Results 1 to 10 of about 4,883 (125)

Primer- and template-independent RNA polymerization by terminal nucleotidyltransferase TENT4B [PDF]

Nucleic Acids Res
Abstract RNA synthesis by eukaryotic polymerases requires existing polynucleotides to serve as templates or primers. Here, we describe primer- and template-free RNA generation by human terminal nucleotidyltransferase 4B (TENT4B) via de novo polymerization of free nucleotides.
Neha Nagpal, Albert K. Tai, Yick W. Fong, Suneet Agarwal   +3 more
openaire   +3 more sources

Microbial profile of the appendix niche in acute appendicitis: a novel sampling approach

FEBS Open Bio, Volume 15, Issue 12, Page 2001-2020, December 2025.
This study utilized a novel sampling method, ERAT (i.e. endoscopic retrograde appendicitis treatment)‐guided lumen aspiration, to obtain samples from the appendix, and shotgun metagenomic sequencing was performed for in situ characterization of the appendix microbiome in patients with acute appendicitis.
Huimin Ma, Mingfei Wang, Yanhu Feng, Wanqi Zhang, Wenbo Wang, Yanfeng Xie, Guixiang Kong, Jie Feng, Pengfei Wang, Qi Wang, Xiaojun Huang   +10 more
wiley   +1 more source

Transfer RNA pyrophosphorolysis with CTP(ATP):tRNA nucleotidyltransferase. A direct route to tRNAs modified at the 3' terminus.

Journal of Biological Chemistry, 1983
The pyrophosphorolysis of tRNA by yeast CTP-(ATP):tRNA nucleotidyltransferase has been studied in an effort to define the behavior of the enzyme and the experimental parameters that lead to net loss of the 3'-terminal nucleotide or to nucleotide exchange.
T A, Francis, G M, Ehrenfeld, M R, Gregory, S M, Hecht   +3 more
openaire   +2 more sources

Recognition of TMV RNA by the tRNA nucleotidyltransferase [PDF]

FEBS Letters, 1974
Benicourt, Claude, Haenni, Anne-Lise
openaire   +2 more sources

A novel function of Escherichia coli transfer RNA nucleotidyltransferase. Biosynthesis of the C-C-A sequence in a phage T4 transfer RNA precursor.

Journal of Biological Chemistry, 1975
The biosynthesis of the phage T4-coded proline and serine transfer RNA species proceeds through a precursor RNA containing both tRNA sequences. Neither tRNA sequence in the precursor RNA contains the 3'-terminal C-C-A common to all mature tRNAs. Seidman and McClain ((1975) Proc. Natl. Acad. Sci. U. S. A.
openaire   +2 more sources

Biochemical and structural characterization of the RlaP family nucleotidyltransferase potentially involved in RNA repair

Abstract Nucleotidyltransferases (NTases) of Polβ superfamily are highly diverse and play many important biological functions. However, many families of NTases remain uncharacterized. Because of physical proximity of the genes encoding NTases of PF10127 family to those encoding RNA ligases involved in RNA repair ...
Carruthers, Amy, Fatma, Shirin, Huang, Raven H.   +2 more
openaire   +2 more sources

Expanding the Available RNA Labeling Toolbox With CutA Nucleotidyltransferase for Efficient Transcript Labeling with Purine and Pyrimidine Nucleotide Analogs

ChemBioChem
AbstractRNA labeling is an invaluable tool for investigation of the function and localization of nucleic acids. Labels are commonly incorporated into 3′ end of RNA and the primary enzyme used for this purpose is RNA poly(A) polymerase (PAP), which belongs to the class of terminal nucleotidyltransferases (NTases).
Rafal Tomecki, Karolina Drazkowska, Rafal Madaj, Adam Mamot, Stanislaw Dunin‐Horkawicz, Pawel J. Sikorski   +5 more
openaire   +4 more sources

Phylogeny and Evolution of RNA 3′-Nucleotidyltransferases in Bacteria

Journal of Molecular Evolution, 2019
The tRNA nucleotidyltransferases and poly(A) polymerases belong to a superfamily of nucleotidyltransferases. The amino acid sequences of a number of bacterial tRNA nucleotidyltransferases and poly(A) polymerases have been used to construct a rooted, neighbor-joining phylogenetic tree.
George Jones
exaly   +3 more sources

The polynucleotide ligase and RNA capping enzyme superfamily of covalent nucleotidyltransferases

Current Opinion in Structural Biology, 2004
ATP- and NAD(+)-dependent DNA ligases, ATP-dependent RNA ligases and GTP-dependent mRNA capping enzymes comprise a superfamily of proteins that catalyze nucleotidyl transfer to polynucleotide 5' ends via covalent enzyme-(lysyl-N)-NMP intermediates. The superfamily is defined by five peptide motifs that line the nucleotide-binding pocket and contribute ...
Stewart Shuman, Christopher D Lima
exaly   +3 more sources

Transfer RNA nucleotidyltransferase repairs all transfer RNAs randomly

Journal of Molecular Biology, 1977
Total Escherichia coli transfer RNA was converted to tRNA-C-C by periodate oxidation and partially repaired with either E. coli or rabbit liver tRNA nucleotidyltransferase. Aminoacylation of the partially repaired molecules indicated that individual tRNA species were each repaired to the same extent as the total population of molecules.
M P, Deutscher, J A, Evans
openaire   +2 more sources