Results 81 to 90 of about 80,761 (229)

Oligonucleotide array discovery of polymorphisms in cultivated tomato (Solanum lycopersicum L.) reveals patterns of SNP variation associated with breeding

BMC Genomics, 2009
Background Cultivated tomato (Solanum lycopersicum L.) has narrow genetic diversity that makes it difficult to identify polymorphisms between elite germplasm.
Zhu Tong, Chilcott Charles, Robbins Matthew D, Sim Sung-Chur, Francis David M   +4 more
doaj   +1 more source

An Integrated DNA Nanoprobe for Intranuclear Imaging and in Situ Profiling of OGG1 Activity

Advanced Science, EarlyView.
The TP‐SA nanoprobe, integrating an AS1411 aptamer for nuclear delivery and a FRET array for signal amplification, enables real‐time tracking of nuclear OGG1 activity. It reveals cell‐line‐specific basal OGG1 levels and shows clinical promise using pneumonia patients’ lavage fluid.
Mingzhu Zhao, Xuemei Sun, He Li, Bangming Wang, Mengting Pan, Zhen Ma, Rong‐Mei Kong, Weiheng Kong, Yan Zhao, Fengli Qu   +9 more
wiley   +1 more source

Discovery and mapping of single feature polymorphisms in wheat using Affymetrix arrays

BMC Genomics, 2009
Background Wheat (Triticum aestivum L.) is a staple food crop worldwide. The wheat genome has not yet been sequenced due to its huge genome size (~17,000 Mb) and high levels of repetitive sequences; the whole genome sequence may not be expected in the ...
Hu Shengwa, Ma Hongxiang, Bradbury Peter J, Bernardo Amy N, Bowden Robert L, Buckler Edward S, Bai Guihua   +6 more
doaj   +1 more source

A pilot study of transcription unit analysis in rice using oligonucleotide tiling-path microarray [PDF]

, 2005
As the international efforts to sequence the rice genome are completed, an immediate challenge and opportunity is to comprehensively and accurately define all transcription units in the rice genome.
Li, L, Li, Jiayang, Li, Xueyong, Xue, YB, Gerstein, M, Xue, Yongbiao, Wang, J, Tongprasit, W, Stolc, Viktor, Tongprasit, Waraporn, Deng, XW, Li, XY, Su, N, Han, B, Han, Bin, Stolc, V, Deng, Xing Wang, Wang, Xiangfeng, Snyder, Michael, Li, JY, Wang, Jun, Snyder, M, Gerstein, Mark, Wang, XF, Li, Lei, Su, Ning   +25 more
core   +1 more source

Discovery and Engineering of a Rat Endogenous Retrovirus Reverse Transcriptase for Efficient Prime Editing

Advanced Science, EarlyView.
We screened 558 reverse transcriptases and engineered an optimized rat endogenous retrovirus‐derived variant, enRERV‐RT, via structure‐guided engineering and deep mutational scanning. This enhanced prime editor, based on the engineered RT, outperforms conventional M‐MLV‐RT systems across plant and animal cells, particularly at hard‐to‐edit loci ...
Linsha Ma, Pengcheng Yao, Shengqi Wu, Yuanyuan Shi, Lang Qin, Baitao Li, Jiayi Zhu, Minhua Huang, Yichong Zhu, Yuwen Song, Jinhuan Pang, Ziping Guo, Guochuan Wu, Chen Wang, Kewei Xu, Ruihua Huang, Quan Kuang, Liang Qu, Changtian Pan, Xianrong Xie, Qinlong Zhu, Jiaying Huang, Qiupeng Lin   +22 more
wiley   +1 more source

Microarray-based analysis of differential gene expression between infective and noninfective larvae of Strongyloides stercoralis.

PLoS Neglected Tropical Diseases, 2011
BackgroundDifferences between noninfective first-stage (L1) and infective third-stage (L3i) larvae of parasitic nematode Strongyloides stercoralis at the molecular level are relatively uncharacterized. DNA microarrays were developed and utilized for this
Roshan Ramanathan, Sudhir Varma, José M C Ribeiro, Timothy G Myers, Thomas J Nolan, David Abraham, James B Lok, Thomas B Nutman   +7 more
doaj   +1 more source

Comparative analysis of pyrosequencing and a phylogenetic microarray for exploring microbial community structures in the human distal intestine [PDF]

, 2009
BACKGROUND: Variations in the composition of the human intestinal microbiota are linked to diverse health conditions. High-throughput molecular technologies have recently elucidated microbial community structure at much higher resolution than was ...
Vos, W.M., de, Willem M de Vos, O\u27Toole, Paul W., Smidt, H., O'Sullivan, O., Janne Nikkilä, Claesson Marcus J., de Vos Willem M., Hauke Smidt, O'Sullivan, Orla, Marchesi, Julian Roberto, O\u27Sullivan, Orla, Ross R. Paul, Smidt, Hauka, Ross, R. Paul, Ross, R. P., Paul W O'Toole, Wang, Qiong, Orla O'Sullivan, O'Sullivan Orla, de Vos, W.M., Qiong Wang, Nikkilä Janne, O'Toole, Paul W., Claesson, M.J., Marchesi, J.R., O'Toole, P.W., Claesson, Marcus J., O'Toole, P. W., Wang, Q., de Vos, Willem M., Claesson, M. J., Julian R Marchesi, O'Toole Paul W., Marchesi, Julian R., Smidt, Hauke, Ross, R.P., Smidt Hauke, Nikkila, Janne, R Paul Ross, Marchesi Julian R., Ross, R Paul, Wang Qiong, De Vos, W. M., Nikkilä, J., Marcus J Claesson   +45 more
core   +1 more source

Machine Learning‐Enhanced Ultrasensitive Immuno‐CRISPR Array Facilitates Early Diagnosis of Alzheimer's Disease by Detecting Multiple Plasma Biomarkers

Advanced Science, EarlyView.
This work presents a CRISPR‐based, ultrasensitive multiplex protein detection array capable of simultaneously analyzing six plasma biomarkers associated with Alzheimer's disease (AD). By integrating antibody‐mediated signal transduction, recombinase polymerase amplification, and spatially encoded CRISPR‐Cas12a, the system achieves detection sensitivity
Liding Zhang, Changwen Yang, Qian Yao, Xuewei Du, Shuai Ding, Yaoqiang Shi, Can Sheng, Ming Wang, Ying Han, Haiming Luo   +9 more
wiley   +1 more source

Gene expression analysis identifies hypothetical genes that may be critical during the infection process of Xanthomonas citri subsp. citri

Electronic Journal of Biotechnology, 2019
Background: Gene expression analysis via microarray is widely used in phytobacteria to validate differential gene expression associated with virulence or to compare biological profiles of wild type and mutant strains. Here, we employed DNA microarrays to
Marcelo Luiz de Laia, Leandro Marcio Moreira, Janaína Fernandes Gonçalves, Maria Inês Tiraboschi Ferro, Any Caroliny Pinto Rodrigues, Jéssica Naiara dos Santos, Érica Barbosa Felestrino, Jesus Aparecido Ferro   +7 more
doaj   +1 more source

Combined Oligonucleotide and Protein Microarray Temporal Analysis of p53-mediated Apoptosis [PDF]

, 2005
Background: P53-mediated apoptosis involves a complex process induced largely by p53 acting as a transcription factor. We hypothesised that p53 expression would lead to transcriptional events that rapidly change during apoptosis and that protein array ...
Bailey A., Wee E., Lambe T., Ogg G.S., Jones L., Black A.P.   +5 more
core