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Self-Association of Telomeric Short Oligodeoxyribonucleotides Containing a dG Cluster.
, 1991 Toshio Shida +3 more
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An efficient method for the sequence analysis of oligodeoxyribonucleotides
Analytical Biochemistry, 1983Modifications of the chemical method of DNA sequence analysis that permit rapid and reliable sequence determination of single-stranded oligodeoxyribonucleotides as short as 4 nucleotides in length are reported. The principal changes made were increasing the level of chemical modification and optimizing the conditions for recovery of the chemically ...
Marek Michalak, Bernard R Glick
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Improved synthesis of oligodeoxyribonucleotides
Tetrahedron Letters, 1990An improved synthesis of oligodeoxyribonucleotides was achieved with NPE- and NPEOC-protected phosphoramidites leading to easier and time-saving purification and isolation of the gene fragments.
K P, Stengele, W, Pfleiderer
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A Chemical 5′-Phosphorylation of Oligodeoxyribonucleotides
DNA, 1986To circumvent the use of T4 polynucleotide kinase and ATP for the 5'-phosphorylation of synthetic oligodeoxyribonucleotides after deprotection and purification, we have developed a new chemical phosphorylation reagent that can be used on automated DNA synthesis instruments.
T, Horn, M S, Urdea
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Selective modification of cytosines in oligodeoxyribonucleotides
Bioconjugate Chemistry, 1992A single deoxycytidine residing in an oligodeoxyribonucleotide which also contains 5-methyldeoxycytidines can be selectively derivatized with various alkylamines by sodium bisulfite-catalyzed transamination. Selective transamination results because 5-methylcytosine, unlike cytosine, does not form a bisulfite adduct.
P S, Miller, C D, Cushman
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Versatile synthesis of oligodeoxyribonucleotide–oligospermine conjugates
Nature Protocols, 2007A protocol for the rapid, automated synthesis of oligospermine-oligonucleotide sequences is described. To this end, a protected spermine phosphoramidite derivative was synthesized in six steps from spermine and used as the fifth synthon in an oligonucleotide synthesizer. Parameters were optimized to reach greater than 95% coupling yields.
Voirin, E., Kotera, M., Behr, J.P.
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PHOSPHONOACETATE DERIVATIVES OF OLIGODEOXYRIBONUCLEOTIDES
Nucleosides and Nucleotides, 1996Abstract A deoxyribodinucleotide phosphonoacetate derivative has been prepared, separated into individual diastereomers, and incorporated into oligodeoxyribonucleotides possessing alternating phosphodiester and phosphonoacetate backbone linkages. The hybridization properties and enzymatic stabilities of these oligonucleotides have been studied.
M Jonathan Rudolph +3 more
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Red light activated phosphothioate oligodeoxyribonucleotides
Collection Symposium Series, 2008Hairpin-structured phosphorothioate oligodeoxyribonucleotides containing a singlet oxygen-sensitive linker in the loop were prepared. These compounds do not bind complementary nucleic acids in the dark. Upon irradiation with red light in the presence of chlorine e6 the linker within these compounds is cleaved and a single-stranded ...
Alexandru, Rotaru +2 more
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