Results 171 to 180 of about 11,991 (190)

One-lane sequence analysis of oligodeoxyribonucleotides

Analytical Biochemistry, 1986
Treatment of 5'-end 32P-labeled oligodeoxyribonucleotides with 0.4 M aqueous piperidinium formate, pH 2, at 37 degrees C for 6 h, followed by treatment with 1 M aqueous piperidine at 90 degrees C for 6 h, produces, after electrophoresis through 27% polyacrylamide sequencing gels, one-dimensional distributions of radioactivity from which the base ...
B J, Ambrose, M M, Castro, R C, Pless
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Oligodeoxyribonucleotides: Chemical Synthesis in Anhydrous Base

Science, 1970
The sugar hydroxyl of a methoxytrityldeoxyribonucleoside reacts with a methoxytrityldeoxyribonucleoside phosphorofluoridate in dimethylformamide and potassium tertiary butoxide to yield the protected dinucleoside monophosphate. The reaction is fast and specific, and is used in a stepwise synthesis to prepare trinucleoside diphosphate and ...
R, Von Tigerstrom, M, Smith
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Phosphite Synthesis of Oligodeoxyribonucleotides

Russian Chemical Reviews, 1988
Data in the literature published between 1983 and 1986 on the synthesis of oligodeoxyribonucleotides from nucleoside derivatives of phosphorous acid are reviewed. Attention is mainly focused on the techniques employed for preparing initial monomers for condensation.
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Selective modification of cytosines in oligodeoxyribonucleotides

Bioconjugate Chemistry, 1992
A single deoxycytidine residing in an oligodeoxyribonucleotide which also contains 5-methyldeoxycytidines can be selectively derivatized with various alkylamines by sodium bisulfite-catalyzed transamination. Selective transamination results because 5-methylcytosine, unlike cytosine, does not form a bisulfite adduct.
P S, Miller, C D, Cushman
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Enantio- and meso-oligodeoxyribonucleotides.

Nucleic acids symposium series, 1992
As sugar-modified oligodeoxyribonucleotides, L-homooligomers (L-dA12 and L-dT12) and a L/D-alternative oligomer ((L-dA-D-dA)6) were synthesized. These oligomers were quite resistant towards phosphodiesterase degradations. L-dA12 formed complexes with poly(dT) and poly(U) at 0 degree C with different stabilities, on the other hand L-dT12 did not show ...
S, Fujimori, N, Iwanami, K, Shudo, Y, Hashimoto   +3 more
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Synthesis of cyclic branched oligodeoxyribonucleotides

1991
The synthesis of two cyclic branched oligonucleotides, a trimer and a tetramer of thymidylic acid, has been successfully achieved exploiting a phosphotriester-based synthetic protocol.
DE NAPOLI, LORENZO, MONTESARCHIO, DANIELA, PICCIALLI, GENNARO, C. Santacroce, A. Messere   +4 more
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Chemical Synthesis of Oligodeoxyribonucleotides

1987
Oligodeoxyribonucleotides of defined sequence may now be synthesised as a matter of routine at a reasonable cost in both time and materials. As both applications of and synthetic methods for oligodeoxyribonucleotides are much more highly developed than those for oligoribonucleotides, the term ‘oligonucleotide’ has come to be synonymous with ...
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Atmospheric Pressure Photoionization Mass Spectrometry of Oligodeoxyribonucleotides

European Journal of Mass Spectrometry, 2008
Small oligonucleotides (di- and trimers) were investigated by atmospheric pressure photoionization (APPI) with focus on the fragmentation mechanisms. The fragmentation patterns of these biomolecular ions have been monitored under dopant-assisted photoionization (DA-APPI) conditions.
Bagag, A., Giuliani, A., Laprévote, O.
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Oligodeoxyribonucleotides as Site-Specific Mutagens

1981
①Classical genetic analysis of cells and viruses involves spontaneous or induced mutation together with screening or selection for a changed phenotype followed by assignment of the changed DNA to a specific location in the genome. This approach has been very useful in defining the various functions of DNA both in coding and regulatory regions ②The ...
Michael Smith, Shirley Gillam
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The automated synthesis of oligodeoxyribonucleotides

Trends in Biotechnology, 1985
Abstract Progress in science is a history of the synergistic relationship between technology and theory: growth in theoretical understanding requires and initiates technological change; technological advance opens up new areas of knowledge. The history of molecular genetics is but a single illustration of this process.
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