Results 41 to 50 of about 1,424,575 (357)

Imaging individual mRNA molecules using multiple singly labeled probes

Nature Methods, 2008
We describe a method for imaging individual mRNA molecules in fixed cells by probing each mRNA species with 48 or more short, singly labeled oligonucleotide probes.
A. Raj, P. V. D. van den Bogaard, Scott A. Rifkin, A. van Oudenaarden, Sanjay Tyagi   +4 more
semanticscholar   +1 more source

Oligonucleotides as probes for mammalian protamine mRNAs

Bioscience Reports, 1986
Protamine-like sequences have been identified in poly A(+) mRNAs from mammalian testes by the use of a common, complementary oligonucleotide (GCAGCANCK PTANCKNGCCAT; predicted from the common N-terminal amino acid sequence, MARYRCC, seen in several mammalian P1 protamines [D. J. McKay, B. S. Renaux and G. H. Dixon, Bioscience Reports5:383–391 (1985)]).
Gordon H. Dixon, Wayne Connor, Stephen A. Krawetz   +2 more
openaire   +3 more sources

ShcD adaptor protein drives invasion of triple negative breast cancer cells by aberrant activation of EGFR signaling

Molecular Oncology, EarlyView.
We identified adaptor protein ShcD as upregulated in triple‐negative breast cancer and found its expression to be correlated with reduced patient survival and increased invasion in cell models. Using a proteomic screen, we identified novel ShcD binding partners involved in EGFR signaling pathways.
Hayley R. Lau, Hayley S. Smith, Begüm Alural, Claire E. Martin, Laura A. New, Manali Tilak, Sara L. Banerjee, Hannah N. Robeson, Nicolas Bisson, Anne‐Claude Gingras, Jasmin Lalonde, Nina Jones   +11 more
wiley   +1 more source

Effect of Secondary Structure on Single Nucleotide Polymorphism Detection with a Porous Microarray Matrix; Implications for Probe Selection

BioTechniques, 2003
Oligonucleotide arrays capable of detecting single nucleotide polymorphisms (SNPs) from amplified nucleic acid have many applications. The expected SNP is usually placed approximately in the center of the probe to ensure the maximum shift in Tm between ...
R.M. Anthony, A.R.J. Schuitema, A.B. Chan, P.J. Boender, P.R. Klatser, L. Oskam   +5 more
doaj   +1 more source

GCSscore: an R package for differential gene expression analysis in Affymetrix/Thermo-Fisher whole transcriptome microarrays

BMC Genomics, 2021
Background Despite the increasing use of RNAseq for transcriptome analysis, microarrays remain a widely-used methodology for genomic studies. The latest generation of Affymetrix/Thermo-Fisher microarrays, the ClariomD/XTA and ClariomS array, provide a ...
Guy M. Harris, Shahroze Abbas, Michael F. Miles   +2 more
doaj   +1 more source

Inhibitor of DNA binding‐1 is a key regulator of cancer cell vasculogenic mimicry

Molecular Oncology, EarlyView.
Elevated expression of transcriptional regulator inhibitor of DNA binding 1 (ID1) promoted cancer cell‐mediated vasculogenic mimicry (VM) through regulation of pro‐angiogenic and pro‐cancerous genes (e.g. VE‐cadherin (CDH5), TIE2, MMP9, DKK1). Higher ID1 expression also increased metastases to the lung and the liver.
Emma J. Thompson, Emma L. Dorward, Kristyn Jurrius, Nathalie Nataren, Markus Tondl, Kay K. Myo Min, Michaelia P. Cockshell, Anahita Fouladzadeh, John Toubia, Mark DeNichilo, Delphine Merino, Claudine S. Bonder   +11 more
wiley   +1 more source

Effect of Cholesterol Anchoring Group on the Properties of G-Quadruplex-Based FRET Probes for Potassium Ion

Chemosensors, 2014
In this study we report on the spectral properties and G-quadruplex folding ability of fluorescent oligonucleotide probes modified by the attachment of a cholesterol moiety.
Angelika Swiatkowska, Bernard Juskowiak
doaj   +1 more source

Oligonucleotide primers, probes and molecular methods for the environmental monitoring of methanogenic archaea

Microbial Biotechnology, 2011
For the identification and quantification of methanogenic archaea (methanogens) in environmental samples, various oligonucleotide probes/primers targeting phylogenetic markers of methanogens, such as 16S rRNA, 16S rRNA gene and the gene for the α‐subunit
T. Narihiro, Y. Sekiguchi
semanticscholar   +1 more source

The anticancer effect of the HDAC inhibitor belinostat is enhanced by inhibitors of Bcl‐xL or Mcl‐1 in ovarian cancer

Molecular Oncology, EarlyView.
The pan‐HDAC inhibitor belinostat increases the expression of the pro‐apoptotic proteins Bim, Puma, and Noxa and induces apoptosis in ovarian cancer cell lines and patient‐derived tumor organoids when used at high concentrations. Moreover, inhibiting the anti‐apoptotic proteins Bcl‐xL or Mcl‐1 sensitizes these preclinical models to the cytotoxic effect
Cécilia Thomine, Sterenn Guillemot, Louis‐Bastien Weiswald, Romane Florent, Edwige Abeilard, Florence Giffard, Emilie Brotin, Mélanie Briand, Enora Dolivet, Laurent Poulain, Marie Villedieu   +10 more
wiley   +1 more source

Development and evaluation of new mask protocols for gene expression profiling in humans and chimpanzees

BMC Bioinformatics, 2009
Background Cross-species gene expression analyses using oligonucleotide microarrays designed to evaluate a single species can provide spurious results due to mismatches between the interrogated transcriptome and arrayed probes.
Siegmund Kimberly D, Wildman Derek E, Islam Munirul, Wolfsberg Tyra G, Renaud Gabriel, Toleno Donna M, Hacia Joseph G   +6 more
doaj   +1 more source