Results 91 to 100 of about 221,973 (265)

Microfluidic‐Driven Lipid Nanoparticles for Improved miRNA Delivery via Endo‐Lysosomal Trafficking Optimization

Advanced Science, EarlyView.
This study investigates the impact of various post‐processing methods (filtration, sonication, dialysis, and heating) on lipid nanoparticles produced using microfluidics. The nanoparticles are designed for efficient delivery of miRNA into cells. The work highlights how processing conditions influence nanoparticle stability and quality, providing ...
Alicja Kosik‐Kozioł, Michał Pruchniewski, Daniel Rybak, Piotr Jenczyk, Karolina Zakrzewska, Magdalena Bartolewska, Sławomir Błoński, Paweł Nakielski, Filippo Pierini   +8 more
wiley   +1 more source

The protein cost of metabolic fluxes: prediction from enzymatic rate laws and cost minimization

, 2016
Bacterial growth depends crucially on metabolic fluxes, which are limited by the cell's capacity to maintain metabolic enzymes. The necessary enzyme amount per unit flux is a major determinant of metabolic strategies both in evolution and bioengineering.
Bar-Even, Arren, Davidi, Dan, Flamholz, Avi, Liebermeister, Wolfram, Milo, Ron, Noor, Elad   +5 more
core   +3 more sources

A TtAgo‐Driven Autocatalytic Circuit with Thermal‐Enhanced Kinetics for One‐Pot Nucleic Acid Detection

Advanced Science, EarlyView.
This study proposes a universal catalytic DNA circuits termed TACTIC (Thermus thermophilus protein‐driven autocatalytic circuit) for one‐pot detection of DNA and RNA in multiple clinical samples. Integrated with machine learning, TACTIC accurately profile the distinct expression of four extracellular vesicle‐derived miRNAs across different samples and ...
Zuowei Xie, Ruijia Deng, Ben Niu, Yingjie Yang, Shuang Zhao, Hongzhao Yang, Meilin Gong, Jie Luo, Yu Tang, Jing Sheng, Yan Pi, Ming Chen, Kai Chang   +12 more
wiley   +1 more source

Beyond Cocrystals: Hierarchical Functional Assemblies via Noncovalent Synthesis

Advanced Science, EarlyView.
Non‐covalent interactions (NCIs) drive the formation of organic cocrystals with diverse structures and tunable optoelectronic properties. This review explores the essential factors governing these properties, highlighting how the sequential nucleation of cocrystals leads to the self‐assembly of organic hierarchical structures (OHSs).
Ya‐Nan Zhu, Jin Feng, Ying‐Xin Ma, Hong‐Tao Lin, Li‐Wei Xie, Qi‐Liang Peng, Xue‐Dong Wang   +6 more
wiley   +1 more source

Peptide Nucleic Acids Containing Cationic/Amino-Alkyl Modified Bases Promote Enhanced Hybridization Kinetics and Thermodynamics with Single-Strand DNA

, 2023
Frank Podlaski, Stephen Cornwell, Kenny K. Wong, Brian A. McKittrick, Jae‐Hun Kim, Daram Jung, Yeasel Jeon, Kwang‐Bok Jung, Peter Tolias, William T. Windsor   +9 more
openalex   +1 more source

PIK3CA Mutations Downregulate PPT1 to Promote Adipogenesis by Suppressing P300 Depalmitoylation and Phase Separation

Advanced Science, EarlyView.
This study demonstrates that somatic PIK3CA mutations suppress PPT1 expression via activation of the PI3K–AKT–c‐JUN axis. This reduction in PPT1 weakens its interaction with P300, thereby increasing palmitoylation at C1176 of P300 and protecting P300 from lysosomal degradation.
Hongrui Chen, Zening Huang, Rui Chang, Wei Gao, Yajing Qiu, Bin Sun, Chen Hua, Xiaoxi Lin   +7 more
wiley   +1 more source

Programming Next‐Generation Synthetic Biosensors by Genetic Circuit Design

Advanced Science, EarlyView.
Synthetic biology enables genetic circuit‐based biosensing to detect diverse targets, process signals, and transduce them into readable outputs or intracellular regulatory activities. However, field deployment and real‐world application of such synthetic biosensors face considerable challenges in sensitivity, specificity, speed, stability, and ...
Yuanli Gao, Cheng Huang, Jiaxuan Deng, Lei Wang, Baojun Wang   +4 more
wiley   +1 more source

Vacancy defect‐induced electron homing breaks phosphodiester bonds for RNA depletion‐driven cancer therapy

BMEMat, EarlyView.
Illustration of 5% S‐vacancy Bi2S3 mediated phosphodiester bonds cleavage in RNA of hepatocellular carcinoma cells, which suppressing ERI3 expression, inhibiting cell proliferation and promoting apoptosis. Abstract Genome‐wide hypertranscription is a hallmark of malignant progression.
Chuncheng Yang, Zehao Shen, Guangming Xiang, Zhe Liu, Huaxin Liu, Jiabei Li, Ao Wang, Yelin Wu, Peiran Zhao, Xingwu Jiang, Jinghan Wang, Xiaoqing Jiang, Wenbo Bu   +12 more
wiley   +1 more source

The Limitation of the Combination of Transition State Theory and Thermodynamics for the Reactions of Proteins and Nucleic Acids [PDF]

, 2021
Nobuo Shimamoto
openalex   +1 more source

Thermodynamics and Kinetics of Nucleic Acids Folding

, 2023
[spa] En este trabajo doctoral se han investigado las propiedades termodinámicas y cinéticas de los ácidos nucleicos. El principal objetivo era el estudio de los mecanismos de plegamiento que se llevan a cabo en la hibridación de moléculas de ADN y ARN y su caracterización energética. La tesis está organizada en cuatro partes principales. En la Parte I,
openaire   +2 more sources