Results 11 to 20 of about 1,628 (157)

The Ti Plasmid-Encoded VirJ Functions as a Lysyl-Phosphatidylglycerol Hydrolase in Agrobacterium tumefaciens. [PDF]

Mol Microbiol
We identify the long‐known virulence factor VirJ as a lysyl‐phosphatidylglycerol (L‐PG) hydrolase. Like the chromosomally encoded virulence factor AcvB, it prevents excessive accumulation of L‐PG in the membrane, thereby maintaining efficient T‐DNA transfer via the Type IV secretion system (T4SS) in Agrobacterium tumefaciens.
Lotz B, Brodskaia L, Fritz C, Hebecker S, Tauchert MK, Breisch J, Jahn D, Narberhaus F, Moser J, Aktas M.   +9 more
europepmc   +2 more sources

Application of a GRF-GIF chimera enhances plant regeneration for genome editing in tomato. [PDF]

Plant Biotechnol J
Summary Genome editing has become a routine tool for functionally characterizing plant and animal genomes. However, stable genome editing in plants remains limited by the time‐ and labour‐intensive process of generating transgenic plants, as well as by the efficient isolation of desired heritable edits.
Swinnen G, Lizé E, Loera Sánchez M, Stolz S, Soyk S.   +4 more
europepmc   +3 more sources

A Fluorescence-Based Transient Expression Assay for the Analysis of Upstream Open Reading Frames in Plants. [PDF]

Plant Direct
ABSTRACT Upstream open reading frames (uORFs) are regulatory elements present in the 5′ leaders of mRNA that can significantly impact downstream gene expression in eukaryotes. In crop engineering, editing of uORFs can provide an avenue to upregulate expression of native genes without the need to add persistent transgenic copies.
Haas BE, Saif F, Bolger E, Doran L, Rosales E, Kim A, Burgess SJ, Long SP.   +7 more
europepmc   +2 more sources

A Multi-Organ Atlas Links Gut Microbial Metabolites to Systemic Redox Changes in Aging Mice. [PDF]

Aging Cell
A gut‐derived metabolic signature drives systemic aging phenotypes. Integrated multi‐omics profiling of young versus aged mice identifies a conserved aging signature characterized by the depletion of protective lysophosphatidylcholines (LPC 22:0) and the concurrent accumulation of pro‐oxidative microbial catabolites (TMAO, IAA).
Sajid S, Huang J, Kong S, Lai C, Tan Z, Shao Y, Guo L.   +6 more
europepmc   +2 more sources

Negative control of octopine degradation and transfer genes of octopine Ti plasmids in Agrobacterium tumefaciens [PDF]

Journal of Bacteriology, 1979
The regulatory system that controls the expression of the Ti plasmid-borne octopine degradation (uad) and transfer (tra) genes in Agrobacterium tumefaciens was studied. A deletion mutant derived from the cointegrate plasmid R702::Ti-B6S3 was isolated, which was compatible with a wild-type Ti plasmid and which had retained the uad genes.
P M, Klapwijk, R A, Schilperoort
openaire   +2 more sources

The Presence and Characterization of a virF Gene on Agrobacterium vitis Ti Plasmids

Molecular Plant-Microbe Interactions, 1998
Octopine and nopaline strains of Agrobacterium tumefaciens differ in their ability to induce tumors on Nicotiana glauca. The presence of a virF locus on the octopine Ti plasmid makes N.
B. Schrammeijer, J. Hemelaar, P. J. J. Hooykaas   +2 more
doaj   +1 more source

Capsella rubella TGA4, a bZIP transcription factor, causes delayed flowering in Arabidopsis thaliana [PDF]

Archives of Biological Sciences, 2016
Flowering time is usually regulated by many environmental factors and endogenous signals. TGA family members are bZIP transcription factors that bind to the octopine synthase element, which has been closely linked to defense/stress responses ...
Li Maofu, Wang Hua, Yang Yuan, Jin Wanmei   +3 more
doaj   +1 more source

Utilization of Octopine and Nopaline by Agrobacterium [PDF]

Journal of Bacteriology, 1973
Tests for utilization of d -octopine and nopaline in defined media containing a carbon and nitrogen source were made on 60 strains of Agrobacterium representing four species and on a representative of each of five species of Rhizobium . Among 46 virulent strains of
J A, Lippincott, R, Beiderbeck, B B, Lippincott   +2 more
openaire   +2 more sources

Insights into the mechanism of ligand binding to octopine dehydrogenase from Pecten maximus by NMR and crystallography.

PLoS ONE, 2010
Octopine dehydrogenase (OcDH) from the adductor muscle of the great scallop, Pecten maximus, catalyzes the NADH dependent, reductive condensation of L-arginine and pyruvate to octopine, NAD(+), and water during escape swimming and/or subsequent recovery.
Sander H J Smits, Tatu Meyer, Andre Mueller, Nadine van Os, Matthias Stoldt, Dieter Willbold, Lutz Schmitt, Manfred K Grieshaber   +7 more
doaj   +1 more source

Screening of phytocompounds, molecular docking studies, and in vivo anti-inflammatory activity of heartwood aqueous extract of Pterocarpus santalinus L.f.

Asian Pacific Journal of Tropical Biomedicine, 2021
Objective: To evaluate the anti-inflammatory potential of aqueous extract of Pterocarpus santalinus L.f. heartwood using molecular docking and in vivo experiment.
C N Shanti Vasudevan, Bibu John Kariyil, D Athira Nair, I'ma Neerakkal   +3 more
doaj   +1 more source