Results 61 to 70 of about 192,096 (245)
DNA Sequential Logic Circuits for Reversible Counters and Dynamic Biomolecular Sensing
In this work, a DNA sequential logic circuit is constructed that combines reusability and autonomy for the first time, including SR (Set‐Reset)‐latches composed of NOR gates, SR‐latches composed of NAND gates, and D (Data)‐latches. After fully utilizing the characteristics of each latch, to counter, toxin and miRNA detection and intracellular dynamic ...
Tianci Xie+7 more
wiley +1 more source
The affinity between a guest and a macrocyclic host depends strongly on the water encapsulated inside the host cavity, with free‐energy variations on the order of 20 kcal mol−1 being possible even in the absence of direct host‐guest interactions. This conclusion–which contrasts alternative views–can be drawn from molecular dynamics simulations for the ...
Jeffry Setiadi+3 more
wiley +2 more sources
Internal Phase Separation in Synthetic DNA Condensates
The modular, programmable system of DNA nanostructures developed provides programmatic control over multiphase condensate behavior, enabling mapping onto a predictive Flory‐Huggins model. This combined experimental and theoretical framework will help address open questions in condensate biophysics and facilitate the rational design of functional ...
Diana A. Tanase+5 more
wiley +1 more source
Decoy DNA Protects Molecular Tension Probes from DNase Degradation
DNA‐based probes are rapidly degraded by DNases in live‐cell environments, limiting their use. We introduce a decoy DNA strategy, where an excess of unmodified double‐stranded DNA acts as a competitive substrate for DNases, protecting functional DNA probes and significantly extending their stability for long‐term live‐cell studies.
Hongyuan Zhang+2 more
wiley +2 more sources
The Limitation of the Combination of Transition State Theory and Thermodynamics for the Reactions of Proteins and Nucleic Acids [PDF]
Nobuo Shimamoto
openalex +1 more source
Design of nucleic acid sequences for DNA computing based on a thermodynamic approach
We have developed an algorithm for designing multiple sequences of nucleic acids that have a uniform melting temperature between the sequence and its complement and that do not hybridize non-specifically with each other based on the minimum free energy (DeltaG (min)).
Tanaka, Fumiaki+3 more
openaire +4 more sources
The aberrant adenosine metabolism leads to chemoresistance and immunosuppressive microenvironment. A versatile albumin‐polyplexes conjugated nanoparticle B‐PDEA@CPA is prepare for the codelivery of gemcitabine and siCD39. The nanoparticle releases gemcitabine‐loaded albumin upon microenvironment to achieve promoted penetration and cellular uptake ...
Hongrui Fan+10 more
wiley +1 more source
Thiol reductants activate dichalcogenide probes by a cascade of partly reversible steps. Careful dichalcogenide engineering can tune the rates of these steps separately, to aim at probes that are kinetically selective for specific oxidoreductases in live cells.
Lukas Zeisel+6 more
wiley +2 more sources
It is synthesized precision‐arranged DNA origami plasmonic nanoantennas for multiplexed and intelligent warning of bone loss induced by microgravity and radiation. The ordered nanoantennas simultaneously and accurately detected calcium ions, interleukin‐6, and microRNA‐214 in serum from mice exposed to microgravity and radiation, with performance ...
Yufan Ling+14 more
wiley +1 more source
When cellular iron is low, bacterioferritin (Bfr) releases its stored iron through a reductive process dependent on the [2Fe–2S] ferredoxin Bfd. Why accessing stored iron should depend on an iron‐requiring protein when iron is scarce is unclear. Here, we show that the Bfd [2Fe–2S] cluster is sensitive to oxidative stress, suggesting that it may act as ...
Justin M. Bradley+4 more
wiley +2 more sources