Structures and mechanism of E2‐CBASS anti‐phage system [PDF]
mLifeBacteria deploy diverse innate immune systems to combat bacteriophage infections. The cyclic‐oligonucleotide‐based anti‐phage signaling system (CBASS) is a type of innate prokaryotic immune system.
Jun Xiao +7 more
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Mechanism of Mg2+-Accompanied Product Release in Sugar Nucleotidyltransferases [PDF]
Structure, 2018 The nucleotidyl transfer reaction, catalyzed by sugar nucleotidyltransferases (SNTs), is assisted by two active site Mg 2+ ions. While studying this reaction using X-ray crystallography, we captured snapshots of the pyrophosphate (product) as it exits ...
Neha Vithani +2 more
exaly +7 more sources
Bicuculline reversal of aminoglycoside O-nucleotidyltransferase EanT-1–mediated kanamycin resistance [PDF]
BMC MicrobiologyBackground Kanamycin, an aminoglycoside, is an effective broad-spectrum antimicrobial agent against bacterial infections. However, the clinical efficacy of aminoglycosides has been overshadowed by the emergence of resistance mechanisms involving enzyme ...
Jiahui Wang +8 more
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Nature Communications, 2023 Purine-containing nucleotide second messengers regulate diverse cellular activities. Cyclic di-pyrimidines mediate anti-phage functions in bacteria; however, the synthesis mechanism remains elusive.
Tzu-Ping Ko +2 more
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RNA Tailing by Nucleotidyltransferases in Plants: Mechanisms, Functions, and Biological Significance [PDF]
PlantsRNA tailing, the non-templated addition of nucleotides to RNA 3′ ends, is a conserved post-transcriptional modification that plays a critical role in regulating RNA metabolism.
Xintong Xu +6 more
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A eubacterial origin for the human tRNA nucleotidyltransferase? [PDF]
Biological Chemistry, 2001 tRNA CCA-termini are generated and maintained by tRNA nucleotidyltransferases. Together with poly(A) polymerases and other enzymes they belong to the nucleotidyltransferase superfamily.
Aebi M. +12 more
core +6 more sources
Two complementing in vivo selection systems based on CCA-trimming exonucleases as a tool to monitor, select and evaluate enzymatic features of tRNA nucleotidyltransferases [PDF]
RNA BiologytRNA nucleotidyltransferase represents a ubiquitous and essential activity that adds the indispensable CCA triplet to the 3’-end of tRNAs. To fulfill this function, the enzyme contains a set of highly conserved motifs whose coordinated interplay is ...
Karolin Wellner +5 more
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Thermodynamics of binding of divalent magnesium and manganese to uridine phosphates: implications for diabetes-related hypomagnesaemia and carbohydrate biocatalysis [PDF]
Chemistry Central Journal, 2008 Background Although the necessity of divalent magnesium and manganese for full activity of sugar nucleotidyltransferases and glycosyltransferases is well known, the role of these metal cations in binding the substrates (uridine 5'-triphosphate, glucose-1-
Pohl Nicola L +2 more
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Identification and Characterization of Mammalian Mitochondrial tRNA nucleotidyltransferases [PDF]
Journal of Biological Chemistry, 2001 The CCA-adding enzyme (ATP:tRNA adenylyltransferase or CTP:tRNA cytidylyltransferase (EC )) generates the conserved CCA sequence responsible for the attachment of amino acid at the 3' terminus of tRNA molecules. It was shown that enzymes from various organisms strictly recognize the elbow region of tRNA formed by the conserved D- and T-loops.
Tsutomu Suzuki +2 more
exaly +3 more sources
ant(6)-I Genes Encoding Aminoglycoside O-Nucleotidyltransferases Are Widely Spread Among Streptomycin Resistant Strains of Campylobacter jejuni and Campylobacter coli [PDF]
Frontiers in Microbiology, 2018 Thermotolerant Campylobacter species C. jejuni and C. coli are actually recognized as the major bacterial agent responsible for food-transmitted gastroenteritis. The most effective antimicrobials against Campylobacter are macrolides and some, but not all
Lorena Hormeño +9 more
doaj +5 more sources