Results 91 to 100 of about 80,761 (229)

CREAT: A CRISPR‐Based Genome Trimming Strategy for Systematic Identification of Dispensable Regions and Rapid Genome Reduction

Advanced Science, EarlyView.
Current genome‐reduction methods work in a trial‐and‐error fashion, constituting a long‐standing bottleneck in the research. We developed CREAT (CRISPR‐based genome trimming with a multi‐homology‐arm template), a CRISPR‐based genetic approach that enables systematically classifying essential versus non‐essential genomic regions and subsequently ...
Guanhua Yuan, Zhe Gao, Yingxuan Qi, Yang Zhang, Xuhui Tian, Pengpeng Zhao, Xu Feng, Qunxin She   +7 more
wiley   +1 more source

Discovery of novel variants in genotyping arrays improves genotype retention and reduces ascertainment bias

BMC Genomics, 2012
Background High-density genotyping arrays that measure hybridization of genomic DNA fragments to allele-specific oligonucleotide probes are widely used to genotype single nucleotide polymorphisms (SNPs) in genetic studies, including human genome-wide ...
Didion John P, Yang Hyuna, Sheppard Keith, Fu Chen-Ping, McMillan Leonard, de Villena Fernando, Churchill Gary A   +6 more
doaj   +1 more source

Membrane Fusion‐Mediated Cytosolic Delivery of Threose Nucleic Acids via Homotypic Nanoparticles Overcomes Drug Resistance in Triple‐Negative Breast Cancer

Advanced Science, EarlyView.
This study introduces a biomimetic “nanofusion” platform that integrates the biostability of threose nucleic acids (TNA) with homotypic cell‐membrane cloaking to combat drug‐resistant TNBC. By leveraging a non‐canonical membrane‐fusion pathway for direct cytosolic delivery, the platform bypasses endosomal sequestration. To achieve potent AKT2 silencing
Wei Zheng, Tristan Juin Han Chang, Xinchao Li, Zhongqi Zhou, Chung Tin, Kenward Vong, Pierre Karam, Pik Kwan Lo   +7 more
wiley   +1 more source

Meta-analysis of breast cancer microarray studies in conjunction with conserved cis-elements suggest patterns for coordinate regulation

BMC Bioinformatics, 2008
Background Gene expression measurements from breast cancer (BrCa) tumors are established clinical predictive tools to identify tumor subtypes, identify patients showing poor/good prognosis, and identify patients likely to have disease recurrence. However,
Lundberg Cathryn, Snøve Ola, Sætrom Pål, Smith David D, Rivas Guillermo E, Glackin Carlotta, Larson Garrett P   +6 more
doaj   +1 more source

Application of multiple response optimization design to quantum dot-encoded microsphere bioconjugates hybridization assay [PDF]

, 2011
The optimization of DNA hybridization for genotyping assays is a complex experimental problem that depends on multiple factors such as assay formats, fluorescent probes, target sequence, experimental conditions, and data analysis.
Morgan, Sarah L., Sarah Thiollet, Bessant, Conrad M., Thiollet, Sarah, Conrad Bessant, Sarah L. Morgan   +5 more
core   +1 more source

TDP‐43 Aggregation: The Healthy‐Toxic Balance of the Prion‐Like Domain

Advanced Science, EarlyView.
TDP‐43 function relies on a delicate balance between reversible phase‐separated states and irreversible aggregation. Under physiological conditions, TDP‐43 forms dynamic droplets and oligomers that support normal cellular functions. In pathological contexts, this balance shifts toward aberrant aggregation, leading to toxic species.
Luca Zangrando, Emanuele Buratti, Francesca Paron   +2 more
wiley   +1 more source

Mitochondrial DNA mutations in preneoplastic lesions of the gastrointestinal tract: A biomarker for the early detection of cancer

Molecular Cancer, 2006
Background Somatic mutations of mitochondrial DNA (mtDNA) are common in many human cancers. We have described an oligonucleotide microarray ("MitoChip") for rapid sequencing of the entire mitochondrial genome (Zhou et al, J Mol Diagn 2006), facilitating ...
Montgomery Elizabeth A, Eshleman James R, Lee Edward E, Canto Marcia, Wang Jean, Zhou Shaoyu, Sui Guoping, Sidransky David, Califano Joseph A, Maitra Anirban   +9 more
doaj   +1 more source

Optimised padlock probe ligation and microarray detection of multiple (non-authorised) GMOs in a single reaction [PDF]

, 2008
Background To maintain EU GMO regulations, producers of new GM crop varieties need to supply an event-specific method for the new variety. As a result methods are nowadays available for EU-authorised genetically modified organisms (GMOs), but only to a ...
Aarts, H.J.M., AM Angeline Van Hoef, van Dijk, J.P., van Hoef, A.M.A., Kok, E.J., Esther J Kok, Henriek G Beenen, Schoen, C.D., Beenen, H.G., Cor D Schoen, Henk JM Aarts, Marleen M Voorhuijzen, Hoef, A.M.A., van, Jeroen P van Dijk, Theo W Prins, Dijk, J.P., van, Prins, T.W., Voorhuijzen, M.M.   +17 more
core   +1 more source

Inhibition of KDEL Receptors Remodels the Tumor Microenvironment for T Cell Independent Tumor Regression

Advanced Science, EarlyView.
Inhibition of KDELR2 in a small fraction of tumor cells generates sustainable immunogenic cell death conditions within the tumor microenvironment. These conditions promote the regression of tumors in a T cell independent manner. During regression, macrophages prime T cells that subsequently provide systemic protection against recurrence. The potency of
Shakti P Pattanayak, Hong Wang, Belinda Willard, Timothy A. Chan, William C Merrick, Zheng‐Rong Lu, Boaz Tirosh   +6 more
wiley   +1 more source

MIMAS 3.0 is a Multiomics Information Management and Annotation System

BMC Bioinformatics, 2009
Background DNA sequence integrity, mRNA concentrations and protein-DNA interactions have been subject to genome-wide analyses based on microarrays with ever increasing efficiency and reliability over the past fifteen years.
Rougemont Jacques, Collin Olivier, Xenarios Ioannis, Liechti Robin, Hermida Leandro, Gattiker Alexandre, Primig Michael   +6 more
doaj   +1 more source