Results 71 to 80 of about 149,559 (187)

Custom Design and Analysis of High-Density Oligonucleotide Bacterial Tiling Microarrays

PLoS ONE, 2009
Background High-density tiling microarrays are a powerful tool for the characterization of complete genomes. The two major computational challenges associated with custom-made arrays are design and analysis.
G. Thomassen, A. Rowe, Karin Lagesen, Jessica M. Lindvall, Torbjørn Rognes   +4 more
semanticscholar   +1 more source

Site Selective Antibody-Oligonucleotide Conjugation via Microbial Transglutaminase. [PDF]

, 2019
Nucleic Acid Therapeutics (NATs), including siRNAs and AntiSense Oligonucleotides (ASOs), have great potential to drug the undruggable genome. Targeting siRNAs and ASOs to specific cell types of interest has driven dramatic improvement in efficacy and ...
Cui, Xianshu, Dowdy, Steven F, Huggins, Ian J, Jadhav, Satish, Medina, Carlos A, Springer, Aaron D, van den Berg, Arjen   +6 more
core   +1 more source

Improved detection of global copy number variation using high density, non-polymorphic oligonucleotide probes

BMC Genetics, 2008
Background DNA sequence diversity within the human genome may be more greatly affected by copy number variations (CNVs) than single nucleotide polymorphisms (SNPs).
Liu Guoying, Zhang Jane, Chen Wenwei, Kirby Andrew, Truong Vivi B, Fitch Karen R, Huang Jing, Shen Fan, McCarroll Steven A, Jones Keith W, Shapero Michael H   +10 more
doaj   +1 more source

Combinatorial CRISPR-Cas9 screens for de novo mapping of genetic interactions. [PDF]

, 2017
We developed a systematic approach to map human genetic networks by combinatorial CRISPR-Cas9 perturbations coupled to robust analysis of growth kinetics.
A Baryshnikova, A Baryshnikova, A DeLean, Aaron N Chang, AE Briner, Alex N Beckett, Alex Thomas, AM Kabadi, Amanda Birmingham, Ana Bojorquez-Gomez, ASL Wong, Assen Roguev, B Vogelstein, C Laufer, Chih-Chung Kuo, CJ Lord, Dan Du, Daniel Pekin, Dongxin Zhao, F Lori, H Xu, J Shi, Jason F Kreisberg, JD Storey, Jens Luebeck, JG Doench, John Paul Shen, Katherine Licon, Kristin Klepper, KS Pollard, Kyle Salinas Sanchez, L Avery, LA Gilbert, Lei Qi, M Costanzo, M Martin, MC Bassik, MS Neshat, Nathan E Lewis, NE Sanjana, Nevan Krogan, NJ Krogan, P Mali, P Mali, Prashant Mali, R Chari, R Mani, R Srivas, Roman Sasik, S Bandyopadhyay, SR Collins, T Hart, T Horn, Trey Ideker, VG Tusher, Y Benjamini, Y Dang   +56 more
core   +1 more source

In vivo transcriptional profiling of Plasmodium falciparum

Malaria Journal, 2004
Background Both host and pathogen factors contribute to disease outcome in Plasmodium falciparum infection. The feasibility of studying the P. falciparum in vivo transcriptome to understand parasite transcriptional response while it resides in the human ...
Sultan Ali, Mboup Soulyemane, Ndir Omar, Zhou Yingyao, Fang Xuemin, Sarr Ousmane, Le Roch Karine G, Daily Johanna P, Winzeler Elizabeth A, Wirth Dyann F   +9 more
doaj   +1 more source

DNA methylation profiling of primary neuroblastoma tumors using methyl-CpG-binding domain sequencing [PDF]

, 2016
Comprehensive genome-wide DNA methylation studies in neuroblastoma (NB), a childhood tumor that originates from precursor cells of the sympathetic nervous system, are scarce. Recently, we profiled the DNA methylome of 102 well-annotated primary NB tumors
Decock, Anneleen, Ongenaert, Maté, Speleman, Franki, Van Criekinge, Wim, Vandesompele, Jo   +4 more
core   +1 more source

Transcriptional analysis of pqqD and study of the regulation of pyrroloquinoline quinone biosynthesis in Methylobacterium extorquens AM1 [PDF]

, 1995
Methanol dehydrogenase, the enzyme that oxidizes methanol to formaldehyde in gram-negative methylotrophs, contains the prosthetic group pyrroloquinoline quinone (PQQ).
Lidstrom, Mary E., Ramamoorthi, Roopa
core   +2 more sources

Microarray MAPH: accurate array-based detection of relative copy number in genomic DNA

BMC Genomics, 2006
Background Current methods for measurement of copy number do not combine all the desirable qualities of convenience, throughput, economy, accuracy and resolution.
Chan Alan, Wu Ying, Datta Parikkhit, Gibbons Brian, AL Armour John   +4 more
doaj   +1 more source

A Newly Discovered Bordetella Species Carries a Transcriptionally Active CRISPR-Cas with a Small Cas9 Endonuclease [PDF]

, 2015
Background Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated genes (cas) are widely distributed among bacteria.
Dudley, Edward G., Harvill, Eric T., Hu, Kai, Ivanov, Yury V., Linz, Bodo, Register, Karen B., Rivera, Israel, Shariat, Nikki   +7 more
core   +2 more sources

Oligonucleotide array discovery of polymorphisms in cultivated tomato (Solanum lycopersicum L.) reveals patterns of SNP variation associated with breeding

BMC Genomics, 2009
Background Cultivated tomato (Solanum lycopersicum L.) has narrow genetic diversity that makes it difficult to identify polymorphisms between elite germplasm.
Zhu Tong, Chilcott Charles, Robbins Matthew D, Sim Sung-Chur, Francis David M   +4 more
doaj   +1 more source