Results 91 to 100 of about 6,062,195 (387)

Effect of preparation procedures on intensity of radioautographic labeling is studied [PDF]

, 1967
Effects of tissue preparation and extractive procedures on the intensity of radioautographic labeling are presented in terms of mean grain count per cell in cells labeled with tritiated precursors of proteins or nucleic acids.
Baserga, R., Kisieleski, W. E.
core   +1 more source

TFE and Spt4/5 open and close the RNA polymerase clamp during the transcription cycle [PDF]

, 2016
Transcription is an intrinsically dynamic process and requires the coordinated interplay of RNA polymerases (RNAPs) with nucleic acids and transcription factors.
Gietl, Andreas, Grohmann, Dina, Schulz, Sarah, Smollett, Katherine, Tinnefeld, Philip, Werner, Finn   +5 more
core   +1 more source

Incorporation of bridged nucleic acids into CRISPR RNAs improves Cas9 endonuclease specificity

Nature Communications, 2018
Off-target DNA cleavage is a paramount concern when applying CRISPR-Cas9 gene-editing technology to functional genetics and human therapeutic applications.
Christopher R. Cromwell, Keewon Sung, Jinho Park, Amanda R. Krysler, J. Jovel, Seong Keun Kim, Basil P. Hubbard   +6 more
semanticscholar   +1 more source

Highly multiplexed digital PCR assay for simultaneous quantification of variant allele frequencies and copy number alterations of KRAS and GNAS in pancreatic cancer precursors

Molecular Oncology, EarlyView.
Combining melting curve analysis enhances the multiplexing capability of digital PCR. Here, we developed a 14‐plex assay to simultaneously measure single nucleotide mutations and amplifications of KRAS and GNAS, which are common driver genes in pancreatic cancer precursors. This assay accurately quantified variant allele frequencies in clinical samples
Junko Tanaka, Tatsuo Nakagawa, Yusuke Ono, Yoshio Kamura, Takeshi Ishida, Hidemasa Kawabata, Kenji Takahashi, Hiroki Sato, Andrew S. Liss, Yusuke Mizukami, Takahide Yokoi   +10 more
wiley   +1 more source

Determinants of Genomic RNA Encapsidation in the Saccharomyces cerevisiae Long Terminal Repeat Retrotransposons Ty1 and Ty3

Viruses, 2016
Long-terminal repeat (LTR) retrotransposons are transposable genetic elements that replicate intracellularly, and can be considered progenitors of retroviruses.
Katarzyna Pachulska-Wieczorek, Stuart F.J. Le Grice, Katarzyna J. Purzycka   +2 more
doaj   +1 more source

UVB radiation induced effects on cells studied by FTIR spectroscopy

, 2010
We have made a preliminary analysis of the results about the eVects on tumoral cell line (lymphoid T cell line Jurkat) induced by UVB radiation (dose of 310 mJ/cm^2) with and without a vegetable mixture. In the present study, we have used two techniques:
A. Congiu Castellano, B Fadeel, C Di Pietro, D Pozzi, D. Pozzi, H Tuschl, I. Silvestri, JL Ravant, Lucia Di Giambattista, M Engeland Van, M Hagenhofer, M-E Legaz, M. Grandi, N Gault, N Jamin, P. Grimaldi, PG Andrus, S Gaudenzi, S. Gaudenzi, S. Morrone, SY Chor, Z Novak   +21 more
core   +1 more source

Multi‐omic characterization of consensus molecular subtype 1 (CMS1) colorectal cancer with dampened immune response improves precision medicine

Molecular Oncology, EarlyView.
This study highlights the importance of multi‐omic analyses in characterizing colorectal cancers. Indeed, our analysis revealed a rare CMS1 exhibiting dampened immune activation, including reduced PD‐1 expression, moderate CD8+ T‐cell infiltration, and suppressed JAK/STAT pathway.
Livia Concetti, Manuel Scimeca, Julia Bischof, Jonathan Woodsmith, Massimiliano Agostini, Cristina Fiorani, Yufang Shi, Eleonora Candi, Gerry Melino, Alessandro Mauriello, Giuseppe S. Sica   +10 more
wiley   +1 more source

Phosphate steering by Flap Endonuclease 1 promotes 5′-flap specificity and incision to prevent genome instability

Nature Communications, 2017
Flap Endonuclease 1 is a DNA replication and repair enzyme indispensable for maintaining genomic stability. Here the authors provide mechanistic details on how FEN1 selects for 5′-flaps and promotes catalysis to avoid large-scale repeat expansion by a ...
Susan E. Tsutakawa, Mark J. Thompson, Andrew S. Arvai, Alexander J. Neil, Steven J. Shaw, Sana I. Algasaier, Jane C. Kim, L. David Finger, Emma Jardine, Victoria J.B. Gotham, Altaf H. Sarker, Mai Z. Her, Fahad Rashid, Samir M. Hamdan, Sergei M. Mirkin, Jane A. Grasby, John A. Tainer   +16 more
doaj   +1 more source

MRNIP/C5orf45 Interacts with the MRN Complex and Contributes to the DNA Damage Response

Cell Reports, 2016
Through an RNAi-based screen for previously uncharacterized regulators of genome stability, we have identified the human protein C5orf45 as an important factor in preventing the accumulation of DNA damage in human cells.
Christopher J. Staples, Giancarlo Barone, Katie N. Myers, Anil Ganesh, Ian Gibbs-Seymour, Abhijit A. Patil, Ryan D. Beveridge, Caroline Daye, Richard Beniston, Sarah Maslen, Ivan Ahel, J. Mark Skehel, Spencer J. Collis   +12 more
doaj   +1 more source

Hydrophobicity and Unique Folding of Selected Polymers

, 1998
In suitable environments, proteins, nucleic acids and certain synthetic polymers fold into unique conformations. This work shows that it is possible to construct lattice models of foldable heteropolymers by expressing the energy only in terms of ...
Vendruscolo, Michele
core   +1 more source