Results 261 to 270 of about 280,333 (306)

Single-Nucleotide Polymorphism Mapping

2006
Single-nucleotide polymorphism (SNP) mapping is the easiest and most reliable way to map genes in Caenorhabditis elegans. SNPs are extremely dense and usually have no associated phenotype, making them ideal markers for mapping. SNP mapping has three steps.
M Wayne Davis, Marc Hammarlund, Davis M Wayne   +2 more
exaly   +3 more sources

Probing RNA Structure and Function by Nucleotide Analog Interference Mapping [PDF]

Current Protocols in Nucleic Acid Chemistry, 2004
Nucleotide analog interference mapping (NAIM) can be used to simultaneously, yet individually, identify structurally or catalytically important functional groups within an RNA molecule.
Scott A Strobel
exaly   +2 more sources

Mapping single-nucleotide m6A by m6A-REF-seq

Methods, 2022
The past few years have witnessed rapid progress in the field of RNA modifications. As the most prevailing modification on eukaryotic mRNA, m6A is characterized to play a vital role in various cellular activities. However, limitations of the detection method impede functional studies of m6A. Here we introduce m6A-REF-seq, a powerful and straightforward
Hong-Xuan, Chen, Zhang, Zhang, Dong-Zhao, Ma, Li-Qian, Chen, Guan-Zheng, Luo   +4 more
openaire   +2 more sources

Nucleotide Analog Interference Mapping

2009
Nucleotide analog interference mapping (NAIM) is a powerful chemogenetic technique that rapidly identifies chemical groups essential for RNA function. Using a series of phosphorothioate-tagged nucleotide analogs, each carrying different modifications of nucleobase or backbone functionalities, it is possible to simultaneously, yet individually, assess ...
Ian T, Suydam, Scott A, Strobel
openaire   +2 more sources

Mapping oxidative DNA damage at nucleotide level

Free Radical Research, 1998
DNA damage induced by reactive oxygen species (ROS) is considered an important intermediate in the pathogenesis of human conditions such as cancer and aging. By developing an oxidative-induced DNA damage mapping version of the Ligation-mediated polymerase chain reaction (LMPCR) technique, we investigated the il vivo and in vitro frequencies of DNA base
H, Rodriguez, S A, Akman
openaire   +2 more sources

The use of single-nucleotide polymorphism maps in pharmacogenomics

Nature Biotechnology, 2000
Single-nucleotide polymorphisms (SNPs), common variations among the DNA of individuals, are being uncovered and assembled into large SNP databases that promise to enable the dissection of the genetic basis of disease and drug response (i.e., pharmacogenomics).
J J, McCarthy, R, Hilfiker
openaire   +2 more sources

Nucleotide Analog Interference Mapping

Cold Spring Harbor Protocols, 2015
Methods collectively known as modification interference are exceptionally powerful approaches used to identify functionally important chemical groups in the phosphodiester backbone or nucleobases of an RNA. In a modification interference assay, end-labeled RNAs that have been modified at different positions are allowed to participate in a reaction of ...
openaire   +2 more sources