Results 21 to 30 of about 5,405 (175)

Primase-polymerases are a functionally diverse superfamily of replication and repair enzymes [PDF]

, 2015
Until relatively recently, DNA primases were viewed simply as a class of proteins that synthesize short RNA primers requisite for the initiation of DNA replication.
Aidan J. Doherty, Benjamin A. Keen, Bianchi, Bouché, Collins, Crute, Kaguni, Klinedinst, Klinedinst, Koonin, Marsden, Moss, Nigel C. Brissett, Rowen, Scherzinger, Sherman, Takechi, Thomas A. Guilliam   +17 more
core   +1 more source

Physicochemical analysis of rotavirus segment 11 supports a 'modified panhandle' structure and not the predicted alternative tRNA-like structure (TRLS) [PDF]

, 2013
.Rotaviruses are a major cause of acute gastroenteritis, which is often fatal in infants. The viral genome consists of 11 double-stranded RNA segments, but little is known about their cis-acting sequences and structural elements.
A Heerschap, A L’Hernault, A Wilm, AE Simon, Andrew M. Lever, AR Gruber, BI Klasens, D Chen, D Chen, D Yue, D Yue, E Madore, E Matsuo, EC Hutchinson, Emily Manktelow, EV Puglisi, H Heus, HD Cho, J Jia, JC Kenyon, Jonathan Lever, JR Gog, JT Patton, Laura E. Easton, LE Easton, M Barro, M Estes, MA Tortorici, MW Friederich, N Figueroa, P Annamalai, Peter J. Lukavsky, PJ Lukavsky, R Giegé, R Giegé, RC Olsthoorn, S Nishimura, SC Chen, SH Bernhart, Subhajit Biswas, T Nagaike, TW Dreher, TW Dreher, TW Dreher, TW Dreher, Ulrich Desselberger, W Li, Wilson Li   +47 more
core   +1 more source

In silico investigation to identify potential small molecule inhibitors of the RNA-dependent RNA polymerase (RdRp) nidovirus RdRp-associated nucleotidyltransferase domain

Chemical Physics Letters, 2021
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA-dependent RNA polymerase (RdRp) is a promising target for antiviral drugs. In this study, a chemical library (n = 300) was screened against the nidovirus RdRp-associated nucleotidyltransferase (NiRAN) domain.
Eleni Pitsillou, Julia Liang, Helen Yu Meng Huang, Andrew Hung, Tom C. Karagiannis   +4 more
openaire   +2 more sources

Relating the metatranscriptome and metagenome of the human gut [PDF]

, 2014
Although the composition of the human microbiome is now wellstudied, the microbiota’s \u3e8 million genes and their regulation remain largely uncharacterized.
Boylan, Matthew R., Chan, Andrew T., Ciulla, Dawn, Earl, Ashlee M., Franzosa, Eric A., Garrett, Wendy S., Gevers, Dirk, Giannoukos, Georgia, Huttenhower, Curtis, Izard, Jacques, Morgan, Xochitl C., Reyes, Joshua, Segata, Nicola, Waldron, Levi   +13 more
core   +2 more sources

Translocation and Rotation of tRNA during Template-Independent RNA Polymerization by tRNA Nucleotidyltransferase [PDF]

Structure, 2014
The 3'-terminal CCA (CCA-3' at positions 74-76) of tRNA is synthesized by CCA-adding enzyme using CTP and ATP as substrates, without a nucleic acid template. In Aquifex aeolicus, CC-adding and A-adding enzymes collaboratively synthesize the CCA-3'. The mechanism of CCA-3' synthesis by these two enzymes remained obscure.
Yamashita, Seisuke, Takeshita, Daijiro, Tomita, Kozo   +2 more
openaire   +2 more sources

The NF45/NF90 Heterodimer Contributes to the Biogenesis of 60S Ribosomal Subunits and Influences Nucleolar Morphology [PDF]

, 2015
The interleukin enhancer binding factors ILF2 (NF45) and ILF3 (NF90/NF110) have been implicated in various cellular pathways, such as transcription, microRNA (miRNA) processing, DNA repair, and translation, in mammalian cells.
Badertscher, Lukas, Bammert, Lukas, Horváth, Péter, Koós, Krisztián, Montellese, Christian, Wandrey, Franziska   +5 more
core   +1 more source

U2 Small Nuclear RNA Is a Substrate for the CCA-adding Enzyme (tRNA Nucleotidyltransferase) [PDF]

Journal of Biological Chemistry, 2002
The CCA-adding enzyme builds and repairs the 3' terminus of tRNA. Approximately 65% of mature human U2 small nuclear RNA (snRNA) ends in 3'-terminal CCA, as do all mature tRNAs; the other 35% ends in 3' CC or possibly 3' C. The 3'-terminal A of U2 snRNA cannot be encoded because the 3' end of the U2 snRNA coding region is CC/CC, where the slash ...
HyunDae D, Cho, Kozo, Tomita, Tsutomu, Suzuki, Alan M, Weiner   +3 more
openaire   +2 more sources

Comprehensive analysis of the HEPN superfamily: identification of novel roles in intra-genomic conflicts, defense, pathogenesis and RNA processing [PDF]

, 2013
BACKGROUND: The major role of enzymatic toxins that target nucleic acids in biological conflicts at all levels has become increasingly apparent thanks in large part to the advances of comparative genomics.
A Maxwell Burroughs, Eugene V Koonin, Kira S Makarova, L Aravind, Vivek Anantharaman   +4 more
core   +1 more source

A Member of the Polymerase β Nucleotidyltransferase Superfamily Is Required for RNA Interference in C. elegans [PDF]

Current Biology, 2005
RNA interference (RNAi) is an ancient, highly conserved mechanism in which small RNA molecules (siRNAs) guide the sequence-specific silencing of gene expression . Several silencing machinery protein components have been identified, including helicases, RNase-related proteins, double- and single-stranded RNA binding proteins, and RNA-dependent RNA ...
Chen, Chun-Chieh G., Simard, Martin J., Tabara, Hiroaki, Brownell, Daniel R., McCollough, Jennifer A., Mello, Craig C.   +5 more
openaire   +3 more sources

Two Classes of Silencing RNAs Move between Caenorhabditis elegans Tissues [PDF]

, 2013
Organism-wide RNA interference (RNAi) is due to the transport of mobile silencing RNA throughout the organism, but the identities of these mobile RNA species in animals are unknown. Here, we present genetic evidence that both the initial double-stranded
Garcia, Giancarlo, Hunter, Craig P., Jose, Antony Merlin   +2 more
core   +1 more source