Results 41 to 50 of about 270,206 (261)

Population structure and association mapping studies for yield-related traits in Maize (Zea mays L.)

Current Plant Biology, 2019
Association mapping is an efficient approach for dissecting the genetic architecture of yield-related traits using single nucleotide polymorphism (SNP) markers in maize. The present study was carried out with a set of 93 diverse maize accessions with the
S. Sivakumar, M. Dhasarathan, A. Karthikeyan, P. Bharathi, N. Kumari vinodhana, K. Ganesamurthy, N. Senthil   +6 more
doaj   +1 more source

Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation

FEBS Letters, EarlyView.
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe, Tanmay Kumar Mohanty, Rashna Bhandari, Dorothea Fiedler   +3 more
wiley   +1 more source

Mapping ribonucleotides embedded in genomic DNA to single-nucleotide resolution using Ribose-Map [PDF]

Nature Protocols, 2020
ABSTRACT Ribose-Map is a user-friendly, standardized bioinformatics toolkit for the comprehensive analysis of ribonucleotide sequencing experiments. It allows researchers to map the locations of ribonucleotides in DNA to single-nucleotide resolution and identify biological signatures of ribonucleotide incorporation.
Alli L. Gombolay, Francesca Storici
openaire   +2 more sources

The planar cell polarity protein Vangl2 interacts with the PDZ‐domains of Scribble but not with a unique PDZ‐like domain in Inturned

FEBS Letters, EarlyView.
Structural and biochemical characterisations show that the planar cell polarity (PCP) protein Inturned harbours a unique PDZ‐like domain that does not bind canonical PDZ‐binding motifs (PBMs) like that of another PCP protein Vangl2. In contrast, the apical‐basal polarity protein Scribble contains four PDZ domains that bind Vangl2, but one PDZ domain ...
Stephan Wilmes, Jan Brysch, Carmen Gelze, Lilli Meier, Daniel Kümmel   +4 more
wiley   +1 more source

High-throughput isolation and mapping of C. elegans mutants susceptible to pathogen infection. [PDF]

PLoS ONE, 2008
We present a novel strategy that uses high-throughput methods of isolating and mapping C. elegans mutants susceptible to pathogen infection. We show that C.
Laura E Fuhrman, Kevin V Shianna, Alejandro Aballay   +2 more
doaj   +1 more source

Single-nucleotide-resolution mapping of m6A and m6Am throughout the transcriptome [PDF]

Nature Methods, 2015
N(6)-methyladenosine (m6A) is the most abundant modified base in eukaryotic mRNA and has been linked to diverse effects on mRNA fate. Current mapping approaches localize m6A residues to transcript regions 100-200 nt long but cannot identify precise m6A positions on a transcriptome-wide level. Here we developed m6A individual-nucleotide-resolution cross-
Linder, Bastian, Grozhik, Anya V., Olarerin-George, Anthony O., Meydan, Cem, Mason, Christopher E., Jaffrey, Samie R.   +5 more
openaire   +2 more sources

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source

Genome-wide mapping of nucleotide excision repair with XR-seq [PDF]

Nature Protocols, 2018
Nucleotide excision repair is a versatile mechanism to repair a variety of bulky DNA adducts. We developed excision repair sequencing (XR-seq) to study nucleotide excision repair of DNA adducts in humans, mice, Arabidopsis thaliana, yeast and Escherichia coli. In this protocol, the excised oligomers, generated in the nucleotide excision repair reaction,
Jinchuan Hu, Wentao Li, Ogun Adebali, Yanyan Yang, Onur Oztas, Christopher P. Selby, Aziz Sancar   +6 more
openaire   +4 more sources

Diversity and complexity in neural organoids

FEBS Letters, EarlyView.
Neural organoid research aims to expand genetic diversity on one side and increase tissue complexity on the other. Chimeroids integrate multiple donor genomes within single organoids. Self‐organising multi‐identity organoids, exogenous cell seeding, or enforced assembly of region‐specific organoids contribute to tissue complexity.
Ilaria Chiaradia, Madeline A. Lancaster
wiley   +1 more source

Precise and Cost-Effective Nanopore Sequencing for Post-GWAS Fine-Mapping and Causal Variant Identification

iScience, 2020
Summary: Fine-mapping of interesting loci discovered by genome-wide association study (GWAS) is mandatory to pinpoint causal variants. Traditionally, this fine-mapping is completed through increasing the genotyping density at candidate loci, for which ...
Tarek Magdy, Hui–Hsuan Kuo, Paul W. Burridge   +2 more
doaj   +1 more source