Kinetic analysis of rabbit liver tRNA nucleotidyltransferase.
Journal of Biological Chemistry, 1978 The kinetic properties of rabbit liver tRNA nucleotidyltransferase were examined. Our results indicate that incorporation of AMP into tRNA-C-C at pH 9.4 proceeds by a rapid equilibrium Random Bi Bi mechanism. This conclusion was supported by bisubstrate initial velocity studies, dead end and product inhibition, and isotope exchange at equilibrium and ...
J A, Evans, M P, Deutscher
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Viroids: Survivors from the RNA World? [PDF]
, 2014 Because RNA can be a carrier of genetic information and a biocatalyst, there is a consensus that it emerged before DNA and proteins, which eventually assumed these roles and relegated RNA to intermediate functions.
Flores, Ricardo +3 more
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Molecular dissection of mRNA poly(A) tail length control in yeast [PDF]
, 2008 In eukaryotic cells, newly synthesized mRNAs acquire a poly(A) tail that plays several fundamental roles in export, translation and mRNA decay. In mammals, PABPN1 controls the processivity of polyadenylation and the length of poly(A) tails during de novo
Afonina +53 more
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Solution, solid-phase, and fluorous-phase synthesis of carbohydrates and production of carbohydrate microarrays [PDF]
, 2006 This dissertation focuses on two specific aspects of the chemistry of carbohydrates. The first is the solid- and fluorous-phase synthesis of oligosaccharides and their use in forming microarrays.
Ko, Kwang-Seuk
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EVALUATION OF ANTIBIOTIC RESISTANCE PROFILES OF HALOBACTERIA ISOLATED FROM THE FOOD CHAIN [PDF]
, 2017 L’insorgenza e la diffusione dell’antibiotico resistenza sta diventando un problema a livello mondiale. Molti sono gli ambienti in cui può avvenire tale diffusione, ma una delle principali vie di trasmissione passa attraverso la catena alimentare ...
FALASCONI, IRENE
core
Probing carbohydrate biocatalysis through the use of electrospray ionization mass spectrometry and isothermal titration calorimetry [PDF]
, 2005 Electrospray ionization mass spectrometry,(ESI-MS)-based assays have been newly developed to study three classes of enzymes (sugar nucleotidyltransferases, glycosyltransferases, and phosphorylases) from the three distinct families of life: archaea ...
Zea, Corbin James
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Expression, Enzymatic Activities and Subcellular Localization of Hepatitis E Virus and Semliki Forest Virus Replicase Proteins [PDF]
, 2006 Viruses are submicroscopic, infectious agents that are obligate intracellular parasites. They adopt various types of strategies for their parasitic replication and proliferation in infected cells.
Perttilä, Julia
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A human mitochondrial poly(A) polymerase mutation reveals the complexities of post-transcriptional mitochondrial gene expression [PDF]
, 2017 The p.N478D missense mutation in human mitochondrial poly(A) polymerase (mtPAP) has previously been implicated in a form of spastic ataxia with optic atrophy. In this study, we have investigated fibroblast cell lines established from family members.
Baslé, Arnaud +17 more
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Biophysical and biochemical characterization of yeast tRNA nucleotidyltransferase variants [PDF]
, 2017 The enzyme ATP(CTP):tRNA-specific tRNA nucleotidyltransferase adds cytidine-cytidine-adenosine (CCA) to the 3’ end of eukaryotic tRNAs during their maturation. This CCA sequence plays a vital role in aminoacylation and hence in protein synthesis.
Rahman, Mohammed Samiur
core