Results 61 to 70 of about 115,586 (202)

An enzymatic flow-based preparative route to vidarabine [PDF]

, 2020
The bi-enzymatic synthesis of the antiviral drug vidarabine (arabinosyladenine, ara-A), catalyzed by uridine phosphorylase from Clostridium perfringens (CpUP) and a purine nucleoside phosphorylase fromAeromonas hydrophila (AhPNP), was re-designed under ...
A. Pinto, C. Previtali, D. Ubiali, E. Calleri, F. Annunziata, F. Rinaldi, G. Speranza, L. Tamborini, M. Terreni, P. Conti, T. Bavaro   +10 more
core   +1 more source

Effect of Renal Impairment on the Pharmacokinetics and Safety of Doxecitine and Doxribtimine: A Single‐Dose Phase 1 Study

Clinical Pharmacology in Drug Development, Volume 15, Issue 2, February 2026.
Abstract This Phase 1 study investigated the pharmacokinetics and safety of a single dose of the FDA‐approved doxecitine and doxribtimine (266.6 mg/kg; 133.3 mg/kg of deoxycytidine [dC] and deoxythymidine [dT]) in participants with severe (n = 8) or moderate (n = 8) renal impairment (estimated glomerular filtration rate [eGFR] 15–29 mL/min/1.73 m2 and ...
Aravind Mittur, Susan A. VanMeter
wiley   +1 more source

Efficient whole-cell biocatalytic synthesis of 2′-deoxy-2′-fluoroadenosine, a key building block for nucleic acid drugs

Synthetic and Systems Biotechnology
2′-Deoxy-2′-fluoroadenosine (2′-F-dA) is a nucleoside analogue used as a key building block for oligonucleotide drugs. It can be biosynthesized from a low-cost 2′-deoxy-2′-fluorouridine via one-pot transglycosylation catalyzed by a thymidine ...
Dandan Feng, Tianshu Zhang, Guan Zhou, Yanli Cao, Quan Luo, Huifang Xu, Xuefeng Lu   +6 more
doaj   +1 more source

Biocatalytic Construction of a Chemical Exchange Saturation Transfer Magnetic Resonance Imaging Nucleoside Probe: Synthesis and Evaluation of 5‐Methyl‐5,6‐Dihydrothymidine

Advanced Synthesis &Catalysis, Volume 368, Issue 1, January 2026.
Al‐Hilfi et al. present a biocatalytic strategy for synthesizing 5‐methyl‐5,6‐dihydrothymidine (5‐MDHT), a sensitive MRI contrast agent. The study demonstrates that recombinant enzyme catalysis offers an efficient, sustainable, and eco‐friendly alternative to traditional chemical synthesis for producing clinically relevant imaging probes.
Aimen Al‐Hilfi, E. Alejandro Castellanos Franco, Connor J. Grady, Zinia Mohanta, Michael T. McMahon, Milana Bazayeva, Zhen Li, Kenneth M. Merz Jr., Assaf A. Gilad   +8 more
wiley   +1 more source

A Rapid Colorimetric Method for Determining Calcium, Inorganic Phosphorus, and Zinc in Human Milk Using Clinical Laboratory Reagents

Journal of Clinical Laboratory Analysis, Volume 40, Issue 2, January 2026.
We evaluated a colorimetric assay using clinical reagents for determining Ca, IP, and Zn in human milk. Significant correlations were observed between Ca, IP, and Zn levels determined by colorimetric assay and Ca, total P, and Zn levels determined by ICP‐AES.
Miori Tanaka, Misaki Mochida, Midori Date, Katsumi Mizuno   +3 more
wiley   +1 more source

Phosphomimetic mutations near active sites of proteins in Thermus thermophilus suggest a widespread regulatory mechanism

FEBS Open Bio, Volume 15, Issue 12, Page 1987-2000, December 2025.
In Thermus thermophilus, more than half of the phosphorylation sites identified by proteomic analysis are located near the active site. All phosphomimetic mutants of phosphosites of six enzymes belonging to different families showed severely reduced activity compared with the wild‐type, particularly in the turnover number.
Anzu Nishiwaki, Hiroki Okanishi, Yoshikatsu Kanai, Ryoji Masui   +3 more
wiley   +1 more source

Still a Long Way to Fully Understanding the Molecular Mechanism of Escherichia coli Purine Nucleoside Phosphorylase [PDF]

, 2013
The results of several decades of studying the catalytic mechanism of Escherichia colt purine nucleoside phosphorylases (PNP) by solution studies and crystal structure determinations are presented.
Bzowska, Agnieszka, Luić, Marija, Mikleušević, Goran, Narczyk, Marta, Wielgus-Kutrowska, Beata, Štefanić, Zoran   +5 more
core   +2 more sources

Characterization and Antibody Preparation of the Gene Products of Grouper Iridovirus ORF5L

Aquaculture, Fish and Fisheries, Volume 5, Issue 6, December 2025.
ABSTRACT Grouper iridovirus (GIV) is a large DNA virus belonging to the family Iridoviridae and genus Ranavirus. It consists of 139,793 base pairs (bp) and 120 open reading frames (ORFs). GIV is a key pathogen of farmed fish worldwide. The functional roles of viral genes must be determined for the early diagnosis, treatment and prevention of viral ...
Chu‐Fan Cheng, Hsiang‐Chieh Chuang, Yu‐Shen Lai   +2 more
wiley   +1 more source

Properties of purine nucleoside phosphorylases from spores and vegetative cells of Bacillus cereus and their modification by orthophosphate.

Journal of Biological Chemistry, 1969
The purine nucleoside phosphorylases (PN-phosphorylase) from spores and vegetative cells of Bacillus cereus were each purified to electrophoretic homogeneity.
H. Engelbrecht, H. Sadoff
semanticscholar   +1 more source

Identification of Candidate Blood Biomarkers of Recombinant Human Erythropoietin Administration Using Targeted Polar Metabolomics by HILIC‐MS/MS

Drug Testing and Analysis, Volume 17, Issue 12, Page 2323-2332, December 2025.
Using targeted HILIC‐MS/MS metabolomics, inosine and hypoxanthine were identified as potential biomarkers of EPO administration. Their consistent and sensitive plasma responses highlight their promise for inclusion in the Athlete Biological Passport (ABP), warranting further investigation to enhance anti‐doping detection strategies. ABSTRACT Increasing
Olivier Salamin, Lejla Ramic, Raul Nicoli, Serge Rudaz, Davy Guillarme, Tiia Kuuranne   +5 more
wiley   +1 more source