Results 151 to 160 of about 130,578 (251)

Conditional deletion of the multiple sclerosis susceptibility gene ATXN1 identifies cell‐autonomous effects in the B‐cell compartment

The FEBS Journal, EarlyView.
Ataxin‐1 is a polyglutamine protein associated with the etiology of spinocerebellar ataxia type 1 (SCA1) that has been recently found implicated in the risk of developing the autoimmune disorder multiple sclerosis (MS). Here, we took a conditional knockout approach to ablate ataxin‐1 exclusively in the B‐cell compartment and we found that this protein ...
Jonathan Jacob Carver, Rachael Riley Denbrock, Cindy Carolina Martines, Alessandro Didonna   +3 more
wiley   +1 more source

Ion Mobility Gas-Phase Separation Enhances Top-Down Mass Spectrometry of Heavily Modified Guide RNA. [PDF]

Anal Chem
Macias LA, Lowther J, Tillotson EL, Rohde E, Madsen JA.   +4 more
europepmc   +1 more source

Calcineurin controls the expression of numerous genes in cerebellar granule cells [PDF]

, 2003
Ashby, Dominique S, Freeman, Tom C, Fresu, Luigia, Genazzani, Armando A, Kramer, Dana   +4 more
core   +1 more source

A guide to building the matrisome interactome: from computational predictions to experimental validation

The FEBS Journal, EarlyView.
This guide proposes a roadmap combining state‐of‐the‐art computational and experimental approaches (including biophysical, biochemical, and imaging techniques) to identify, characterize, and contextualize extracellular matrix (ECM) protein interactions.
Leanna Leverton, Amanpreet Kaur Bains, Ikram Isa, Sylvie Ricard‐Blum, Alexandra Naba   +4 more
wiley   +1 more source

Plant organelle C‐to‐U RNA editing factors can operate successfully in yeast (Saccharomyces cerevisiae) as an easily amenable eukaryotic system for their functional analysis

The FEBS Journal, EarlyView.
Plant‐type pentatricopeptide repeat proteins capable of C‐to‐U RNA editing perform faithfully when expressed in a new heterologous system, the yeast Saccharomyces cerevisiae. They were tested with constitutive and inducible expression and with a set of different solubility tags. PPR56, PPR65, and PPR78 from P.
Shyam Ramanathan, Annika Hanraths, Jingchan Xie, Mareike Schallenberg‐Rüdinger, Volker Knoop   +4 more
wiley   +1 more source

Subtelomeric elements provide stability to short telomeres in telomerase-negative cells of the budding yeast Naumovozyma castellii. [PDF]

Curr Genet
Jaiswal RK, Garibo Domingo T, Grunchec H, Singh K, Pirooznia M, Elhaik E, Cohn M.   +6 more
europepmc   +1 more source

EHD4 and ASAP2 are critical negative regulators of the claudin‐5‐based endothelial barrier

The FEBS Journal, EarlyView.
Cell‐surface CLDN‐5 protein levels can be evaluated using a probe that can bind to the extracellular domains of CLDN‐5. A probe derived from Clostridium perfringens enterotoxin allows us to isolate cells with high CLDN‐5 protein levels from a knockout cell library.
Yosuke Hashimoto, Gergő Porkoláb, Natalie Hudson, Jeffrey O'Callaghan, Nicole Hanley, Conor Delaney, Mária A. Deli, Peter Westenskow, Matthew Campbell   +8 more
wiley   +1 more source

A Rapid, High-Throughput Method for the Construction of Mutagenesis Libraries. [PDF]

Biomolecules
Lu Y, Meng S, Guan X, He P, Zhao D.
europepmc   +1 more source

Molecular Testing in Sickle Cell Disease: From Newborn Screening to Transfusion Care

International Journal of Laboratory Hematology, EarlyView.
ABSTRACT Sickle cell disease (SCD) is one of the most frequent monogenic diseases worldwide and a highly heterogeneous and complex disease. SCD care carries several challenges. This includes early and accurate diagnosis as well as optimal red blood cell transfusion matching in this population carrying a high risk of alloimmunization.
Thomas Pincez, Yves D. Pastore
wiley   +1 more source

Comprehensive web-based platform for advanced PCR design, genotyping, synthetic biology, molecular diagnostics, and sequence analysis. [PDF]

Mol Ther Nucleic Acids
Kalendar R.
europepmc   +1 more source