A cytidine deaminase-like protein modulates pyrimidine nucleotide homeostasis in Trypanosoma brucei. [PDF]
Sci RepAbstract Pyrimidine nucleotide homeostasis is critical for DNA replication and cell viability, yet its regulation in Trypanosoma brucei, the causative agent of African trypanosomiasis, remains poorly understood. Here, we characterize a T. brucei cytidine deaminase-like protein (TbCDA-like), a kinetoplastid-specific enzyme absent in mammals ...
Moro-Bulnes A +7 more
europepmc +6 more sources
The preferred nucleotide contexts of the AID/APOBEC cytidine deaminases have differential effects when mutating retrotransposon and virus sequences compared to host genes. [PDF]
PLoS Computational Biology, 2017 The AID / APOBEC genes are a family of cytidine deaminases that have evolved in vertebrates, and particularly mammals, to mutate RNA and DNA at distinct preferred nucleotide contexts (or "hotspots") on foreign genomes such as viruses and retrotransposons.
Jeffrey Chen, Thomas MacCarthy
doaj +3 more sources
A Plug-in system for reprogramming the editing patterns of base editors [PDF]
Nature CommunicationsDNA base editors are transformative genome editing tools that enable nucleotide conversions without inducing double-stranded DNA breaks, making them promising for correcting genetic mutations.
Chaoyue Zhong +9 more
doaj +2 more sources
Distinct Phage‐Encoded Enzymes for Substitution of Deoxythymidine by Deoxyuridine in Phage Genomes [PDF]
Advanced ScienceDNA base modification is a common strategy used by bacteriophages to evade host immune detection. A prominent example is dU‐DNA, where thymidine is globally replaced with 2′‐deoxyuridine.
Yating Li +7 more
doaj +2 more sources
Efficient, scalable, and near-nucleotide-resolution profiling of protein occupancy in the genome with deaminases. [PDF]
Proc Natl Acad Sci U S AChang L, Ren B.
europepmc +4 more sources
The RNA Binding Specificity of Human APOBEC3 Proteins Resembles That of HIV-1 Nucleocapsid. [PDF]
PLoS Pathogens, 2016 The APOBEC3 (A3) cytidine deaminases are antiretroviral proteins, whose targets include human immunodeficiency virus type-1 (HIV-1). Their incorporation into viral particles is critical for antiviral activity and is driven by interactions with the RNA ...
Ashley York +3 more
doaj +5 more sources
Replication protein A (RPA) hampers the processive action of APOBEC3G cytosine deaminase on single-stranded DNA. [PDF]
PLoS ONE, 2011 Editing deaminases have a pivotal role in cellular physiology. A notable member of this superfamily, APOBEC3G (A3G), restricts retroviruses, and Activation Induced Deaminase (AID) generates antibody diversity by localized deamination of cytosines in DNA.
Artem G Lada +6 more
doaj +1 more source
Molecular Therapy: Nucleic Acids, 2021 Base editors are RNA-guided deaminases that enable site-specific nucleotide transitions. The targeting scope of these Cas-deaminase fusion proteins critically depends on the availability of a protospacer adjacent motif (PAM) at the target locus and is ...
Lukas Villiger +5 more
doaj +1 more source
A split and inducible adenine base editor for precise in vivo base editing
Nature Communications, 2023 DNA base editors use deaminases fused to a programmable DNA-binding protein for targeted nucleotide conversion. However, the most widely used TadA deaminases lack post-translational control in living cells.
Hongzhi Zeng +9 more
doaj +1 more source
Disruption of Transcriptional Coactivator Sub1 Leads to Genome-Wide Re-distribution of Clustered Mutations Induced by APOBEC in Active Yeast Genes. [PDF]
PLoS Genetics, 2015 Mutations in genomes of species are frequently distributed non-randomly, resulting in mutation clusters, including recently discovered kataegis in tumors. DNA editing deaminases play the prominent role in the etiology of these mutations.
Artem G Lada +6 more
doaj +1 more source