Results 41 to 50 of about 708,274 (274)
Virome Capture Sequencing Enables Sensitive Viral Diagnosis and Comprehensive Virome Analysis
mBio, 2015 Insensitivity and technical complexity have impeded the implementation of high-throughput nucleic acid sequencing in differential diagnosis of viral infections in clinical laboratories.
T. Briese +11 more
semanticscholar +1 more source
Solving the riddle of the bright mismatches: hybridization in oligonucleotide arrays [PDF]
, 2002 HDONA technology is predicated on two ideas. First, the differential between high-affinity (perfect match, PM) and lower-affinity (mismatch, MM) probes is used to minimize cross-hybridization. Second, several short probes along the transcript are combined, introducing redundancy.
arxiv +1 more source
Single-molecule DNA Bases Discrimination in Oligonucleotides by Controllable Trapping in Plasmonic Nanoholes [PDF]
Nature Communications 10, 5321 (2019), 2019 Surface-enhanced Raman spectroscopy (SERS) sensing of DNA sequences by plasmonic nanopores could pave a way to new generation single-molecule sequencing platforms. The SERS discrimination of single DNA bases depends critically on the time that a DNA strand resides within the plasmonic hot spot.
arxiv +1 more source
High-Throughput Methods for Detection of Genetic Variation
BioTechniques, 2001 Understanding human genetic variation is currently believed to reveal the cause of individual susceptibility to disease and the large variation observed in response to treatment.
Vessela Nedelcheva Kristensen +3 more
doaj +1 more source
Molecular Oncology, EarlyView.Tumor microenvironment drives cancer formation and progression. We analyzed the role of human cancer‐associated adipocytes from patients with renal cell carcinoma (RCC) stratified as lean, overweight, or obese. RNA‐seq demonstrated that, among the most altered genes involved in the tumor–stroma crosstalk, are ADAM12 and CYP1B1, which were proven to be ...
Sepehr Torabinejad +13 more
wiley +1 more source
DNA detection by THz pumping [PDF]
, 2014 Our results demonstrate a new method for label-free, real-time oligonucleotide characterisation by their self-resonant modes, which are unique to their conformation and sequence. We anticipate that our assay will be used as a starting point for a more detailed investigation of the aforementioned mechanism, which can be used as a basis for ...
arxiv +1 more source
Au-Ag template stripped pattern for scanning probe investigations of DNA arrays produced by Dip Pen Nanolithography [PDF]
Langmuir 24, 13212-13217 (2008), 2008 We report on DNA arrays produced by Dip Pen Nanolithography (DPN) on a novel Au-Ag micro patterned template stripped surface. DNA arrays have been investigated by atomic force microscopy (AFM) and scanning tunnelling microscopy (STM) showing that the patterned template stripped substrate enables easy retrieval of the DPN-functionalized zone with a ...
arxiv +1 more source
High-throughput Microarray Detection of Vomeronasal Receptor Gene Expression in Rodents
Frontiers in Neuroscience, 2010 We performed comprehensive data mining to explore the vomeronasal receptor (V1R & V2R) repertoires in mouse and rat using the mm5 and rn3 genome, respectively.
Xiaohong Zhang +2 more
doaj +1 more source
Genome Research, 2003 We have developed a methodology we call ROMA (representational oligonucleotide microarray analysis), for the detection of the genomic aberrations in cancer and normal humans. By arraying oligonucleotide probes designed from the human genome sequence, and
R. Lucito +18 more
semanticscholar +1 more source
Inhibitor of DNA binding‐1 is a key regulator of cancer cell vasculogenic mimicry
Molecular Oncology, EarlyView.Elevated expression of transcriptional regulator inhibitor of DNA binding 1 (ID1) promoted cancer cell‐mediated vasculogenic mimicry (VM) through regulation of pro‐angiogenic and pro‐cancerous genes (e.g. VE‐cadherin (CDH5), TIE2, MMP9, DKK1). Higher ID1 expression also increased metastases to the lung and the liver.
Emma J. Thompson +11 more
wiley +1 more source