Results 131 to 140 of about 708,274 (274)

CRISPR applications for Duchenne muscular dystrophy: From animal models to potential therapies

WIREs Mechanisms of Disease, Volume 15, Issue 1, January/February 2023., 2023
CRISPR‐Cas9 gene‐editing technology enables the rapid generation of animal models for Duchenne muscular dystrophy research and has potential to be developed as CRISPR therapy for the long lasting genetic correction of causal mutations. Abstract CRISPR gene‐editing technology creates precise and permanent modifications to DNA.
Yu C. J. Chey, Jayshen Arudkumar, Annemieke Aartsma‐Rus, Fatwa Adikusuma, Paul Q. Thomas   +4 more
wiley   +1 more source

Comprehensive oligonucleotide array-comparative genomic hybridization analysis: new insights into the molecular pathology of the DMD gene

European Journal of Human Genetics, 2012
A. Ishmukhametova, P. Kien, D. Méchin, D. Thorel, M. Vincent, F. Rivier, C. Coubes, V. Humbertclaude, M. Claustres, S. Tuffery-Giraud   +9 more
semanticscholar   +1 more source

Discovery of miRNAs unique to actively transcribed erythroparvovirus infection in heart failure patients

ESC Heart Failure, EarlyView.
Abstract Aims miRNAs, small non‐coding RNAs, play key roles in gene regulation, cell differentiation and tissue development. They influence viral infection outcomes by directly interacting with viral genomes or modifying the host microenvironment. This study demonstrates miRNAs' ability to selectively suppress transcriptionally active erythroparvovirus,
Ganna Aleshcheva, Sara Salih, Christian Baumeier, Felicitas Escher, C. Thomas Bock, Heinz‐Peter Schultheiss   +5 more
wiley   +1 more source

A Comprehensive Review on Capillary Electrophoresis–Mass Spectrometry in Advancing Biomolecular Research

ELECTROPHORESIS, EarlyView.
ABSTRACT This review provides an in‐depth exploration of capillary electrophoresis–mass spectrometry (CE–MS) in biomolecular research from 2020 to 2024. CE–MS emerges as a versatile and powerful tool due to its numerous advantages, facilitating the analysis of various biomolecules, including proteins, peptides, oligonucleotides, and other metabolites ...
Laura Pont, María Vergara‐Barberán, Enrique Javier Carrasco‐Correa   +2 more
wiley   +1 more source

A Comprehensive Evaluation of Analytical Method Parameters Critical to the Reliable Assessment of Therapeutic mRNA Integrity by Capillary Gel Electrophoresis

ELECTROPHORESIS, EarlyView.
ABSTRACT In recent years, messenger ribonucleic acid (mRNA)‐lipid nanoparticle (LNP) biotherapeutics have demonstrated significant promise in disease treatment and prevention given their rapidly modifiable production processes and considerable capacity to adapt to complex or low‐yielding proteins of interest.
Jessica P. Tran, Jun Gao, Casey Lansdell, Barry Lorbetskie, Michael J. W. Johnston, Lisheng Wang, Xuguang Li, Huixin Lu   +7 more
wiley   +1 more source

Gene expression analysis identifies hypothetical genes that may be critical during the infection process of Xanthomonas citri subsp. citri

Electronic Journal of Biotechnology, 2019
Background: Gene expression analysis via microarray is widely used in phytobacteria to validate differential gene expression associated with virulence or to compare biological profiles of wild type and mutant strains. Here, we employed DNA microarrays to
Marcelo Luiz de Laia, Leandro Marcio Moreira, Janaína Fernandes Gonçalves, Maria Inês Tiraboschi Ferro, Any Caroliny Pinto Rodrigues, Jéssica Naiara dos Santos, Érica Barbosa Felestrino, Jesus Aparecido Ferro   +7 more
doaj  

Outcome of IWGT workshop on transcriptomic biomarkers for genotoxicity: Key considerations for bioinformatics

Environmental and Molecular Mutagenesis, EarlyView.
Abstract As a part of the International Workshop on Genotoxicity Testing (IWGT) in 2022, a workgroup was formed to evaluate the level of validation and regulatory acceptance of transcriptomic biomarkers that identify genotoxic substances. Several such biomarkers have been developed using various molecular techniques and computational approaches. Within
Matthew J. Meier, Florian Caiment, J. Christopher Corton, Roland Frötschl, Yurika Fujita, Danyel Jennen, Roman Mezencev, Carole Yauk   +7 more
wiley   +1 more source

Genetic epilepsies with myoclonic seizures: Mechanisms and syndromes

Epilepsia Open, EarlyView.
Abstract Genetic epilepsy with myoclonic seizures encompasses a heterogeneous spectrum of conditions, ranging from benign and self‐limiting forms to severe, progressive disorders. While their causes are diverse, a significant proportion stems from genetic abnormalities.
Antonietta Coppola, Marica Rubino, Antonella Riva, Pasquale Striano   +3 more
wiley   +1 more source

Organ‐on‐Chip: The Future of Nutrition Research in a One Health World

Food Frontiers, EarlyView.
Organ‐on‐chips offer a promising tool for evaluating food and feed safety. Organ‐on‐chips also present a scientifically robust and ethical alternative to animal studies. ABSTRACT The One Health approach emphasizes the interconnectedness of human, animal, and environmental health, recognizing that the health of each is interdependent and influenced by ...
Manuela Cassotta, Maria Elexpuru‐Zabaleta, Sandra Sumalla Cano, Yasmany Armas Diaz, Francesca Giampieri, Zou Xiaobo, Di Zhang, Giuseppe Grosso, Maurizio Battino   +8 more
wiley   +1 more source

The microbial community spatially varies during a Microcystis bloom event in Lake Kinneret

Freshwater Biology, Volume 68, Issue 2, Page 349-363, February 2023., 2023
Abstract Cyanobacterial blooms in lakes frequently display high spatial heterogeneity, which may drive temporal changes in microbial community structure. Here we report on the spatial (horizontal and vertical) structure of the cyanobacteria community and of the co‐occurring heterotroph microbial community during a major lake‐wide bloom event at Lake ...
Orna Schweitzer‐Natan, Maya Ofek‐Lalzar, Daniel Sher, Assaf Sukenik   +3 more
wiley   +1 more source