Results 81 to 90 of about 732,131 (343)

The cooperative regulation of miR‐221 by APE1 and AUF1 impacts p27Kip1 defining a miR signature relevant for cervical cancer

FEBS Open Bio, EarlyView.
A regulatory axis involving APE1, AUF1, and miR‐221 is proposed. Pri‐miR‐221 is processed by DROSHA and DICER to generate mature miR‐221, which targets p27Kip1 mRNA. APE1 and AUF1 compete for pre‐miR‐221 binding. Reduced APE1/AUF1 levels impair miR‐221 biogenesis, decrease p27Kip1 mRNA degradation, and promote cell cycle progression, chemoresistance ...
Matilde Clarissa Malfatti, Nicolò Gualandi, Gianluca Tell, Giulia Antoniali   +3 more
wiley   +1 more source

cDNA Cloning Demonstrates the Expression of Pregnancy-Specific Glycoprotein Genes, a Subgroup of the Carcinoembryonic Antigen Gene Family, in Fetal Liver [PDF]

, 1989
The pregnancy-specific glycoprotein (PSG) genes constitute a subgroup of the carcinoembryonic antigen (CEA) gene family. Here we report the cloning of four cDNAs coding for different members of the PSG family from a human fetal liver cDNA library.
Thompson, John A., Weiss, Martina, Zimmermann, Wolfgang   +2 more
core   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Extinction of tyrosine aminotransferase gene activity in somatic cell hybrids involves modification and loss of several essential transcription factors [PDF]

, 1993
Extinction is defined as the loss of cell type-specific gene expression that occurs in somatic cell hybrids derived by fusion of cells with dissimilar phenotypes.
Boshart, Michael, Nitsch, Doris, Schütz, Günther   +2 more
core   +1 more source

MiR‐513a promotes human erythroid differentiation by modulating c‐Jun

FEBS Open Bio, EarlyView.
During early human erythropoiesis, miR‐513a promoted erythroid differentiation in primary human CD34+ hematopoietic stem‐progenitor cells and human TF‐1 erythroleukemic cells by indirectly decreasing c‐Jun and phospho‐c‐Jun expression, which are associated with increased GATA1 expression.
MinJung Kim, Brittany Taylor, Shannon Bolten, Christian L. Eberly, Mahliya Abdurahman, T. Michael Creed, Acong Yang, Taylor L. Hatchet, Tristan Dyson, Shuo Gu, Curt I. Civin, Tami J. Kingsbury   +11 more
wiley   +1 more source

Antisense oligonucleotide therapy for spinocerebellar ataxia type 2

Nature, 2017
There are no disease-modifying treatments for adult human neurodegenerative diseases. Here we test RNA-targeted therapies in two mouse models of spinocerebellar ataxia type 2 (SCA2), an autosomal dominant polyglutamine disease.
Daniel R. Scoles, P. Meera, Matthew Schneider, S. Paul, W. Dansithong, K. Figueroa, G. Hung, F. Rigo, C. F. Bennett, T. S. Otis, Stefan M. Pulst   +10 more
semanticscholar   +1 more source

Digital twins to accelerate target identification and drug development for immune‐mediated disorders

FEBS Open Bio, EarlyView.
Digital twins integrate patient‐derived molecular and clinical data into personalised computational models that simulate disease mechanisms. They enable rapid identification and validation of therapeutic targets, prediction of drug responses, and prioritisation of candidate interventions.
Anna Niarakis, Philippe Moingeon
wiley   +1 more source

Two new rapid SNP-typing methods for classifying Mycobacterium tuberculosis complex into the main phylogenetic lineages [PDF]

, 2012
There is increasing evidence that strain variation in Mycobacterium tuberculosis complex (MTBC) might influence the outcome of tuberculosis infection and disease.
A Deshpande, AC Schürch, AC Schürch, AG Tsolaki, BC de Jong, BC de Jong, Bijaya Malla, C Allix-Béguec, C Bouakaze, C Bouakaze, C Demay, CM Sassetti, CM Sassetti, CRE McEvoy, D Portevin, D Yeboah-Manu, DA Milner Jr, David Stucki, Dorothy Yeboah-Manu, E Abadia, ES Click, G Thwaites, H Yesilkaya, I Comas, I Comas, I Filliol, I Filliol, I Parwati, Igor Mokrousov, IL Bergval, Iñaki Comas, J Kamerbeek, J Song, J Zhi, J Zhi, JD van Embden, JP Schouten, Julia Feldmann, K Okonechnikov, K Tamura, L Baker, L Fenner, L Fenner, L Flores, L Kaderali, L Wang, LE Espinosa de los Monteros, LS Cowan, Lukas Fenner, M Ben Shabat, M Caws, M Caws, M Coscolla, M Kato-Maeda, M Kato-Maeda, Mireia Coscollà, MM Gutacker, MP Nicol, NH Smith, O Mestre, P Keim, P Supply, P Supply, PM Groenen, PM Holland, PM Vallone, R Hershberg, S Gagneux, S Homolka, S Kim, S Sreevatsan, S-H Lee, SA Dunbar, SD Bentley, Sebastien Gagneux, Simon Hostettler, Sonia Borrell, ST Cole, T Pearson, TA Halse, Thembela Huna, U Nübel, WP Maddison   +82 more
core   +4 more sources

Identifying transcription factors controlling the basal expression of human MRP4 highlights a substantial role for Sp1

FEBS Open Bio, EarlyView.
The MRP4 transporter exports several drugs and signaling molecules. Here, we identified key promoter elements regulating basal MRP4 expression. Using reporter assays, we defined a conserved region with essential Sp1 and contributory Ets sites, which controlled basal MRP4 expression.
Debora Singer, Angelique Kragl, Katrin Ziems, Juliane Glaubitz, Sophie Grammbauer, Susanne Hildebrandt, Mladen V. Tzvetkov, Gabriele Jedlitschky   +7 more
wiley   +1 more source

2′-O-Methyl at 20-mer Guide Strand 3′ Termini May Negatively Affect Target Silencing Activity of Fully Chemically Modified siRNA

Molecular Therapy: Nucleic Acids, 2020
Small interfering RNAs (siRNAs) have the potential to treat a broad range of diseases. siRNAs need to be extensively chemically modified to improve their bioavailability, safety, and stability in vivo.
Sarah M. Davis, Jacquelyn Sousa, Lorenc Vangjeli, Matthew R. Hassler, Dimas Echeverria, Emily Knox, Anton A. Turanov, Julia F. Alterman, Anastasia Khvorova   +8 more
doaj   +1 more source