Results 11 to 20 of about 5,405 (175)
A phylogeny of bacterial RNA nucleotidyltransferases: Bacillus halodurans contains two tRNA nucleotidyltransferases. [PDF]
J Bacteriol, 2005 ABSTRACT We have analyzed the distribution of RNA nucleotidyltransferases from the family that includes poly(A) polymerases (PAP) and tRNA nucleotidyltransferases (TNT) in 43 bacterial species. Genes of several bacterial species encode only one member of the nucleotidyltransferase superfamily (NTSF), and if that protein functions
Bralley P, Chang SA, Jones GH.
europepmc +6 more sources
tRNA‐nucleotidyltransferases: Highly unusual RNA polymerases with vital functions [PDF]
FEBS Letters, 2009 tRNA‐nucleotidyltransferases are fascinating and unusual RNA polymerases responsible for the synthesis of the nucleotide triplet CCA at the 3′‐terminus of tRNAs. As this CCA end represents an essential functional element for aminoacylation and translation, these polymerases (CCA‐adding enzymes) are of vital importance in all organisms.
Vörtler, Stefan, Mörl, Mario
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RNA minihelices as model substrates for ATP/CTP:tRNA nucleotidyltransferase [PDF]
Biochemical Journal, 1997 Twenty-one RNA minihelices, resembling the coaxially stacked acceptor- /T-stems and T-loop found along the top of a tRNA's three-dimensional structure, were synthesized and used as substrates for ATP/CTP:tRNA nucleotidyltransferases from Escherichia coli and Saccharomyces cerevisiae.
Z, Li, Y, Sun, D L, Thurlow
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Functions and mechanisms of
WIREs RNA, 2020 AbstractTermini often determine the fate of RNA molecules. In recent years, 3′ ends of almost all classes of RNA species have been shown to acquire nontemplated nucleotides that are added by terminal nucleotidyltransferases (TENTs). The best‐described role of 3′ tailing is the bulk polyadenylation of messenger RNAs in the cell nucleus that is catalyzed
Liudkovska, Vladyslava +1 more
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Nontemplated Terminal Nucleotidyltransferase Activity of Double-Stranded RNA Bacteriophage φ6 RNA-Dependent RNA Polymerase [PDF]
Journal of Virology, 2008 ABSTRACT The replication and transcription of double-stranded RNA (dsRNA) viruses occur within a polymerase complex particle in which the viral genome is enclosed throughout the entire life cycle of the virus. A single protein subunit in the polymerase complex is responsible for the template-dependent RNA polymerization activity. The isolated
Minna M, Poranen +2 more
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TYMV RNA As a substrate of the tRNA nucleotidyltransferase [PDF]
FEBS Letters, 1970 It has been recently shown that in the presence of partly purified enzyme preparations from Escherichia coli and ATP. lhe RNA (M.W. = 2 × 106) of Turnip Yellow Mosaic Virus (TYMV) reacts with valine forming an ester bond analogous to that existing in aminoacyltRNA: lhe valine estcrifies the., -terminal adenosine ofTYMV RNA II. 21.
Litvak, S., Carré, D.S., Chapeville, F.
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Reproducible and efficient new method of RNA 3’-end labelling by CutA nucleotidyltransferase-mediated CC-tailing [PDF]
RNA Biology, 2021 Despite the development of non-radioactive DNA/RNA labelling methods, radiolabelled nucleic acids are commonly used in studies focused on the determination of RNA fate. Nucleic acid fragments with radioactive nucleotide analoguesincorporated into the body or at the 5ʹ or 3ʹ terminus of the molecule can serve as probes in hybridization-based analyses of
Rafal Tomecki +4 more
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Mechanism of Mg 2+ -accompanied product release in sugar nucleotidyltransferases [PDF]
, 2018 The nucleotidyl transfer reaction, catalyzed by sugar nucleotidyltransferases (SNTs), is assisted by two active site Mg 2+ ions. While studying this reaction using X-ray crystallography, we captured snapshots of the pyrophosphate (product) as it exits ...
Awasthi, Shalini +6 more
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NF45 dimerizes with NF90, Zfr and SPNR via a conserved domain that has a nucleotidyltransferase fold [PDF]
, 2012 Nuclear factors NF90 and NF45 form a complex involved in a variety of cellular processes and are thought to affect gene expression both at the transcriptional and translational level.
Adams +47 more
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The influence of spermidine on the reaction of RNA nucleotidyltransferase
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1968 Abstract 1. 1. RNA synthesis, catalyzed by RNA nucleotidyltransferase ( Escherichia coli B) and directed by native, double-stranded DNA, is significantly increased by spermidine. 2. 2. By use of doubly labeled ATP ([8- 14 C]- and [γ- 32 P]-) and by zone centrifugation, we could show that, in the presence of spermidine, more RNA chains are ...
Petersen, E. E., Kroeger, H., Hagen, U.
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