Results 11 to 20 of about 4,731 (160)

tRNA‐nucleotidyltransferases: Highly unusual RNA polymerases with vital functions [PDF]

FEBS Letters, 2009
tRNA‐nucleotidyltransferases are fascinating and unusual RNA polymerases responsible for the synthesis of the nucleotide triplet CCA at the 3′‐terminus of tRNAs. As this CCA end represents an essential functional element for aminoacylation and translation, these polymerases (CCA‐adding enzymes) are of vital importance in all organisms.
Vörtler, Stefan, Mörl, Mario
openaire   +3 more sources

Reproducible and efficient new method of RNA 3’-end labelling by CutA nucleotidyltransferase-mediated CC-tailing [PDF]

RNA Biology, 2021
Despite the development of non-radioactive DNA/RNA labelling methods, radiolabelled nucleic acids are commonly used in studies focused on the determination of RNA fate. Nucleic acid fragments with radioactive nucleotide analoguesincorporated into the body or at the 5ʹ or 3ʹ terminus of the molecule can serve as probes in hybridization-based analyses of
Rafal Tomecki, Kamil Kobylecki, Karolina Drazkowska, Malwina Hyjek-Skladanowska, Andrzej Dziembowski   +4 more
core   +5 more sources

Inducible auto-phosphorylation regulates a widespread family of nucleotidyltransferase toxins [PDF]

Nature Communications
Nucleotidyltransferases (NTases) control diverse physiological processes, including RNA modification, DNA replication and repair, and antibiotic resistance.
Tom J. Arrowsmith, Xibing Xu, Shangze Xu, Ben Usher, Peter Stokes, Megan Guest, Agnieszka K. Bronowska, Pierre Genevaux, Tim R. Blower   +8 more
doaj   +4 more sources

Expanding the Available RNA Labeling Toolbox With CutA Nucleotidyltransferase for Efficient Transcript Labeling with Purine and Pyrimidine Nucleotide Analogs

ChemBioChem
AbstractRNA labeling is an invaluable tool for investigation of the function and localization of nucleic acids. Labels are commonly incorporated into 3′ end of RNA and the primary enzyme used for this purpose is RNA poly(A) polymerase (PAP), which belongs to the class of terminal nucleotidyltransferases (NTases).
Rafal Tomecki, Karolina Drazkowska, Rafal Madaj, Adam Mamot, Stanislaw Dunin‐Horkawicz, Pawel J. Sikorski   +5 more
core   +6 more sources

A eubacterial origin for the human tRNA nucleotidyltransferase? [PDF]

, 2001
tRNA CCA-termini are generated and maintained by tRNA nucleotidyltransferases. Together with poly(A) polymerases and other enzymes they belong to the nucleotidyltransferase superfamily.
Mörl, M. ; https://orcid.org/, Mörl, Mario, Reichert, Andreas S., Thurlow, D., A.S. Reichert, M. Mörl, Reichert, A., Thurlow, D. L., Reichert, A. S., Mörl, M., D.L. Thurlow   +10 more
core   +1 more source

RNA-specific ribonucleotidyl transferases [PDF]

, 2007
RNA-specific nucleotidyl transferases (rNTrs) are a diverse family of template-independent polymerases that add ribonucleotides to the 3'-ends of RNA molecules.
Keller, W., Martin, G., W. Keller, G. Martin   +3 more
core   +1 more source

Nontemplated Terminal Nucleotidyltransferase Activity of Double-Stranded RNA Bacteriophage φ6 RNA-Dependent RNA Polymerase [PDF]

Journal of Virology, 2008
ABSTRACT The replication and transcription of double-stranded RNA (dsRNA) viruses occur within a polymerase complex particle in which the viral genome is enclosed throughout the entire life cycle of the virus. A single protein subunit in the polymerase complex is responsible for the template-dependent RNA polymerization activity. The isolated
Minna M, Poranen, Minni R L, Koivunen, Dennis H, Bamford   +2 more
openaire   +2 more sources

A new yeast poly(A) polymerase complex involved in RNA quality control [PDF]

, 2005
Eukaryotic cells contain several unconventional poly(A) polymerases in addition to the canonical enzymes responsible for the synthesis of poly(A) tails of nuclear messenger RNA precursors.
Sabine Dettwiler (27038), Arno Friedlein (27039), Keller Walter, Stepánka Vanácová, Vanacova, S., Blank Diana, Martin Georges, Gérard Keith, Keller, W., Sabine Dettwiler, Jeannette Wolf (27035), Langen, H., Keith, G., Diana Blank (27037), Vanˇácˇová Sˇteˇpánka, Vanácová Stepánka, Georges Martin, Hanno Langen, Diana Blank, Hanno Langen (27040), Gérard Keith (27041), Keith Gérard, Walter Keller (27042), Wolf, J., Jeannette Wolf, Blank, D., Arno Friedlein, Wolf Jeannette, Štěpánka Vaňáčová (27034), Walter Keller, Friedlein, A., Langen Hanno, Friedlein Arno, Dettwiler, S., Dettwiler Sabine, Georges Martin (27036), Martin, G.   +36 more
core   +1 more source

Development of methods for the analysis of deep sequencing data; applications to the discovery of functions of RNA-binding proteins [PDF]

, 2014
With the recent advances in nucleotide sequencing technologies, it became easy to generate tens of millions of reads with genome- or transcriptome-wide distribution with reduced cost and high accuracy.
Bilen, Biter
core   +1 more source

Two cGAS-like receptors induce antiviral immunity in Drosophila [PDF]

, 2021
In mammals, cyclic GMP–AMP (cGAMP) synthase (cGAS) produces the cyclic dinucleotide 2′3′-cGAMP in response to cytosolic DNA and this triggers an antiviral immune response.
Frederiksen, N. (Ninna Ahlmann), Winther, K. (Kasper), Hartmann, R. (Rune), Grønbjerg Winther, Kasper, Ai, Xianlong, Cai, Hua, Andersen, L. (Line Lykke), Chen, Y. (Yuqiang), Kleigrewe, Karin, Simonsen, B. (Bine), Liu, Jiyong, Li, L. (Lihua), Pichlmair, Andreas, Dalskov, L. (Louise), Cai, H. (Hua), Wei, Ziming, Hartmann, Rune, Andersen, Line Lykke, Wei, Z. (Ziming), Simonsen, Bine, Liu, J. (Jiyong), Chen, Yuqiang, Frederiksen, Ninna Ahlmann, Imler, Jean-Luc, Holleufer, Andreas, Kleigrewe, K. (Karin), Holleufer, A. (Andreas), Pichlmair, A. (Andreas), Imler, J. (Jean-Luc), Ai, X. (Xianlong), Dalskov, Louise, Gad, Hans Henrik, Deng, H. (Huimin), Gad, H. (Hans Henrik), Li, Lihua, Deng, Huimin   +35 more
core   +1 more source