Results 81 to 90 of about 708,274 (274)

A study of accuracy and precision in oligonucleotide arrays: extracting more signal at large concentrations [PDF]

arXiv, 2002
Despite the success and popularity of oligonucleotide arrays as a high-throughput technique for measuring mRNA expression levels, quantitative calibration studies have until now been limited. The main reason is that suitable data was not available. However, calibration data recently produced by Affymetrix now permits detailed studies of the intensity ...
arxiv  

Prokaryotic RNA preparation methods useful for high density array analysis: comparison of two approaches.

Nucleic Acids Research, 2001
High density oligonucleotide arrays have been used extensively for expression studies of eukaryotic organisms. We have designed a prokaryotic high density oligonucleotide array using the complete Escherichia coli genome sequence to monitor expression ...
C. Rosenow, R. Saxena, M. Durst, T. Gingeras   +3 more
semanticscholar   +1 more source

Dynamics of Cell Fate Decisions during Chemically Induced Multi‐Lineage Trans‐Differentiation at Single‐Cell Level

Advanced Science, EarlyView.
A critical aspect of chromosomal remodeling in small molecule‐induced multilineage trans‐differentiation(iMT) is elucidated. A dynamic atlas spanning over 35 days profiles terminal trans‐differentiated cells, which reverted to a more youthful state. This study reveals parallels between iMT and other cell fate determination processes, emphasizing an ...
Weigao E, Lijiang Fei, Jingjing Wang, Xinru Wang, Renying Wang, Xueyi Wang, Peijing Zhang, Jianhui Chen, Junqing Wu, Mengmeng Jiang, Daosheng Huang, Danmei Jia, Guoji Guo, Xiaoping Han   +13 more
wiley   +1 more source

Identification of candidate regulatory sequences in mammalian 3' UTRs by statistical analysis of oligonucleotide distributions [PDF]

BMC Bioinformatics. 2007 May 24;8:174. PMID: 17524134, 2007
3' untranslated regions (3' UTRs) contain binding sites for many regulatory elements, and in particular for microRNAs (miRNAs). The importance of miRNA-mediated post-transcriptional regulation has become increasingly clear in the last few years. We propose two complementary approaches to the statistical analysis of oligonucleotide frequencies in ...
arxiv  

Microarray MAPH: accurate array-based detection of relative copy number in genomic DNA

BMC Genomics, 2006
Background Current methods for measurement of copy number do not combine all the desirable qualities of convenience, throughput, economy, accuracy and resolution.
Chan Alan, Wu Ying, Datta Parikkhit, Gibbons Brian, AL Armour John   +4 more
doaj   +1 more source

Accurate DNA fragment sizing by capillary electrophoresis with laser-induced fluorescence array for detection of sequence specificity of DNA damage.

Analytical Chemistry, 2008
Cancer has been linked to mutations within specific codons in genes that code for critical biomolecules such as tumor suppressor proteins (e.g., p53). Activated metabolites like benzo[a]pyrenediol epoxide act on preferred nucleotide sequences of DNA, and
Erwin V Fundador, D. Choudhary, J. Schenkman, J. Rusling   +3 more
semanticscholar   +1 more source

DNA Origami‐Cyanine Nanocomplex for Precision Imaging of KRAS‐Mutant Pancreatic Cancer Cells

Advanced Science, EarlyView.
DNA origami‐cyanine (Do‐Cy) nanocomplexes are synthesized to selectively image KRAS‐mutant pancreatic cancer cells in the highly desmoplastic tumors. This study shows that the Do‐Cy nanocomplexes are preferentially internalized by cancer cells over other stroma cells via KRAS‐dependent macropinocytosis.
Hye‐ran Moon, Yancheng Du, Sae Rome Choi, Seongmin Seo, Cih Cheng, Bennett D. Elzey, Jong Hyun Choi, Bumsoo Han   +7 more
wiley   +1 more source

Mosaicism in tuberous sclerosis complex: Lowering the threshold for clinical reporting

Human Mutation, Volume 43, Issue 12, Page 1956-1969, December 2022., 2022
Abstract Tuberous sclerosis complex (TSC) is a multi‐system genetic disorder. Most patients have germline mutations in TSC1 or TSC2 but, 10%–15% patients do not have TSC1/TSC2 mutations detected on routine clinical genetic testing. We investigated the contribution of low‐level mosaic TSC1/TSC2 mutations in unsolved sporadic patients and families with ...
Zimeng Ye, Sufang Lin, Xia Zhao, Mark F. Bennett, Natasha J. Brown, Mathew Wallis, Xinyi Gao, Li Sun, Jiarui Wu, Ravikiran Vedururu, Tom Witkowski, Fiona Gardiner, Chloe Stutterd, Jing Duan, Saul A. Mullen, George McGillivray, Simon Bodek, Giulia Valente, Matthew Reagan, Yi Yao, Lin Li, Li Chen, Amber Boys, Thiuni N. Adikari, Dezhi Cao, Zhanqi Hu, Victoria Beshay, Victor W. Zhang, Samuel F. Berkovic, Ingrid E. Scheffer, Jianxiang Liao, Michael S. Hildebrand   +31 more
wiley   +1 more source

Oligonucleotide array discovery of polymorphisms in cultivated tomato (Solanum lycopersicum L.) reveals patterns of SNP variation associated with breeding

BMC Genomics, 2009
Background Cultivated tomato (Solanum lycopersicum L.) has narrow genetic diversity that makes it difficult to identify polymorphisms between elite germplasm.
Zhu Tong, Chilcott Charles, Robbins Matthew D, Sim Sung-Chur, Francis David M   +4 more
doaj   +1 more source

Analysis of clinically relevant single-nucleotide polymorphisms by use of microelectronic array technology.

Clinical Chemistry, 2002
BACKGROUND Microelectronic DNA chip devices represent an emerging technology for genotyping. We developed methods for detection of single-nucleotide polymorphisms (SNPs) in clinically relevant genes.
R. Santacroce, A. Ratti, F. Caroli, B. Foglieni, A. Ferraris, L. Cremonesi, M. Margaglione, M. Seri, R. Ravazzolo, G. Restagno, B. Dallapiccola, E. Rappaport, E. Pollak, S. Surrey, M. Ferrari, P. Fortina   +15 more
semanticscholar   +1 more source