Results 21 to 30 of about 4,883 (125)

tRNA‐nucleotidyltransferases: Highly unusual RNA polymerases with vital functions [PDF]

FEBS Letters, 2009
tRNA‐nucleotidyltransferases are fascinating and unusual RNA polymerases responsible for the synthesis of the nucleotide triplet CCA at the 3′‐terminus of tRNAs. As this CCA end represents an essential functional element for aminoacylation and translation, these polymerases (CCA‐adding enzymes) are of vital importance in all organisms.
Vörtler, Stefan, Mörl, Mario
openaire   +2 more sources

Reproducible and efficient new method of RNA 3’-end labelling by CutA nucleotidyltransferase-mediated CC-tailing [PDF]

RNA Biology, 2021
Despite the development of non-radioactive DNA/RNA labelling methods, radiolabelled nucleic acids are commonly used in studies focused on the determination of RNA fate. Nucleic acid fragments with radioactive nucleotide analoguesincorporated into the body or at the 5ʹ or 3ʹ terminus of the molecule can serve as probes in hybridization-based analyses of
Rafal Tomecki, Kamil Kobylecki, Karolina Drazkowska, Malwina Hyjek-Skladanowska, Andrzej Dziembowski   +4 more
openaire   +3 more sources

Nontemplated Terminal Nucleotidyltransferase Activity of Double-Stranded RNA Bacteriophage φ6 RNA-Dependent RNA Polymerase [PDF]

Journal of Virology, 2008
ABSTRACT The replication and transcription of double-stranded RNA (dsRNA) viruses occur within a polymerase complex particle in which the viral genome is enclosed throughout the entire life cycle of the virus. A single protein subunit in the polymerase complex is responsible for the template-dependent RNA polymerization activity. The isolated
Minna M, Poranen, Minni R L, Koivunen, Dennis H, Bamford   +2 more
openaire   +2 more sources

U2 Small Nuclear RNA Is a Substrate for the CCA-adding Enzyme (tRNA Nucleotidyltransferase) [PDF]

Journal of Biological Chemistry, 2002
The CCA-adding enzyme builds and repairs the 3' terminus of tRNA. Approximately 65% of mature human U2 small nuclear RNA (snRNA) ends in 3'-terminal CCA, as do all mature tRNAs; the other 35% ends in 3' CC or possibly 3' C. The 3'-terminal A of U2 snRNA cannot be encoded because the 3' end of the U2 snRNA coding region is CC/CC, where the slash ...
HyunDae D, Cho, Kozo, Tomita, Tsutomu, Suzuki, Alan M, Weiner   +3 more
openaire   +2 more sources

In silico investigation to identify potential small molecule inhibitors of the RNA-dependent RNA polymerase (RdRp) nidovirus RdRp-associated nucleotidyltransferase domain

Chemical Physics Letters, 2021
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA-dependent RNA polymerase (RdRp) is a promising target for antiviral drugs. In this study, a chemical library (n = 300) was screened against the nidovirus RdRp-associated nucleotidyltransferase (NiRAN) domain.
Eleni Pitsillou, Julia Liang, Helen Yu Meng Huang, Andrew Hung, Tom C. Karagiannis   +4 more
openaire   +2 more sources

Translocation and Rotation of tRNA during Template-Independent RNA Polymerization by tRNA Nucleotidyltransferase [PDF]

Structure, 2014
The 3'-terminal CCA (CCA-3' at positions 74-76) of tRNA is synthesized by CCA-adding enzyme using CTP and ATP as substrates, without a nucleic acid template. In Aquifex aeolicus, CC-adding and A-adding enzymes collaboratively synthesize the CCA-3'. The mechanism of CCA-3' synthesis by these two enzymes remained obscure.
Yamashita, Seisuke, Takeshita, Daijiro, Tomita, Kozo   +2 more
openaire   +2 more sources

A Member of the Polymerase β Nucleotidyltransferase Superfamily Is Required for RNA Interference in C. elegans [PDF]

Current Biology, 2005
RNA interference (RNAi) is an ancient, highly conserved mechanism in which small RNA molecules (siRNAs) guide the sequence-specific silencing of gene expression . Several silencing machinery protein components have been identified, including helicases, RNase-related proteins, double- and single-stranded RNA binding proteins, and RNA-dependent RNA ...
Chen, Chun-Chieh G., Simard, Martin J., Tabara, Hiroaki, Brownell, Daniel R., McCollough, Jennifer A., Mello, Craig C.   +5 more
openaire   +3 more sources

Characterization of a Nodavirus Replicase Revealed a de Novo Initiation Mechanism of RNA Synthesis and Terminal Nucleotidyltransferase Activity [PDF]

Journal of Biological Chemistry, 2013
Nodaviruses are a family of positive-stranded RNA viruses with a bipartite genome of RNAs. In nodaviruses, genomic RNA1 encodes protein A, which is recognized as an RNA-dependent RNA polymerase (RdRP) and functions as the sole viral replicase protein responsible for its RNA replication.
Zhaowei, Wang, Yang, Qiu, Yongxiang, Liu, Nan, Qi, Jie, Si, Xiaoling, Xia, Di, Wu, Yuanyang, Hu, Xi, Zhou   +8 more
openaire   +2 more sources

Detecting Antibiotic Resistance in Strains of Selected Gram‐Negative Bacterial Species Using Raman Spectroscopy

Journal of Raman Spectroscopy, Volume 57, Issue 9, Page 1554-1564, September 2026.
Raman spectroscopy successfully identified antibiotic‐resistant Gram‐negative strains. Resistant strains of four bacterial species were discriminated from sensitive ones with 99.1% accuracy using PLS regression. The method allows for rapid, label‐free detection of antibiotic resistance.
Fernanda Sant Ana de Siqueira e Oliveira, Adriano Moraes da Silva, Marcos Tadeu T. Pacheco, Landulfo Silveira Jr.   +3 more
wiley   +1 more source

Manipulation of mitochondrial poly(A) polymerase family proteins in Trypanosoma brucei impacts mRNA termini processing

Frontiers in Parasitology
RNA-specific nucleotidyltransferases (rNTrs) add nontemplated nucleotides to the 3′ end of RNA. Two noncanonical rNTRs that are thought to be poly(A) polymerases (PAPs) have been identified in the mitochondria of trypanosomes – KPAP1 and KPAP2.
Clara M. Smoniewski, Poorya Mirzavand Borujeni, Marshall Hampton, Austin Petersen, Sean P. Faacks, Reza Salavati, Sara L. Zimmer   +6 more
doaj   +1 more source