Results 21 to 30 of about 4,731 (160)

Translocation and Rotation of tRNA during Template-Independent RNA Polymerization by tRNA Nucleotidyltransferase [PDF]

Structure, 2014
The 3'-terminal CCA (CCA-3' at positions 74-76) of tRNA is synthesized by CCA-adding enzyme using CTP and ATP as substrates, without a nucleic acid template. In Aquifex aeolicus, CC-adding and A-adding enzymes collaboratively synthesize the CCA-3'. The mechanism of CCA-3' synthesis by these two enzymes remained obscure.
Yamashita, Seisuke, Takeshita, Daijiro, Tomita, Kozo   +2 more
openaire   +2 more sources

In silico investigation to identify potential small molecule inhibitors of the RNA-dependent RNA polymerase (RdRp) nidovirus RdRp-associated nucleotidyltransferase domain

Chemical Physics Letters, 2021
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA-dependent RNA polymerase (RdRp) is a promising target for antiviral drugs. In this study, a chemical library (n = 300) was screened against the nidovirus RdRp-associated nucleotidyltransferase (NiRAN) domain.
Eleni Pitsillou, Julia Liang, Helen Yu Meng Huang, Andrew Hung, Tom C. Karagiannis   +4 more
openaire   +2 more sources

Analysis of Unusual Eukaryotic tRNA Nucleotidyltransferases and Establishment of a High-Throughput Sequencing Method for Mature tRNAs

, 2020
Transfer RNA nucleotidyltransferases (CCA-adding enzymes) are important enzymes, which catalyze the attachment of a CCA triplet to the 3‘ end of tRNAs, an essential requirement for subsequent aminoacylation.
Erber, Lieselotte
core   +1 more source

A Member of the Polymerase β Nucleotidyltransferase Superfamily Is Required for RNA Interference in C. elegans [PDF]

Current Biology, 2005
RNA interference (RNAi) is an ancient, highly conserved mechanism in which small RNA molecules (siRNAs) guide the sequence-specific silencing of gene expression . Several silencing machinery protein components have been identified, including helicases, RNase-related proteins, double- and single-stranded RNA binding proteins, and RNA-dependent RNA ...
Chen, Chun-Chieh G., Simard, Martin J., Tabara, Hiroaki, Brownell, Daniel R., McCollough, Jennifer A., Mello, Craig C.   +5 more
openaire   +3 more sources

Biochemical characterization and three-dimensional structure analysis of the yeast cleavage and polyadenylation factor CPF [PDF]

, 2008
In eukaryotes, protein-encoding genes are transcribed in the nucleus by RNA polymerase II (RNAP II). Before the gene transcript (pre-mRNA) is transported to the cytoplasm and can function in translation, it has to undergo three specific maturation ...
Paguet, Bertrand
core   +1 more source

U2 Small Nuclear RNA Is a Substrate for the CCA-adding Enzyme (tRNA Nucleotidyltransferase) [PDF]

Journal of Biological Chemistry, 2002
The CCA-adding enzyme builds and repairs the 3' terminus of tRNA. Approximately 65% of mature human U2 small nuclear RNA (snRNA) ends in 3'-terminal CCA, as do all mature tRNAs; the other 35% ends in 3' CC or possibly 3' C. The 3'-terminal A of U2 snRNA cannot be encoded because the 3' end of the U2 snRNA coding region is CC/CC, where the slash ...
HyunDae D, Cho, Kozo, Tomita, Tsutomu, Suzuki, Alan M, Weiner   +3 more
openaire   +2 more sources

Histone mRNA is subject to 3’ uridylation and re-adenylation in Aspergillus nidulans [PDF]

, 2021
The role of post-transcriptional RNA modification is of growing interest. One example is the addition of non-templated uridine residues to the 3´ end of transcripts.
Mark X. Caddick, Morozov, Igor; id_orcid, Caddick, MX, Caddick, Mark X., Daniele Parisi, Olga Mayans, Browne-Marke, N, Natasha Browne‐Marke, Paola Fucini, Morozov, IY, Fucini, P, Igor Y. Morozov, Parisi, D, Mossanen-Parsi, Amir, Browne-Marke, Natasha, Mayans, Olga, Connell, SR, Sean R. Connell, Bharudin, I, Bharudin, Izwan, Parisi, Daniele, Fucini, Paola, Mossanen-Parsi, A, Connell, Sean R., Mayans, O, Izwan Bharudin, Morozov, Igor Y., Amir Mossanen‐Parsi   +27 more
core   +1 more source

Characterization of a Nodavirus Replicase Revealed a de Novo Initiation Mechanism of RNA Synthesis and Terminal Nucleotidyltransferase Activity [PDF]

Journal of Biological Chemistry, 2013
Nodaviruses are a family of positive-stranded RNA viruses with a bipartite genome of RNAs. In nodaviruses, genomic RNA1 encodes protein A, which is recognized as an RNA-dependent RNA polymerase (RdRP) and functions as the sole viral replicase protein responsible for its RNA replication.
Zhaowei, Wang, Yang, Qiu, Yongxiang, Liu, Nan, Qi, Jie, Si, Xiaoling, Xia, Di, Wu, Yuanyang, Hu, Xi, Zhou   +8 more
openaire   +2 more sources

Characterization of factors involved in the coupling of 3' end processing and splicing and in the 3' end formation of mRNA precursors [PDF]

, 2006
Eukaryotic mRNA precursors are processed at their 5’ and 3’ ends and are spliced prior to their export from the nucleus to the cytoplasm. Although all three processing reactions can be studied separately in vitro, they are coupled in vivo.
Kyburz Kooznetsoff, Andrea Martina
core   +1 more source

Manipulation of mitochondrial poly(A) polymerase family proteins in Trypanosoma brucei impacts mRNA termini processing

Frontiers in Parasitology
RNA-specific nucleotidyltransferases (rNTrs) add nontemplated nucleotides to the 3′ end of RNA. Two noncanonical rNTRs that are thought to be poly(A) polymerases (PAPs) have been identified in the mitochondria of trypanosomes – KPAP1 and KPAP2.
Clara M. Smoniewski, Poorya Mirzavand Borujeni, Marshall Hampton, Austin Petersen, Sean P. Faacks, Reza Salavati, Sara L. Zimmer   +6 more
doaj   +1 more source