Results 131 to 140 of about 191,094 (249)

Folding Competition and Dynamic Transformation in DNA Origami: Parallel Versus Antiparallel Crossovers

Small Methods, Volume 9, Issue 6, June 20, 2025.
This dynamic DNA origami features two rotational panels that can be designed into two major configurations using either antiparallel crossovers (AX) or parallel crossovers (PX). Through competitive folding processes, 36 pairs of designs with different key staples are studied, and a bidirectional isothermal transformation is demonstrated, providing ...
Jung Yeon Lee, Qi Yang, Xu Chang, Devanathan Perumal, Fei Zhang   +4 more
wiley   +1 more source

Nonequilibrium hybridization-driven CRISPR/Cas adapter with extended energetic penalty for discrimination of single-nucleotide variants. [PDF]

Nucleic Acids Res
Liu Q, Jiang Z, Li S, Li Y, Wan Y, Hu Z, Ma S, Zou Z, Yang R.   +8 more
europepmc   +1 more source

How We Simulate DNA Origami

Small Methods, Volume 9, Issue 6, June 20, 2025.
This tutorial provides a detailed step‐by‐step description on how to import DNA origami designs into the oxDNA model and characterize the origami's properties (such as equilibrium shape and flexibility) in simulations using free webservers oxView.org and oxDNA.org.
Sarah Haggenmueller, Michael Matthies, Matthew Sample, Petr Šulc   +3 more
wiley   +1 more source

Insights into nucleic acid helix formation from infrared spectroscopy. [PDF]

Biophys J
Schroeder SJ.
europepmc   +1 more source

Dissociation Constant (Kd) Measurement for Small‐Molecule Binding Aptamers: Homogeneous Assay Methods and Critical Evaluations

Small Methods, Volume 9, Issue 6, June 20, 2025.
The characteristics of aptamer is most commonly represented by the dissociation constant (Kd), which is critical for aptamer optimization. Here, the assays used to evaluate Kd, including labeled and label‐free assays, are evaluated and suggestions are made to ensure that true values are obtained, while other methods are cautioned.
Stefen Stangherlin, Yuzhe Ding, Juewen Liu   +2 more
wiley   +1 more source

Force-induced melting and S-DNA pathways for DNA overstretching exhibit distinct kinetics. [PDF]

Nucleic Acids Res
Sundar Rajan V, Levin S, McCauley MJ, Williams MC, Rouzina I, Wilhelmsson LM, Westerlund F.   +6 more
europepmc   +1 more source

Rapid Amplification and Detection of Single‐Stranded Nucleic Acids for Point‐of‐Care Diagnosis

Small Methods, Volume 9, Issue 6, June 20, 2025.
A rapid, point‐of‐care nucleic acid test can greatly benefit healthcare system by providing timely diagnosis for effective treatment and patient management, as well as supporting diseases surveillance for emerging pandemic diseases. Recent advancements in nucleic acids technology have led to rapid assays for single‐stranded nucleic acids that can be ...
Jinglin Fu, Qiaochu Zhang, Shiming Liu, Derek Puyat, Akshay Shah, Alireza Ebrahimimojarad, Sung Won Oh   +6 more
wiley   +1 more source

Optimization of TaqMan-based quantitative PCR diagnosis for Entamoeba histolytica using droplet digital PCR. [PDF]

PLoS Negl Trop Dis
Kawashima A, Yanagawa Y, Chikata T, Shimogawara R, Mizushima D, Tsuchiya K, Yagita K, Gatanaga H, Watanabe K.   +8 more
europepmc   +1 more source

Abstracts

Molecular Oncology, Volume 19, Issue S1, Page 1-895, June 2025.
Abstracts submitted to the ‘EACR 2025 Congress: Innovative Cancer Science’, from 16–19 June 2025 and accepted by the Congress Organising Committee are published in this Supplement of Molecular Oncology, an affiliated journal of the European Association for Cancer Research (EACR).
wiley   +1 more source

Molecular characterization and structural basis of a promiscuous glycosyltransferase for β‐(1,6) oligoglucoside chain glycosides biosynthesis

Plant Biotechnology Journal, Volume 23, Issue 6, Page 2242-2253, June 2025.
We highlight a novel multi‐step O‐glycosyltransferase, UGT94BY1, which exhibits high promiscuity toward triterpene saponins and flavonoid glycosides for the formation of β‐(1,6) oligoglucoside chains. Through crystal structure analysis and theoretical calculations, we uncovered the mechanisms underlying substrate promiscuity and sugar chain extension ...
Zhennan Jiang, Nianhang Chen, Hao‐Tian Wang, Yungang Tian, Xiaoyu Du, Ruibo Wu, Luqi Huang, Zi‐Long Wang, Yuan Yuan   +8 more
wiley   +1 more source