Results 21 to 30 of about 6,924 (153)
Frontiers in Microbiology, 2016 Here we report the complete genome sequence of the chemoorganotrophic, extremely thermophilic bacterium, Dictyoglomus turgidum, which is a Gram negative, strictly anaerobic bacterium. D. turgidum and D.
Phillip Brumm +6 more
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Molecular mechanisms of template-independent RNA polymerization by tRNA nucleotidyltransferases
Frontiers in Genetics, 2014 The universal 3'-terminal CCA sequence of tRNA is built and/or synthesized by the CCA-adding enzyme, CTP:(ATP) tRNA nucleotidyltransferase. This RNA polymerase has no nucleic acid template, but faithfully synthesizes the defined CCA sequence on the 3 ...
Kozo eTomita, Seisueke eYamashita
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A Eubacterial Origin for the Human tRNA Nucleotidyltransferase? [PDF]
Biological Chemistry, 2001 tRNA CCA-termini are generated and maintained by tRNA nucleotidyltransferases. Together with poly(A) polymerases and other enzymes they belong to the nucleotidyltransferase superfamily. However, sequence alignments within this family do not allow to distinguish between CCA-adding enzymes and poly(A) polymerases. Furthermore, due to the lack of sequence
Reichert, A. S. +2 more
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Purification and Characterization of a Mutant tRNA Nucleotidyltransferase [PDF]
European Journal of Biochemistry, 1980 tRNA nucleotidyltransferase has been extensively purified from a mutant strain of Escherichia coli which displays greatly decreased AMP incorporation, but normal CMP incorporation. The defect in AMP incorporation is retained throughout the purification of the mutant protein. The mutant protein behaves identically to the wild‐type protein with regard to
R G, McGann, M P, Deutscher
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The influence of spermidine on the reaction of RNA nucleotidyltransferase
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1968 Abstract 1. 1. RNA synthesis, catalyzed by RNA nucleotidyltransferase ( Escherichia coli B) and directed by native, double-stranded DNA, is significantly increased by spermidine. 2. 2. By use of doubly labeled ATP ([8- 14 C]- and [γ- 32 P]-) and by zone centrifugation, we could show that, in the presence of spermidine, more RNA chains are ...
Petersen, E. E., Kroeger, H., Hagen, U.
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mBio, 2015 The aminoglycosides are highly effective broad-spectrum antimicrobial agents. However, their efficacy is diminished due to enzyme-mediated covalent modification, which reduces affinity of the drug for the target ribosome.
Georgina Cox +3 more
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Frontiers in ParasitologyRNA-specific nucleotidyltransferases (rNTrs) add nontemplated nucleotides to the 3′ end of RNA. Two noncanonical rNTRs that are thought to be poly(A) polymerases (PAPs) have been identified in the mitochondria of trypanosomes – KPAP1 and KPAP2.
Clara M. Smoniewski +6 more
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Enzymes are enriched in bacterial essential genes.
PLoS ONE, 2011 Essential genes, those indispensable for the survival of an organism, play a key role in the emerging field, synthetic biology. Characterization of functions encoded by essential genes not only has important practical implications, such as in identifying
Feng Gao, Randy Ren Zhang
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The inhibition of deoxyribonucleic acid nucleotidyltransferase by stilboestrol derivatives [PDF]
Biochemical Journal, 1968 The inhibition by diethylstilboestrol of DNA nucleotidyltransferase isolated from calf thymus was studied. The inhibition exercised by diethylstilboestrol appears to obey competitive kinetics with respect to DNA primer. The activities of both replicative and terminal enzymes were affected to the same extent. There was no evidence of binding between DNA
A R, Fahmy, K, Griffiths
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PLoS Genetics, 2017 The emergence and spread of antibiotic resistance among Acinetobacter spp. have been investigated extensively. Most studies focused on the multiple antibiotic resistance genes located on plasmids or genomic resistance islands.
Gang Zhang +7 more
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