Results 51 to 60 of about 262,017 (266)

Guidelines for Genotyping in Genomewide Linkage Studies: Single-Nucleotide–Polymorphism Maps Versus Microsatellite Maps [PDF]

The American Journal of Human Genetics, 2004
Genomewide linkage scans have traditionally employed panels of microsatellite markers spaced at intervals of approximately 10 cM across the genome. However, there is a growing realization that a map of closely spaced single-nucleotide polymorphisms (SNPs) may offer equal or superior power to detect linkage, compared with low-density microsatellite maps.
Evans, David M., Cardon, Lon R.
openaire   +3 more sources

The (Glg)ABCs of cyanobacteria: modelling of glycogen synthesis and functional divergence of glycogen synthases in Synechocystis sp. PCC 6803

FEBS Letters, EarlyView.
We reconstituted Synechocystis glycogen synthesis in vitro from purified enzymes and showed that two GlgA isoenzymes produce glycogen with different architectures: GlgA1 yields denser, highly branched glycogen, whereas GlgA2 synthesizes longer, less‐branched chains.
Kenric Lee, Dimitrios Bekiari, Sofia Doello, Karl Forchhammer   +3 more
wiley   +1 more source

Micro satellite mapping of plant genomes [PDF]

Selekcija i Semenarstvo, 2001
Micro satellites are DNA markers, based on the repeated nucleotide sequences number polymorphism. They belong to a group of PCR markers and are mainly used as an addition to other types of markers.
Prodanović Slaven, Živanović Tomislav, Dražić Slobodan   +2 more
doaj  

Development and diversity of Andean-derived, gene-based microsatellites for common bean (Phaseolus vulgaris L.)

BMC Plant Biology, 2009
Background Gene-based (genic) microsatellites are a useful tool for plant genetics and simple sequence repeat loci can often be found in coding regions of the genome. While EST sequencing can be used to discover genic microsatellites, direct screening of
Giraldo Martha C, Torres Monica, Blair Matthew W, Pedraza Fabio   +3 more
doaj   +1 more source

Identification of QTL for Plant Architecture and Flowering Performance Traits in a Multi-Environment Evaluation of a Petunia axillaris × P. exserta Recombinant Inbred Line Population

Horticulturae, 2022
Field performance of herbaceous annual plants is largely determined by plant architecture and flowering performance. A Petunia axillaris × P. exserta F7 recombinant inbred line population was grown in four field environments across the United States, and
QiuXia C. Chen, Ryan M. Warner
doaj   +1 more source

The planar cell polarity protein Vangl2 interacts with the PDZ‐domains of Scribble but not with a unique PDZ‐like domain in Inturned

FEBS Letters, EarlyView.
Structural and biochemical characterisations show that the planar cell polarity (PCP) protein Inturned harbours a unique PDZ‐like domain that does not bind canonical PDZ‐binding motifs (PBMs) like that of another PCP protein Vangl2. In contrast, the apical‐basal polarity protein Scribble contains four PDZ domains that bind Vangl2, but one PDZ domain ...
Stephan Wilmes, Jan Brysch, Carmen Gelze, Lilli Meier, Daniel Kümmel   +4 more
wiley   +1 more source

Integrated genomic and proteomic profiling reveals insights into chemoradiation resistance in cervical cancer

Molecular Oncology, EarlyView.
A comprehensive genomic and proteomic analysis of cervical cancer revealed STK11 and STX3 as a potential biomarkers of chemoradiation resistance. Our study demonstrated EGFR as a therapeutic target, paving the way for precision strategies to overcome treatment failure and the DNA repair pathway as a critical mechanism of resistance.
Janani Sambath, Irene A. George, Srikanth S. Manda, Prasanth Ariyannur, Ekta R. Dhawale, Raja Sekhar Kommu, Rajan Datar, Darshana Patil, Vinita Trivedi, Manisha Singh, Kumar Prabhash, Sewanti Limaye, Richa Chauhan, Prashant Kumar   +13 more
wiley   +1 more source

Modeling hepatic fibrosis in TP53 knockout iPSC‐derived human liver organoids

Molecular Oncology, EarlyView.
This study developed iPSC‐derived human liver organoids with TP53 gene knockout to model human liver fibrosis. These organoids showed elevated myofibroblast activation, early disease markers, and advanced fibrotic hallmarks. The use of profibrotic differentiation medium further amplified the fibrotic signature seen in the organoids.
Mustafa Karabicici, Soheil Akbari, Ceyda Caliskan, Canan Celiker, Ozden Oz, Leman Binokay, Gökhan Karakulah, Serif Senturk, Esra Erdal   +8 more
wiley   +1 more source

High throughput ANI analysis of 90K prokaryotic genomes reveals clear species boundaries

Nature Communications, 2018
Average Nucleotide Identity (ANI) is a robust and useful measure to gauge genetic relatedness between two genomes. Here, the authors develop FastANI, a method to compute ANI using alignment-free approximate sequence mapping, and show 95% ANI is an ...
Chirag Jain, Luis M. Rodriguez-R, Adam M. Phillippy, Konstantinos T. Konstantinidis, Srinivas Aluru   +4 more
doaj   +1 more source

A quantitative map of nucleotide substitution rates in bacterial rRNA [PDF]

Nucleic Acids Research, 1996
A recently developed method for estimating the variability of nucleotide sites in a sequence alignment [Van de Peer, Y., Van der Auwera, G. and De Wachter, R. (1996) J. Mol. Evol. 42, 201-210] was applied to bacterial 16S, 5S and 23S rRNAs. In this method, the variability of each nucleotide site is defined as its evolutionary rate relative to the ...
Van de Peer, Yves, Chapelle, Sabine, De Wachter, Rupert   +2 more
openaire   +3 more sources