Results 51 to 60 of about 9,432,011 (294)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

The Ocular Surface Microbiome in Homeostasis and Dysbiosis

Microorganisms
The ocular surface microbiome consists of microorganisms that play an important role in maintaining homeostasis and preventing disease from invading pathogens.
Fiza Tariq, Navpreet K. Hehar, DeGaulle I. Chigbu   +2 more
doaj   +1 more source

UiO‐66 metal–organic frameworks in biomedicine: From structural tunability to bioimaging, photodiagnostics, and photodynamic cancer therapy

FEBS Open Bio, EarlyView.
UiO‐66(Zr) metal–organic frameworks are chemically stable, biocompatible, and highly tunable nanomaterials. Their modular structure enables controlled drug delivery, multimodal bioimaging, and light‐activated photodynamic therapy, supporting integrated diagnostic and therapeutic (theranostic) applications in cancer and biomedical research.
Veronika Huntošová, Grigorii Rakhalskii, Miroslav Almáši   +2 more
wiley   +1 more source

Impact of Exposomes on Ocular Surface Diseases

International Journal of Molecular Sciences, 2023
Ocular surface diseases (OSDs) are significant causes of ocular morbidity, and are often associated with chronic inflammation, redness, irritation, discomfort, and pain. In severe OSDs, loss of vision can result from ocular surface failure, characterised by limbal stem cell deficiencies, corneal vascularisation, corneal opacification, and surface ...
Merrelynn Hong, Louis Tong, Jodhbir S. Mehta, Hon Shing Ong   +3 more
openaire   +2 more sources

Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes

FEBS Open Bio, EarlyView.
Group A Streptococcus (GAS) acquires covRS mutations driving a hypervirulent bacterial state, frequently associated with invasive disease‐like necrotizing fasciitis. We demonstrate that the newly emerged M1UK GAS lineage can also acquire these mutations.
Jarrad Pritchard, Emma‐Jayne Proctor, Jiawa Wang, Nikolas P. Johnston, Andrew Hayes, Georgia McCorkell, Haibo Yu, Jai Tree, Mark R. Davies, Mark J. Walker, Ronald Sluyter, Stephan Brouwer, Martina L. Sanderson‐Smith   +12 more
wiley   +1 more source

Autologous serum for ocular surface diseases [PDF]

Arquivos Brasileiros de Oftalmologia, 2008
Autologous serum has been used to treat dry eye syndrome for many years. It contains several growth factors, vitamins, fibronectin and other components that have been considered important for corneal and conjunctival integrity. Serum eye drops are usually prepared as an unpreserved blood solution.
Quinto, Guilherme Goulart, Campos, Mauro, Behrens, Ashley   +2 more
openaire   +4 more sources

Ocular Surface Diseases in Patients With Diabetes

Cureus, 2022
Purpose Diabetes is a major cause of ocular morbidity as multiple mechanisms play a role in inducing inflammatory changes in the eye. Diabetic retinopathy is the most common complication and is well-documented. However, in the era of modern medicine, attention is also being focused on ocular surface changes in diabetes.
Naik, Kunj, Magdum, Renu, Ahuja, Amod, Kaul, Sucheta, S, Johnson, Mishra, Ashish, Patil, Mayur, Dhore, Dr. Nilay, Alapati, Aparna   +8 more
openaire   +2 more sources

Review and analysis of grading scales for ocular surface staining

, 2019
Vital dye staining has been used for over a century to assess the severity of ocular surface disease. However, despite common usage, a universally accepted “gold standard” grading scale does not exist for corneal and conjunctival staining, which can ...
Simpson, T, Begley, Carolyn G, Caffery, B, Chalmers, R, Situ, Ping, Nelson, J D   +5 more
core   +1 more source

Ocular Surface Disease Index and Ocular Thermography in Keratoconus Patients

Journal of Ophthalmology, 2020
Purpose. Keratoconus (KC) has been defined as a “noninflammatory” corneal disease, but recent studies have noted a potential inflammatory origin. We analysed the Ocular Surface Disease Index (OSDI) and ocular surface temperature (OST) in KC patients ...
Orsolya Németh, Achim Langenbucher, Timo Eppig, Sabine Lepper, Georgia Milioti, Aladin Abdin, Zoltán Zsolt Nagy, Berthold Seitz, Nóra Szentmáry   +8 more
doaj   +1 more source

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

FEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas, Aaron D. Morgan, Ignas Čiurlionis, Christian Böhm, Stefan Jockenhövel, Willi Jahnen‐Dechent, Marc A. M. J. van Zandvoort   +6 more
wiley   +1 more source