Results 131 to 140 of about 131,505 (250)

Microfluidic single‐cell technologies for immunotherapeutic target discovery: From design concepts to preclinical applications

VIEW, EarlyView.
Immunotherapy leverages the immune system to treat cancer and autoimmune diseases but is hindered by cellular heterogeneity in tissues and immune landscapes. Single‐cell technologies, particularly microfluidic‐enabled omics, revolutionize disease understanding by resolving this complexity at unprecedented resolution.
Haopu Wang, Kangfu Chen, Zongjie Wang
wiley   +1 more source

Conditional deletion of the multiple sclerosis susceptibility gene ATXN1 identifies cell‐autonomous effects in the B‐cell compartment

The FEBS Journal, EarlyView.
Ataxin‐1 is a polyglutamine protein associated with the etiology of spinocerebellar ataxia type 1 (SCA1) that has been recently found implicated in the risk of developing the autoimmune disorder multiple sclerosis (MS). Here, we took a conditional knockout approach to ablate ataxin‐1 exclusively in the B‐cell compartment and we found that this protein ...
Jonathan Jacob Carver, Rachael Riley Denbrock, Cindy Carolina Martines, Alessandro Didonna   +3 more
wiley   +1 more source

Plant organelle C‐to‐U RNA editing factors can operate successfully in yeast (Saccharomyces cerevisiae) as an easily amenable eukaryotic system for their functional analysis

The FEBS Journal, EarlyView.
Plant‐type pentatricopeptide repeat proteins capable of C‐to‐U RNA editing perform faithfully when expressed in a new heterologous system, the yeast Saccharomyces cerevisiae. They were tested with constitutive and inducible expression and with a set of different solubility tags. PPR56, PPR65, and PPR78 from P.
Shyam Ramanathan, Annika Hanraths, Jingchan Xie, Mareike Schallenberg‐Rüdinger, Volker Knoop   +4 more
wiley   +1 more source

EHD4 and ASAP2 are critical negative regulators of the claudin‐5‐based endothelial barrier

The FEBS Journal, EarlyView.
Cell‐surface CLDN‐5 protein levels can be evaluated using a probe that can bind to the extracellular domains of CLDN‐5. A probe derived from Clostridium perfringens enterotoxin allows us to isolate cells with high CLDN‐5 protein levels from a knockout cell library.
Yosuke Hashimoto, Gergő Porkoláb, Natalie Hudson, Jeffrey O'Callaghan, Nicole Hanley, Conor Delaney, Mária A. Deli, Peter Westenskow, Matthew Campbell   +8 more
wiley   +1 more source

Modulating a Massive Set of Biomolecular Structures by Sono-Mechanical Force. [PDF]

Adv Sci (Weinh)
Using low‐power ultrasound exposure, picoNewton forces are generated to release loaded drugs after unfolding secondary structures in DNA nanoassemblies. This opens a door to reversibly manipulate a large set of biomolecular structures both inside and outside cells without the damages incurred by high‐power sonications currently used in the field ...
Pokhrel P, Sharma G, Jenyk J, Lower A, Ji J, Shakya S, Haun J, Mao H.   +7 more
europepmc   +2 more sources

Phycocyanobilin biosynthesis in Galdieria sulphuraria requires isomerization of phycoerythrobilin synthesized by bilin reductases

The FEBS Journal, EarlyView.
The biosynthesis of bilins, tetrapyrroles essential for light harvesting and sensing, is performed by specific enzymes (FDBRs). In Galdieria sulphuraria, both phycobiliprotein types bind phycocyanobilin, despite lacking the canonical synthesizing gene PCYA. Instead, PEBA and PEBB are encoded, producing phycoerythrobilin, proposed to be later isomerized
Federica Frascogna, Nathan C. Rockwell, Jana Hartmann, Julie M. Mudler, Nicole Frankenberg‐Dinkel   +4 more
wiley   +1 more source

Molecular Testing in Sickle Cell Disease: From Newborn Screening to Transfusion Care

International Journal of Laboratory Hematology, EarlyView.
ABSTRACT Sickle cell disease (SCD) is one of the most frequent monogenic diseases worldwide and a highly heterogeneous and complex disease. SCD care carries several challenges. This includes early and accurate diagnosis as well as optimal red blood cell transfusion matching in this population carrying a high risk of alloimmunization.
Thomas Pincez, Yves D. Pastore
wiley   +1 more source

A signaling pathway mediating abscisic acid‐induced jasmonic acid biosynthesis during strawberry ripening

Journal of Integrative Plant Biology, EarlyView.
During strawberry ripening, abscisic acid signaling activates SnRK2.6, which phosphorylates and degrades the JAZ12 repressor. This releases the MYC2 transcription factor, directly activating jasmonic acid biosynthesis genes. The resulting jasmonic acid accumulation drives aroma production, thereby coupling ripening with scent formation through this ...
Kenan Jia, Baozhen Zeng, Zhengrong Dai, Xiaoming Zhang, Jing Wang, Chuanbao Wu, Chen Feng, Guohua Yan, Kaichun Zhang, Xuwei Duan, Wei Wang   +10 more
wiley   +1 more source

Gene therapy for epilepsy: An emerging, promising approach for a serious neurological disorder

Journal of Internal Medicine, EarlyView.
Abstract Gene therapy is emerging as a groundbreaking strategy for treating epilepsy, offering new hope to patients who do not respond to conventional medications. Despite advancements in anti‐seizure treatments, nearly 30%–40% of individuals with epilepsy continue to experience uncontrolled seizures, highlighting the urgent need for more effective and
Marco Ledri, Merab Kokaia
wiley   +1 more source

Fabrication of microcolumn arrays for high-throughput oligonucleotide synthesis using 3D printing. [PDF]

Sci Rep
Kim H, Kim J, Bang D.
europepmc   +1 more source