A cytidine deaminase-like protein modulates pyrimidine nucleotide homeostasis in Trypanosoma brucei. [PDF]
Sci RepAbstract Pyrimidine nucleotide homeostasis is critical for DNA replication and cell viability, yet its regulation in Trypanosoma brucei, the causative agent of African trypanosomiasis, remains poorly understood. Here, we characterize a T. brucei cytidine deaminase-like protein (TbCDA-like), a kinetoplastid-specific enzyme absent in mammals ...
Moro-Bulnes A +7 more
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The preferred nucleotide contexts of the AID/APOBEC cytidine deaminases have differential effects when mutating retrotransposon and virus sequences compared to host genes. [PDF]
PLoS Computational Biology, 2017 The AID / APOBEC genes are a family of cytidine deaminases that have evolved in vertebrates, and particularly mammals, to mutate RNA and DNA at distinct preferred nucleotide contexts (or "hotspots") on foreign genomes such as viruses and retrotransposons.
Jeffrey Chen, Thomas MacCarthy
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Efficient, scalable, and near-nucleotide-resolution profiling of protein occupancy in the genome with deaminases. [PDF]
Proc Natl Acad Sci U S AChang L, Ren B.
europepmc +4 more sources
Nucleotide sequence of the gene for threonine deaminase (ILV1) of Saccharomyces cerevisiae [PDF]
Carlsberg Research Communications, 1984 The 1,728 base pair coding region of theILV1 gene and the adjacent 777 base pair upstream and 261 base pair downstream regions were sequenced. The size of the polypeptide deduced from the coding region is larger than previously determined values for the molecular weight of threonine deaminase, suggesting that a peptide with a molecular weight of about ...
Morten C. Kielland‐Brandt +3 more
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Surface AMP deaminase 2 as a novel regulator modifying extracellular adenine nucleotide metabolism [PDF]
The FASEB Journal, 2021 Abstract Adenine nucleotides represent crucial immunomodulators in the extracellular environment. The ectonucleotidases CD39 and CD73 are responsible for the sequential catabolism of ATP to adenosine via AMP, thus promoting an anti‐inflammatory milieu induced by the “adenosine halo”.
Lisa Ehlers +8 more
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Distinct Phage‐Encoded Enzymes for Substitution of Deoxythymidine by Deoxyuridine in Phage Genomes [PDF]
Advanced ScienceDNA base modification is a common strategy used by bacteriophages to evade host immune detection. A prominent example is dU‐DNA, where thymidine is globally replaced with 2′‐deoxyuridine.
Yating Li +7 more
doaj +2 more sources
Single nucleotide editing without DNA cleavage using CRISPR/Cas9‐deaminase in the sea urchin embryo [PDF]
Developmental Dynamics, 2017 Background: A single base pair mutation in the genome can result in many congenital disorders in humans. The recent gene editing approach using CRISPR/Cas9 has rapidly become a powerful tool to replicate or repair such mutations in the genome. These approaches rely on cleaving DNA, while presenting unexpected risks.
Saba Shevidi +4 more
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Adenine Nucleotide Pool Perturbation Is a Metabolic Trigger for AMP Deaminase Inhibitor-Based Herbicide Toxicity [PDF]
Plant Physiology, 2007 AMP deaminase (AMPD) is essential for plant life, but the underlying mechanisms responsible for lethality caused by genetic and herbicide-based limitations in catalytic activity are unknown. Deaminoformycin (DF) is a synthetic modified nucleoside that is taken up by plant cells and 5'-phosphorylated into a potent transition state-type inhibitor of AMPD.
Richard L. Sabina +4 more
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Nucleotide Weight Matrices Reveal Ubiquitous Mutational Footprints of AID/APOBEC Deaminases in Human Cancer Genomes [PDF]
Cancers, 2019 Cancer genomes accumulate nucleotide sequence variations that number in the tens of thousands per genome. A prominent fraction of these mutations is thought to arise as a consequence of the off-target activity of DNA/RNA editing cytosine deaminases.
Igor B. Rogozin +8 more
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Proteus mirabilis amino acid deaminase: cloning, nucleotide sequence, and characterization of aad [PDF]
Journal of Bacteriology, 1995 Proteus, Providencia, and Morganella species produce deaminases that generate alpha-keto acids from amino acids. The alpha-keto acid products are detected by the formation of colored iron complexes, raising the possibility that the enzyme functions to secure iron for these species, which do not produce traditional siderophores. A gene encoding an amino
G Massad, Hui Zhao, Harry L. T. Mobley
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