstract DNA/RNA molecules adopting the left-handed conformation (Z-form) have been attributed with immunogenic properties. However, their biological role and importance has been a topic of debate for many years. The discovery of Z-DNA/RNA binding domains (Zα domains) in varied proteins that are involved in the innate
Bharath Srinivasan +2 more
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Thermodynamic Analysis of Nylon Nucleic Acids [PDF]
ChemBioChem, 2008 AbstractThe stability and structure of nylon nucleic acid duplexes with complementary DNA and RNA strands was examined. Thermal denaturing studies of a series of oligonucleotides that contained nylon nucleic acids (1–5 amide linkages) revealed that the amide linkage significantly enhanced the binding affinity of nylon nucleic acids towards both ...
Yu, Liu +6 more
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NTDB: Thermodynamic Database for Nucleic Acids [PDF]
Nucleic Acids Research, 2001 A new thermodynamic database for normal and modified nucleic acids has been developed. This Thermodynamic Database for Nucleic Acids (NTDB) includes sequence, structure and thermodynamic information as well as experimental methods and conditions. In this release, there are 1851 sequences containing both normal and modified nucleic acids.
Wing Lok Abe Kurtz Chiu +4 more
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Thermodynamic Analysis of Interacting Nucleic Acid Strands [PDF]
SIAM Review, 2007 Motivated by the analysis of natural and engineered DNA and RNA systems, we present the first algorithm for calculating the partition function of an unpseudoknotted complex of multiple interacting nucleic acid strands. This dynamic program is based on a rigorous extension of secondary structure models to the multistranded case, addressing ...
Robert M. Dirks +4 more
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