Results 1 to 10 of about 1,595 (133)

Nucleoside deaminases: the key players in base editing toolkit [PDF]

Biophysics Reports, 2023
The development of nucleoside deaminase-containing base editors realized targeted single base change with high efficiency and precision. Such nucleoside deaminases include adenosine and cytidine deaminases, which can catalyze adenosine-to-inosine (A-to-I) and cytidine-to-uridine (C-to-U) conversion respectively.
Bei Yang, Jiangchao Xiang
exaly   +4 more sources

Synergistic HMGN1 and VP64 Fusions Potentiate High-Precision and PAM-Flexible Base Editing. [PDF]

Adv Sci (Weinh)
A novel CDA1Δ‐SpRY architecture fused with HMGN1 and VP64 yields a nearly PAM‐less base editing platform. By focusing cytosine conversion predominantly at position −18, this synergistic complex ensures highly precise targeting. Demonstrating enhanced efficiency across diverse models, including yeast and rice, the platform offers a robust solution for ...
Luo X, Qu Y, Ye Z, Li Z, Zhang Y, Luo L, Li S, Zhao W, Wang M, Bock R, Wan J, Tan J.   +11 more
europepmc   +2 more sources

Contribution of Cytidine Deaminase to Thymidylate Biosynthesis in Trypanosoma brucei: Intracellular Localization and Properties of the Enzyme

mSphere, 2019
Cytidine deaminase (CDA) is a pyrimidine salvage enzyme that catalyzes cytidine and deoxycytidine hydrolytic deamination to yield uridine and deoxyuridine.
Ana Moro-Bulnes, Víctor M. Castillo-Acosta, Maria Valente, Juana Carrero-Lérida, Guiomar Pérez-Moreno, Luis Miguel Ruiz-Pérez, Dolores González-Pacanowska   +6 more
doaj   +1 more source

An interferon-like small chemical compound CDM-3008 suppresses hepatitis B virus through induction of interferon-stimulated genes.

PLoS ONE, 2019
Oral administration of nucleotide analogues and injection of interferon-α (IFNα) are used to achieve immediate suppression in replication of hepatitis B virus (HBV).
Yutaka Furutani, Mariko Toguchi, Yumi Shiozaki-Sato, Xian-Yang Qin, Etsuko Ebisui, Shoko Higuchi, Masayuki Sudoh, Harukazu Suzuki, Nobuaki Takahashi, Koichi Watashi, Takaji Wakita, Hideaki Kakeya, Soichi Kojima   +12 more
doaj   +1 more source

Nucleoside Analogs in ADAR Guide Strands Enable Editing at 5′-GA Sites

Biomolecules
Adenosine Deaminases Acting on RNA (ADARs) are members of a family of RNA editing enzymes that catalyze the conversion of adenosine into inosine in double-stranded RNA (dsRNA).
Aashrita Manjunath, Jeff Cheng, Kristen B Campbell, Casey S. Jacobsen, Herra G. Mendoza, Leila Bierbaum, Victorio Jauregui-Matos, Erin E. Doherty, Andrew J. Fisher, Peter A. Beal   +9 more
doaj   +1 more source

Biosynthesis and Genetic Tuning of Guanine Crystals via Metabolic Engineering of Yeast

Angewandte Chemie, Volume 138, Issue 40, 28 September 2026.
We demonstrate how yeast cells can be harnessed as cellular factories for the synthesis of optically functional guanine crystals, where genetic editing is used to tune crystal size, order and optical properties. This demonstrates a powerful new biosynthetic approach for the development of sustainable and biocompatible optical pigments, which utilizes ...
Jerome N. Janssen, Yahel Fishman, Yi Li, Avital Wagner, Mariela J. Pavan, Zohar Paleiov, Tali Lemcoff, Rotem Lichtenstein‐Wolfheim, Alexander Upcher, Einat Nativ‐Roth, Amir Aharoni, Benjamin A. Palmer   +11 more
wiley   +2 more sources

Synthesis of 1,4-azaphosphinine nucleosides and evaluation as inhibitors of human cytidine deaminase and APOBEC3A

Beilstein Journal of Organic Chemistry
Nucleoside and polynucleotide cytidine deaminases (CDAs), such as CDA and APOBEC3, share a similar mechanism of cytosine to uracil conversion. In 1984, phosphapyrimidine riboside was characterised as the most potent inhibitor of human CDA, but the quick ...
Maksim V. Kvach, Stefan Harjes, Harikrishnan M. Kurup, Geoffrey B. Jameson, Elena Harjes, Vyacheslav V. Filichev   +5 more
doaj   +1 more source

A C‐Nucleoside Analogue of Cordycepin With High Metabolic Stability and Potent Anti‐Psoriatic Activity via Microneedle Delivery

Advanced Science, EarlyView.
This work identified CPD3a as a potent, stable C‐nucleoside cordycepin derivative. When formulated into microneedle array for topical treatment, it ameliorated psoriasis by rebalancing immunity and enhancing antioxidant defenses. ABSTRACT Psoriasis is a chronic inflammatory disorder characterized by immune dysregulation and epidermal hyperplasia ...
Wenfang Pan, Xinyue Shao, Yuanchen Zhong, Xujie Sun, Lixuan Yin, Zongyan He, Tianqun Lang, Yuanchao Xie   +7 more
wiley   +1 more source

The Cancer Cell Metabolic Reprogramming Remodels the Tumor Microenvironment: Molecular Mechanisms and Therapeutic Strategies

Advanced Science, EarlyView.
This review elucidates how cancer cell metabolic reprogramming—across glucose, lipid, amino acid, and nucleotide pathways—remodels the tumor microenvironment to suppress anti‐tumor immunity and promote immune escape. Targeting these metabolic axes offers promising strategies to overcome immunotherapy resistance and enhance cancer treatment.
Guoqing Xiang, Zhicheng Zhou, Xue Jiang, Zhenguang Du, Yangyang Zhan, Xinli Liu   +5 more
wiley   +1 more source

Ribonucleotides Amplify Mitochondrial DNA‐Driven cGAS–STING Activation via FdUMP‐ribonucleotide Hybrid Particles to Potentiate Chemoimmunotherapy

Advanced Science, EarlyView.
This work develops a metabolic sharing strategy that employs cytidine monophosphate (CMP) to competitively reprogram floxuridine (FUDR) pyrimidine metabolism in colorectal tumor cells. Elevated intracellular FdUMP induces nucleotide imbalance, drives mitochondrial DNA release and cGAS‐STING innate immune cascade activation, and potentiates robust ...
Chao Wang, Chenxuan Zhao, Yesong Yao, Zhiwen Zou, Anxin Shi, Zhuo Feng, Mingkang Yang, Yue Yu, Yang Liu, Xuehui Rui, Jinhui Wu   +10 more
wiley   +1 more source