Results 61 to 70 of about 6,062,195 (387)

Fluorescence-based quantification of messenger RNA and plasmid DNA decay kinetics in extracellular biological fluids and cell extracts [PDF]

, 2020
Extracellular and intracellular degradation of nucleic acids remains an issue in non-viral gene therapy. Understanding biodegradation is critical for the rational design of gene therapeutics in order to maintain stability and functionality at the target ...
Braeckmans, Kevin, De Smedt, Stefaan, Raes, Laurens, Remaut, Katrien, Rombouts, Koen, Xiong, Ranhua, Zhang, Heyang   +6 more
core   +1 more source

Evolutionary interplay between viruses and R‐loops

FEBS Letters, EarlyView.
Viruses interact with specialized nucleic acid structures called R‐loops to influence host transcription, epigenetic states, latency, and immune evasion. This Perspective examines the roles of R‐loops in viral replication, integration, and silencing, and how viruses co‐opt or avoid these structures.
Zsolt Karányi, Zita Képes, Zoltán Szabó, Eszter Csoma, Lóránt Székvölgyi   +4 more
wiley   +1 more source

Na+ selective structural switch from an intramolecular triplex to tetrad stabilised by non-canonical mispairs in double repeat of Arabidopsis thaliana telomere (T3AG3)2

Biochemistry and Biophysics Reports
DNA is polymorphic, as with four nucleobases, it can be configured in a number of secondary structures. The four-stranded DNA structures consisting of G-tetrads have especially been intriguing because of their proven existence in human cells.
Aparna Bansal, Priyanka Phogat, Shrikant Kukreti   +2 more
doaj   +1 more source

Purification tags markedly affect self‐aggregation of CPEB3

FEBS Letters, EarlyView.
Although recombinant proteins are used to study protein aggregation in vitro, uncleaved tags can interfere with accurate interpretation. Our findings demonstrate that His₆‐GFP and His₁₂ tags significantly affect liquid droplet and amyloid fibril formation in the intrinsically disordered region (IDR) of mouse cytoplasmic polyadenylation element‐binding ...
Harunobu Saito, Yujin Lee, Motoharu Ueno, Naotaka Sekiyama, Masatomo So, Ayako Furukawa, Kenji Sugase   +6 more
wiley   +1 more source

Towards improved anticancer properties of the G-quadruplex-forming T40231 aptamer through increased flexibility of nucleoside residues

Scientific Reports
In this article, the T40231 aptamer, an anticancer agent, originally known for its ability to inhibit STAT3, was altered with UNAs to enhance its flexibility in the loops or G-tetrads.
Weronika Kotkowiak, Natalia Bartyś, Anna Pasternak   +2 more
doaj   +1 more source

Endogenous Ribonucleases: Therapeutic Targeting of the Transcriptome Through Oligonucleotide-Triggered RNA Inactivation

Biomolecules
The selective regulation of gene expression at the RNA level represents a rapidly evolving field offering substantial clinical potential. This review examines the molecular mechanisms of intracellular enzymatic systems that utilize single-stranded ...
Daria A. Chiglintseva, Olga A. Patutina, Marina A. Zenkova   +2 more
doaj   +1 more source

Irisin Is Related to Non-Alcoholic Fatty Liver Disease (NAFLD)

Biomedicines, 2022
Irisin is a cytokine involved in many metabolic pathways occurring, among others, in muscles, adipose tissue and liver. Thus, fluctuations in irisin levels are suggested to be related to metabolic diseases.
Marcin Kosmalski, Józef Drzewoski, Izabela Szymczak-Pajor, Andrzej Zieleniak, Melania Mikołajczyk-Solińska, Jacek Kasznicki, Agnieszka Śliwińska   +6 more
doaj   +1 more source

Quest for space: Tenacity of DNA, Protein, and Lipid macromolecules in intracellular crowded environment

Biomolecular Concepts
The biochemical processes in the cellular milieu involving biomacromolecular interaction usually occur in crowded and heterogeneous environments, impacting their structure, stability, and reactivity.
Phogat Priyanka, Bansal Aparna, Nain Nishu, Khan Shoaib, Saso Luciano, Kukreti Shrikant   +5 more
doaj   +1 more source

Exosome-delivered microRNAs promote IFN-α secretion by human plasmacytoid DCs via TLR7 [PDF]

, 2018
The excessive production of type I IFNs is a hallmark and a main pathogenic mechanism of many autoimmune diseases, including systemic lupus erythematosus (SLE).
Andreoli, Laura, Bergese, Paolo, Bosisio, Daniela, Busatto, Sara, D'Oro, Ugo, Giannello, Veronica, Salvi, Valentina, Sozzani, Silvano, Tincani, Angela, Zingoni, Alessandra   +9 more
core   +1 more source

Detection of Microbial Infections Through Innate Immune Sensing of Nucleic Acids.

Annual Review of Microbiology, 2018
Microbial infections are recognized by the innate immune system through germline-encoded pattern recognition receptors (PRRs). As most microbial pathogens contain DNA and/or RNA during their life cycle, nucleic acid sensing has evolved as an essential ...
Xiaojun Tan, Lijun Sun, Jueqi Chen, Zhijian J. Chen   +3 more
semanticscholar   +1 more source